Restriction map for
pF1KE2392
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AviII AvrII BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DraI DraIII DrdI EcoRI FbaI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 660 GGCGCC G[GA]^CG[CT]C
AhdI 1105 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1057 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1233 AGCGCT AGC^GCT
ApaI 56 GGGCCC GGGCC^C
AspEI 1105 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 267 GGATCC G^GATCC
BanII 56 GGGCCC G[GA]GC[CT]^C
BbeI 660 GGCGCC GGCGC^C
BcgI 383 GCAATGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1246 CTAG C^TAG
BglI 1309 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1269 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1269 GCTGAGC GC^T[ACGT]AGC
BsaHI 660 GGCGCC G[GA]^CG[CT]C
BsaI 995 GGTCTC GGTCTC(1/5)
BsgI 1099 GTGCAG GTGCAG(16/14)
BsiEI 325 CGGCCG CG[GA][CT]^CG
BsmI 963 GAATGC GAATGC(1/-1)
BsrFI 1500 GCCGGC [GA]^CCGG[CT]
BssSI 391 CTCGTG CACGAG(-5/-1)
BstEII 942 GGTGACC G^GT[ACGT]ACC
BstPI 942 GGTGACC G^GT[ACGT]ACC
BstXI 1275 CCAGTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1269 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1500 GCCGGC [GA]^CCGG[CT]
DraII 55 GGGGCCC [GA]G^G[ACGT]CC[CT]
EagI 325 CGGCCG C^GGCCG
Eam1105I 1105 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 325 CGGCCG C^GGCCG
Eco47III 1233 AGCGCT AGC^GCT
Eco52I 325 CGGCCG C^GGCCG
EcoNI 1290 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 55 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 942 GGTGACC G^GT[ACGT]ACC
EcoRV 331 GATATC GAT^ATC
EcoT22I 1 ATGCAT ATGCA^T
Hin1I 660 GGCGCC G[GA]^CG[CT]C
KasI 660 GGCGCC G^GCGCC
KspI 128 CCGCGG CCGC^GG
MaeI 1246 CTAG C^TAG
NaeI 1500 GCCGGC GCC^GGC
NarI 660 GGCGCC GG^CGCC
NgoMIV 1500 GCCGGC G^CCGGC
NsiI 1 ATGCAT ATGCA^T
PstI 538 CTGCAG CTGCA^G
SacII 128 CCGCGG CCGC^GG
SapI 1446 GCTCTTC GCTCTTC(1/4)
SfoI 660 GGCGCC GGC^GCC
XcmI 638 CCAAGATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 129 CGCG CG^CG
272 CGCG
AfaI 821 GTAC GT^AC
921 GTAC
AocI 589 CCTGAGG CC^T[ACGT]AGG
1041 CCTGAGG
AspI 355 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1417 GACCGTGTC
AvaI 1019 CCCGAG C^[CT]CG[GA]G
1134 CTCGGG
BbsI 361 GTCTTC GAAGAC(2/6)
856 GTCTTC
BbvCI 297 GCTGAGG CCTCAGC(-5/-2)
1062 GCTGAGG
BpuAI 361 GTCTTC GAAGAC(2/6)
856 GTCTTC
BsoBI 1019 CCCGAG C^[CT]CG[GA]G
1134 CTCGGG
BsrDI 1341 CATTGC GCAATG(2/0)
383 GCAATG
BstUI 129 CGCG CG^CG
272 CGCG
BstYI 267 GGATCC [GA]^GATC[CT]
1521 AGATCC
Bsu36I 589 CCTGAGG CC^T[ACGT]AGG
1041 CCTGAGG
Eco81I 589 CCTGAGG CC^T[ACGT]AGG
1041 CCTGAGG
FauI 127 CCCGC CCCGC(4/6)
1080 CCCGC
MflI 267 GGATCC [GA]^GATC[CT]
1521 AGATCC
MvnI 129 CGCG CG^CG
272 CGCG
NcoI 314 CCATGG C^CATGG
1013 CCATGG
PflFI 355 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1417 GACCGTGTC
RsaI 821 GTAC GT^AC
921 GTAC
SfcI 538 CTGCAG C^T[GA][CT]AG
1120 CTACAG
Tth111I 355 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1417 GACCGTGTC
XhoII 267 GGATCC [GA]^GATC[CT]
1521 AGATCC
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BalI BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PvuII
Sau3AI Sau96I ScrFI SfaNI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I