Restriction map for
pF1KE1780
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BpmI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI Ksp632I
KspI MaeII MamI MfeI MluI
MroI MseI MunI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 200 GGTACC G^GTACC
AcyI 15 GGCGCC G[GA]^CG[CT]C
AfaI 201 GTAC GT^AC
AlwI 6 GATCC GGATC(4/5)
AocI 835 CCTGAGG CC^T[ACGT]AGG
ApaI 335 GGGCCC GGGCC^C
Asp718I 200 GGTACC G^GTACC
AspHI 421 GAGCTC G[AT]GC[AT]^C
BalI 22 TGGCCA TGG^CCA
BbeI 15 GGCGCC GGCGC^C
BbsI 696 GAAGAC GAAGAC(2/6)
BbvCI 638 CCTCAGC CCTCAGC(-5/-2)
BlpI 797 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 797 GCTCAGC GC^T[ACGT]AGC
BpuAI 696 GAAGAC GAAGAC(2/6)
BsaHI 15 GGCGCC G[GA]^CG[CT]C
BsiHKAI 421 GAGCTC G[AT]GC[AT]^C
BspMI 157 ACCTGC ACCTGC(4/8)
BsrDI 344 GCAATG GCAATG(2/0)
BstEII 129 GGTGACC G^GT[ACGT]ACC
BstPI 129 GGTGACC G^GT[ACGT]ACC
BstYI 5 AGATCC [GA]^GATC[CT]
Bsu36I 835 CCTGAGG CC^T[ACGT]AGG
CelII 797 GCTCAGC GC^T[ACGT]AGC
Eco81I 835 CCTGAGG CC^T[ACGT]AGG
EcoO65I 129 GGTGACC G^GT[ACGT]ACC
EcoT14I 389 CCAAGG C^C[AT][AT]GG
HaeII 15 GGCGCC [GA]GCGC^[CT]
Hin1I 15 GGCGCC G[GA]^CG[CT]C
HinfI 76 GACTC G^A[ACGT]TC
KasI 15 GGCGCC G^GCGCC
KpnI 200 GGTACC GGTAC^C
MboII 696 GAAGA GAAGA(8/7)
MflI 5 AGATCC [GA]^GATC[CT]
MluNI 22 TGGCCA TGG^CCA
MscI 22 TGGCCA TGG^CCA
MslI 321 CATGAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 487 GCCGGC GCC^GGC
NarI 15 GGCGCC GG^CGCC
NgoMIV 487 GCCGGC G^CCGGC
PflMI 498 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 76 GACTC GAGTC(4/5)
PstI 159 CTGCAG CTGCA^G
PvuII 800 CAGCTG CAG^CTG
RsaI 201 GTAC GT^AC
SacI 421 GAGCTC GAGCT^C
SfcI 159 CTGCAG C^T[GA][CT]AG
SfoI 15 GGCGCC GGC^GCC
SmaI 578 CCCGGG CCC^GGG
StuI 832 AGGCCT AGG^CCT
StyI 389 CCAAGG C^C[AT][AT]GG
Van91I 498 CCAAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 5 AGATCC [GA]^GATC[CT]
XmaCI 578 CCCGGG C^CCGGG
XmaI 578 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AvaI 247 CCCGAG C^[CT]CG[GA]G
578 CCCGGG
BfaI 550 CTAG C^TAG
761 CTAG
BsoBI 247 CCCGAG C^[CT]CG[GA]G
578 CCCGGG
BsrFI 487 GCCGGC [GA]^CCGG[CT]
613 ACCGGC
BssSI 587 CACGAG CACGAG(-5/-1)
124 CTCGTG
BstXI 25 CCAGTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
374 CCATCTCCCTGG
Cfr10I 487 GCCGGC [GA]^CCGG[CT]
613 ACCGGC
DpnII 6 GATC ^GATC
399 GATC
EcoNI 473 CCTCACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
624 CCTGAAGGAGG
MaeI 550 CTAG C^TAG
761 CTAG
MboI 6 GATC ^GATC
399 GATC
MspA1I 351 CAGCGG C[AC]G^C[GT]G
800 CAGCTG
NdeII 6 GATC ^GATC
399 GATC
NlaIII 207 CATG CATG^
321 CATG
PpuMI 557 GGGTCCT [GA]G^G[AT]CC[CT]
632 AGGACCC
Sau3AI 6 GATC ^GATC
399 GATC
XcmI 10 CCACAGGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
82 CCAGACAGGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvI BcnI BglI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MnlI MspI
MvaI MwoI NciI NlaIV Sau96I
ScrFI TseI Tsp45I TspRI