Restriction map for
pF1KB4823
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI AlwNI AocI Aor51HI
ApoI AscI Asp700I Asp718I AvrII
BbeI BbrPI BciVI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoRV EcoT22I FseI HaeII HpaI
KasI KpnI KspI MamI MfeI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tsp509I Van91I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AcyI 909 GACGCC G[GA]^CG[CT]C
AflII 1470 CTTAAG C^TTAAG
AhdI 67 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1132 ATTAAT AT^TAAT
AsnI 1132 ATTAAT AT^TAAT
AspEI 67 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 864 TGCGCA TGC^GCA
BamHI 1708 GGATCC G^GATCC
BanI 636 GGTGCC G^G[CT][GA]CC
BbsI 941 GTCTTC GAAGAC(2/6)
BbvCI 615 CCTCAGC CCTCAGC(-5/-2)
BcgI 1589 CGATTCCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1257 TGATCA T^GATCA
BfaI 649 CTAG C^TAG
BfrI 1470 CTTAAG C^TTAAG
BlpI 1716 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1716 GCTCAGC GC^T[ACGT]AGC
BpuAI 941 GTCTTC GAAGAC(2/6)
BsaAI 571 TACGTG [CT]AC^GT[GA]
BsaHI 909 GACGCC G[GA]^CG[CT]C
BsaI 1397 GGTCTC GGTCTC(1/5)
BsgI 1423 GTGCAG GTGCAG(16/14)
Bsp1407I 1331 TGTACA T^GTACA
BspHI 1067 TCATGA T^CATGA
BsrGI 1331 TGTACA T^GTACA
BstEII 1006 GGTGACC G^GT[ACGT]ACC
BstPI 1006 GGTGACC G^GT[ACGT]ACC
BstXI 1497 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1716 GCTCAGC GC^T[ACGT]AGC
Eam1105I 67 GACAACGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 526 CTCTTC CTCTTC(1/4)
EcoNI 162 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1006 GGTGACC G^GT[ACGT]ACC
FbaI 1257 TGATCA T^GATCA
FspI 864 TGCGCA TGC^GCA
Hin1I 909 GACGCC G[GA]^CG[CT]C
HindIII 844 AAGCTT A^AGCTT
Ksp632I 526 CTCTTC CTCTTC(1/4)
MaeI 649 CTAG C^TAG
MluI 2009 ACGCGT A^CGCGT
NcoI 461 CCATGG C^CATGG
PaeR7I 1746 CTCGAG C^TCGAG
PshBI 1132 ATTAAT AT^TAAT
RcaI 1067 TCATGA T^CATGA
SapI 525 GCTCTTC GCTCTTC(1/4)
SfiI 450 GGCCCACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 857 CCCGGG CCC^GGG
SspBI 1331 TGTACA T^GTACA
StuI 410 AGGCCT AGG^CCT
XhoI 1746 CTCGAG C^TCGAG
XmaCI 857 CCCGGG C^CCGGG
XmaI 857 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 310 GTGCAC G^TGCAC
2035 GTGCAC
ApaI 223 GGGCCC GGGCC^C
304 GGGCCC
ApaLI 310 GTGCAC G^TGCAC
2035 GTGCAC
AspHI 310 GTGCAC G[AT]GC[AT]^C
2035 GTGCAC
AspI 471 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
973 GACGCTGTC
BsiHKAI 310 GTGCAC G[AT]GC[AT]^C
2035 GTGCAC
BsmAI 979 GTCTC GTCTC(1/5)
1398 GTCTC
BspMI 1826 ACCTGC ACCTGC(4/8)
960 GCAGGT
BsrBI 762 GAGCGG CCGCTC(-3/-3)
1645 GAGCGG
BsrDI 176 GCAATG GCAATG(2/0)
1874 GCAATG
DsaI 461 CCATGG C^C[GA][CT]GG
1773 CCACGG
HincII 481 GTCAAC GT[CT]^[GA]AC
1918 GTCAAC
HindII 481 GTCAAC GT[CT]^[GA]AC
1918 GTCAAC
MaeIII 1007 GTGAC ^GT[ACGT]AC
1670 GTGAC
PflFI 471 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
973 GACGCTGTC
PstI 1447 CTGCAG CTGCA^G
1665 CTGCAG
PvuII 253 CAGCTG CAG^CTG
946 CAGCTG
TaqI 1455 TCGA T^CGA
1747 TCGA
Tsp45I 1007 GTGAC ^GT[GC]AC
1670 GTGAC
Tth111I 471 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
973 GACGCTGTC
TthHB8I 1455 TCGA T^CGA
1747 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AvaI AvaII BalI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI TspRI XhoII