Restriction map for
pF1KE2008
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspEI BspHI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AlwNI 18 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1393 ATTAAT AT^TAAT
AsnI 1393 ATTAAT AT^TAAT
Asp700I 1312 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 684 TGGCCA TGG^CCA
BanI 369 GGCACC G^G[CT][GA]CC
BsaI 1603 GGTCTC GGTCTC(1/5)
BseRI 1606 CTCCTC GAGGAG(10/8)
BsgI 232 GTGCAG GTGCAG(16/14)
BsmI 1080 GAATGC GAATGC(1/-1)
Bsp1407I 1646 TGTACA T^GTACA
BspLU11I 1677 ACATGT A^CATGT
BsrBI 545 GAGCGG CCGCTC(-3/-3)
BsrDI 914 GCAATG GCAATG(2/0)
BsrGI 1646 TGTACA T^GTACA
Bst1107I 84 GTATAC GTA^TAC
BstYI 211 AGATCC [GA]^GATC[CT]
BstZ17I 84 GTATAC GTA^TAC
CfoI 912 GCGC GCG^C
DraI 562 TTTAAA TTT^AAA
DraII 1688 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 684 TGGCCA [CT]^GGCC[GA]
EcoO109I 1688 AGGACCT [GA]G^G[ACGT]CC[CT]
HgaI 1361 GCGTC GACGC(5/10)
HhaI 912 GCGC GCG^C
HinP1I 912 GCGC G^CGC
MflI 211 AGATCC [GA]^GATC[CT]
MluNI 684 TGGCCA TGG^CCA
MscI 684 TGGCCA TGG^CCA
MslI 639 CATACAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 101 CATATG CA^TATG
NheI 1072 GCTAGC G^CTAGC
PpuMI 1688 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 1393 ATTAAT AT^TAAT
PstI 1186 CTGCAG CTGCA^G
SapI 33 GCTCTTC GCTCTTC(1/4)
SphI 338 GCATGC GCATG^C
SspBI 1646 TGTACA T^GTACA
StuI 72 AGGCCT AGG^CCT
XhoII 211 AGATCC [GA]^GATC[CT]
XmnI 1312 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 84 GTATAC GT^[AC][GT]AC
1636 GTCTAC
AciI 53 CCGC CCGC(-3/-1)
547 GCGG
AcsI 93 AAATTC [GA]^AATT[CT]
838 GAATTT
AflIII 359 ACGTGT A^C[GA][CT]GT
1677 ACATGT
ApoI 93 AAATTC [GA]^AATT[CT]
838 GAATTT
BpmI 613 CTGGAG CTGGAG(16/14)
1471 CTGGAG
EarI 34 CTCTTC CTCTTC(1/4)
1432 GAAGAG
Ksp632I 34 CTCTTC CTCTTC(1/4)
1432 GAAGAG
MaeII 137 ACGT A^CGT
359 ACGT
NspI 338 GCATGC [GA]CATG^[CT]
1677 ACATGT
PflMI 70 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1331 CCATTCAATGG
SfcI 734 CTACAG C^T[GA][CT]AG
1186 CTGCAG
TaqI 437 TCGA T^CGA
1119 TCGA
TthHB8I 437 TCGA T^CGA
1119 TCGA
Van91I 70 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1331 CCATTCAATGG
XcmI 70 CCAGGCCTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1327 CCAGCCATTCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI AlwI AvaII BbsI
BbvI BfaI BpuAI BsaJI BsiYI
BslI BsmAI BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI