##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmI        BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DdeI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        Fnu4HI      
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HinfI       
HpaII       ItaI        KasI        KpnI        KspI        
MaeI        MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
StyI        SwaI        TaqI        TfiI        TseI        
Tsp45I      Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AfaI          236  GTAC                 GT^AC
AlwI          510  GATCC                GGATC(4/5)
AvaII         369  GGACC                G^G[AT]CC
BanII          79  GAGCTC               G[GA]GC[CT]^C
BglII         153  AGATCT               A^GATCT
BsgI          329  CTGCAC               GTGCAG(16/14)
BsmAI         385  GAGAC                GTCTC(1/5)
BsmBI         385  GAGACG               CGTCTC(1/5)
BsmFI         412  GTCCC                GGGAC(10/14)
Bsp1407I      235  TGTACA               T^GTACA
BsrGI         235  TGTACA               T^GTACA
BssKI         570  CCAGG                ^CC[ACGT]GG
BstNI         570  CCAGG                CC^[AT]GG
BstYI         153  AGATCT               [GA]^GATC[CT]
Cac8I         265  GCTGGC               GC[ACGT]^[ACGT]GC
DraI           37  TTTAAA               TTT^AAA
EcoRI         574  GAATTC               G^AATTC
EcoRII        570  CCAGG                ^CC[AT]GG
HaeIII        268  GGCC                 GG^CC
HincII        175  GTTAAC               GT[CT]^[GA]AC
HindII        175  GTTAAC               GT[CT]^[GA]AC
HpaI          175  GTTAAC               GTT^AAC
MaeII         388  ACGT                 A^CGT
MflI          153  AGATCT               [GA]^GATC[CT]
MspA1I        263  CAGCTG               C[AC]G^C[GT]G
MvaI          570  CCAGG                CC^[AT]GG
NspI          206  ACATGC               [GA]CATG^[CT]
PvuII         263  CAGCTG               CAG^CTG
RsaI          236  GTAC                 GT^AC
SacI           79  GAGCTC               GAGCT^C
SapI           23  GCTCTTC              GCTCTTC(1/4)
ScrFI         570  CCAGG                CC^[ACGT]GG
SspBI         235  TGTACA               T^GTACA
XhoII         153  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AcsI          426  GAATTT               [GA]^AATT[CT]
              574  GAATTC              
ApoI          426  GAATTT               [GA]^AATT[CT]
              574  GAATTC              
AspHI          79  GAGCTC               G[AT]GC[AT]^C
              324  GTGCTC              
BmyI           79  GAGCTC               G[AGT]GC[ACT]^C
              324  GTGCTC              
BsiHKAI        79  GAGCTC               G[AT]GC[AT]^C
              324  GTGCTC              
Bsp1286I       79  GAGCTC               G[AGT]GC[ACT]^C
              324  GTGCTC              
BstF5I        125  GGATG                GGATG(2/0)
              378  GGATG               
Cfr13I        268  GGCCC                G^G[ACGT]CC
              369  GGACC               
FokI          125  GGATG                GGATG(9/13)
              378  GGATG               
HphI           69  TCACC                GGTGA(8/7)
              632  TCACC               
MslI           70  CACCAGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              432  CATGTCTGTG          
Sau96I        268  GGCCC                G^G[ACGT]CC
              369  GGACC               


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AluI        BsiYI       BslI        BsrI        DpnII       
EarI        Ksp632I     MboI        MboII       MnlI        
MseI        NdeII       NlaIII      Sau3AI      Tru9I       
Tsp509I     TspRI