Restriction map for
pF1KE2357
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AocI Aor51HI ApaI AscI AspI
AviII AvrII BbeI BbrPI BcnI
BglI BlnI BlpI Bpu1102I BsaHI
BseAI BsiEI BsiWI BspEI BsrBI
BssHII BstBI BstEII BstPI BstUI
Bsu36I CelII CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FseI FspI Hin1I HpaI
KasI KspI MluI MroI MvnI
NaeI NarI NciI NcoI NgoMIV
NotI NruI NspV PacI PflFI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacI SacII SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AflII 229 CTTAAG C^TTAAG
AgeI 2250 ACCGGT A^CCGGT
Alw44I 2447 GTGCAC G^TGCAC
ApaLI 2447 GTGCAC G^TGCAC
AvaI 1004 CTCGAG C^[CT]CG[GA]G
BcgI 5253 GCACAAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 229 CTTAAG C^TTAAG
BsaAI 2042 TACGTG [CT]AC^GT[GA]
BsaWI 2250 ACCGGT [AT]^CCGG[AT]
BsmBI 2662 GAGACG CGTCTC(1/5)
BsoBI 1004 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3857 TGTACA T^GTACA
BspDI 5261 ATCGAT AT^CGAT
BspMI 4750 GCAGGT ACCTGC(4/8)
BsrGI 3857 TGTACA T^GTACA
BssSI 5485 CTCGTG CACGAG(-5/-1)
ClaI 5261 ATCGAT AT^CGAT
EcoT22I 2749 ATGCAT ATGCA^T
FauI 1771 CCCGC CCCGC(4/6)
MfeI 3953 CAATTG C^AATTG
MunI 3953 CAATTG C^AATTG
NheI 5304 GCTAGC G^CTAGC
NsiI 2749 ATGCAT ATGCA^T
PaeR7I 1004 CTCGAG C^TCGAG
PinAI 2250 ACCGGT A^CCGGT
PshAI 4495 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1216 GTCGAC G^TCGAC
SexAI 6755 ACCAGGT A^CC[AT]GGT
SspBI 3857 TGTACA T^GTACA
XbaI 877 TCTAGA T^CTAGA
XhoI 1004 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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Acc65I 3846 GGTACC G^GTACC
4660 GGTACC
AhdI 1001 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5663 GACGTGTGGTC
AseI 310 ATTAAT AT^TAAT
1260 ATTAAT
AsnI 310 ATTAAT AT^TAAT
1260 ATTAAT
Asp718I 3846 GGTACC G^GTACC
4660 GGTACC
AspEI 1001 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5663 GACGTGTGGTC
BamHI 1932 GGATCC G^GATCC
4739 GGATCC
BciVI 70 GGATAC GTATCC(6/5)
4468 GTATCC
BsaI 3511 GAGACC GGTCTC(1/5)
2211 GGTCTC
BsmI 3243 GAATGC GAATGC(1/-1)
29 GCATTC
BspLU11I 163 ACATGT A^CATGT
3218 ACATGT
BsrFI 2250 ACCGGT [GA]^CCGG[CT]
4228 GCCGGT
Bst1107I 2230 GTATAC GTA^TAC
2254 GTATAC
BstXI 4143 CCACAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6875 CCACCTCTCTGG
BstZ17I 2230 GTATAC GTA^TAC
2254 GTATAC
Cfr10I 2250 ACCGGT [GA]^CCGG[CT]
4228 GCCGGT
DrdI 325 GACTACCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2064 GACAACCAAGTC
DsaI 2216 CCACGG C^C[GA][CT]GG
6743 CCGTGG
Eam1105I 1001 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5663 GACGTGTGGTC
HapII 2251 CCGG C^CGG
4229 CCGG
HgaI 2664 GACGC GACGC(5/10)
3247 GCGTC
HpaII 2251 CCGG C^CGG
4229 CCGG
KpnI 3846 GGTACC GGTAC^C
4660 GGTACC
MspI 2251 CCGG C^CGG
4229 CCGG
PpuMI 2103 GGGTCCC [GA]G^G[AT]CC[CT]
3301 GGGTCCC
PshBI 310 ATTAAT AT^TAAT
1260 ATTAAT
SspI 193 AATATT AAT^ATT
3618 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI