Restriction map for
pF1KE2360
##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccII AflII AgeI AhdI
AscI AspEI AspI AvrII BamHI
BbeI BbvCI BcgI BclI BfrI
BlnI BsiEI BsiWI Bsp1407I BspDI
BsrFI BsrGI BssHII BssSI BstEII
BstPI BstUI BstXI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FbaI FseI
HpaI KasI KspI MluI MvnI
NaeI NarI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PvuI RsrII SacII
SalI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SspBI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2277 GGTACC G^GTACC
AccIII 936 TCCGGA T^CCGGA
AcyI 670 GACGCC G[GA]^CG[CT]C
Aor51HI 3095 AGCGCT AGC^GCT
ApaI 284 GGGCCC GGGCC^C
Asp718I 2277 GGTACC G^GTACC
AviII 11 TGCGCA TGC^GCA
BanI 2277 GGTACC G^G[CT][GA]CC
BbrPI 337 CACGTG CAC^GTG
BglI 1319 GCCCAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 670 GACGCC G[GA]^CG[CT]C
BsaI 402 GAGACC GGTCTC(1/5)
BsaWI 936 TCCGGA [AT]^CCGG[AT]
BseAI 936 TCCGGA T^CCGGA
BsmFI 371 GGGAC GGGAC(10/14)
BspEI 936 TCCGGA T^CCGGA
BspHI 2588 TCATGA T^CATGA
BsrBI 6004 GAGCGG CCGCTC(-3/-3)
Bst1107I 4542 GTATAC GTA^TAC
BstZ17I 4542 GTATAC GTA^TAC
Eco47III 3095 AGCGCT AGC^GCT
EcoNI 4559 CCTTTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2005 GAATTC G^AATTC
FspI 11 TGCGCA TGC^GCA
HaeII 3095 AGCGCT [GA]GCGC^[CT]
Hin1I 670 GACGCC G[GA]^CG[CT]C
HincII 236 GTCAAC GT[CT]^[GA]AC
HindII 236 GTCAAC GT[CT]^[GA]AC
KpnI 2277 GGTACC GGTAC^C
MroI 936 TCCGGA T^CCGGA
PflMI 747 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 337 CACGTG CAC^GTG
PmlI 337 CACGTG CAC^GTG
RcaI 2588 TCATGA T^CATGA
SbfI 2062 CCTGCAGG CCTGCA^GG
Sse8387I 2062 CCTGCAGG CCTGCA^GG
Van91I 747 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2918 CCACATACTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AclI 1094 AACGTT AA^CGTT
5129 AACGTT
Alw44I 1525 GTGCAC G^TGCAC
2482 GTGCAC
ApaLI 1525 GTGCAC G^TGCAC
2482 GTGCAC
AseI 4293 ATTAAT AT^TAAT
5728 ATTAAT
AsnI 4293 ATTAAT AT^TAAT
5728 ATTAAT
AvaI 2954 CCCGAG C^[CT]CG[GA]G
2971 CCCGAG
AvaII 1162 GGACC G^G[AT]CC
5872 GGTCC
BciVI 3802 GTATCC GTATCC(6/5)
5922 GTATCC
BcnI 2010 CCGGG CC^[GC]GG
4094 CCCGG
BglII 186 AGATCT A^GATCT
2472 AGATCT
BsaAI 337 CACGTG [CT]AC^GT[GA]
5689 TACGTG
BsmBI 567 GAGACG CGTCTC(1/5)
850 GAGACG
BsmI 1571 GAATGC GAATGC(1/-1)
6123 GCATTC
BsoBI 2954 CCCGAG C^[CT]CG[GA]G
2971 CCCGAG
BstAPI 2316 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4050 GCAAACCATGC
BstBI 377 TTCGAA TT^CGAA
4067 TTCGAA
DraII 283 AGGGCCC [GA]G^G[ACGT]CC[CT]
5597 AGGCCCC
DraIII 1469 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
4245 CACTCAGTG
EcoO109I 283 AGGGCCC [GA]G^G[ACGT]CC[CT]
5597 AGGCCCC
HgaI 670 GACGC GACGC(5/10)
2029 GACGC
NciI 2010 CCGGG CC^[GC]GG
4094 CCCGG
NcoI 2573 CCATGG C^CATGG
2678 CCATGG
NdeI 1811 CATATG CA^TATG
5882 CATATG
NheI 3082 GCTAGC G^CTAGC
5914 GCTAGC
NspV 377 TTCGAA TT^CGAA
4067 TTCGAA
PshBI 4293 ATTAAT AT^TAAT
5728 ATTAAT
Psp1406I 1094 AACGTT AA^CGTT
5129 AACGTT
ScaI 2876 AGTACT AGT^ACT
4221 AGTACT
SfuI 377 TTCGAA TT^CGAA
4067 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI BalI BanII BbsI
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
Eco81I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MfeI MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MunI MvaI MwoI
NdeII NlaIII NlaIV NsiI NspI
PleI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI