Restriction map for
pF1KE1597
##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccII AccIII AflII AgeI AhdI
ApaI AscI AspEI AviII BbeI
BbrPI BfrI BlpI Bpu1102I BsaBI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrBI BssHII Bst1107I BstUI
BstZ17I CelII ClaI CpoI EagI
Eam1105I EclXI Eco52I FseI FspI
HpaI KasI KspI MamI MluI
MroI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PvuI RsrII SacI SacII SalI
SbfI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 631 GACGTC GACGT^C
AclI 7555 AACGTT AA^CGTT
Alw44I 8349 GTGCAC G^TGCAC
Aor51HI 8229 AGCGCT AGC^GCT
ApaLI 8349 GTGCAC G^TGCAC
AseI 5695 ATTAAT AT^TAAT
AsnI 5695 ATTAAT AT^TAAT
AvaI 3863 CCCGGG C^[CT]CG[GA]G
BamHI 6779 GGATCC G^GATCC
BanII 8637 GAGCCC G[GA]GC[CT]^C
BcgI 6694 GCATCGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 1739 CACGTA [CT]AC^GT[GA]
BsaWI 8245 ACCGGA [AT]^CCGG[AT]
BsoBI 3863 CCCGGG C^[CT]CG[GA]G
BspLU11I 8498 ACATGT A^CATGT
BsrFI 9047 GCCGGT [GA]^CCGG[CT]
BssSI 4146 CACGAG CACGAG(-5/-1)
BstAPI 8866 GCATTTTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 8312 TTCGAA TT^CGAA
BstEII 3090 GGTTACC G^GT[ACGT]ACC
BstPI 3090 GGTTACC G^GT[ACGT]ACC
Cfr10I 9047 GCCGGT [GA]^CCGG[CT]
Eco47III 8229 AGCGCT AGC^GCT
EcoO65I 3090 GGTTACC G^GT[ACGT]ACC
HaeII 8229 AGCGCT [GA]GCGC^[CT]
NspV 8312 TTCGAA TT^CGAA
PshBI 5695 ATTAAT AT^TAAT
Psp1406I 7555 AACGTT AA^CGTT
ScaI 5524 AGTACT AGT^ACT
SexAI 8372 ACCTGGT A^CC[AT]GGT
SfuI 8312 TTCGAA TT^CGAA
SmaI 3863 CCCGGG CCC^GGG
SphI 169 GCATGC GCATG^C
XmaCI 3863 CCCGGG C^CCGGG
XmaI 3863 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2137 GGTACC G^GTACC
3189 GGTACC
AcyI 631 GACGTC G[GA]^CG[CT]C
9114 GACGCC
AocI 4336 CCTGAGG CC^T[ACGT]AGG
6313 CCTGAGG
Asp700I 1087 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8892 GAACTTTTTC
Asp718I 2137 GGTACC G^GTACC
3189 GGTACC
AspI 6354 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6387 GACTATGTC
BclI 8339 TGATCA T^GATCA
8384 TGATCA
BglII 5537 AGATCT A^GATCT
8619 AGATCT
BsaHI 631 GACGTC G[GA]^CG[CT]C
9114 GACGCC
BspHI 2058 TCATGA T^CATGA
8720 TCATGA
Bsu36I 4336 CCTGAGG CC^T[ACGT]AGG
6313 CCTGAGG
DraI 2260 TTTAAA TTT^AAA
7425 TTTAAA
DrdI 2955 GACAGCTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3822 GACAGAAATGTC
Eco81I 4336 CCTGAGG CC^T[ACGT]AGG
6313 CCTGAGG
FauI 7707 GCGGG CCCGC(4/6)
8979 GCGGG
FbaI 8339 TGATCA T^GATCA
8384 TGATCA
HgaI 9114 GACGC GACGC(5/10)
3466 GCGTC
Hin1I 631 GACGTC G[GA]^CG[CT]C
9114 GACGCC
HindIII 25 AAGCTT A^AGCTT
9192 AAGCTT
KpnI 2137 GGTACC GGTAC^C
3189 GGTACC
NspI 169 GCATGC [GA]CATG^[CT]
8498 ACATGT
PflFI 6354 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6387 GACTATGTC
PflMI 567 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3052 CCAGTTAATGG
PstI 8011 CTGCAG CTGCA^G
8435 CTGCAG
RcaI 2058 TCATGA T^CATGA
8720 TCATGA
SapI 6769 GAAGAGC GCTCTTC(1/4)
7010 GCTCTTC
Tth111I 6354 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
6387 GACTATGTC
Van91I 567 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3052 CCAGTTAATGG
XmnI 1087 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
8892 GAACTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII AvrII BalI BanI BbsI
BbvCI BbvI BciVI BcnI BfaI
BglI BlnI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NsiI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII