Restriction map for
pF1KE2383
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BbrPI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 124 GTCTAC GT^[AC][GT]AC
AccII 1134 CGCG CG^CG
Aor51HI 1177 AGCGCT AGC^GCT
AseI 988 ATTAAT AT^TAAT
AsnI 988 ATTAAT AT^TAAT
AvaI 940 CTCGGG C^[CT]CG[GA]G
BalI 393 TGGCCA TGG^CCA
BamHI 413 GGATCC G^GATCC
BanII 866 GAGCCC G[GA]GC[CT]^C
BbvCI 812 CCTCAGC CCTCAGC(-5/-2)
BfaI 997 CTAG C^TAG
BlpI 604 GCTCAGC GC^T[ACGT]AGC
BmyI 866 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 604 GCTCAGC GC^T[ACGT]AGC
BsaI 1250 GAGACC GGTCTC(1/5)
BsaWI 149 ACCGGA [AT]^CCGG[AT]
BseRI 702 CTCCTC GAGGAG(10/8)
BsoBI 940 CTCGGG C^[CT]CG[GA]G
Bsp1286I 866 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 1148 TGTACA T^GTACA
BspMI 285 ACCTGC ACCTGC(4/8)
BsrDI 475 GCAATG GCAATG(2/0)
BsrGI 1148 TGTACA T^GTACA
BstUI 1134 CGCG CG^CG
CelII 604 GCTCAGC GC^T[ACGT]AGC
DraII 423 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1360 GACAATCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 393 TGGCCA [CT]^GGCC[GA]
EarI 531 CTCTTC CTCTTC(1/4)
Eco47III 1177 AGCGCT AGC^GCT
EcoO109I 423 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1112 GAATTC G^AATTC
Ksp632I 531 CTCTTC CTCTTC(1/4)
MaeI 997 CTAG C^TAG
MaeII 137 ACGT A^CGT
MluNI 393 TGGCCA TGG^CCA
MscI 393 TGGCCA TGG^CCA
MvnI 1134 CGCG CG^CG
PpuMI 423 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 148 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 988 ATTAAT AT^TAAT
SapI 530 GCTCTTC GCTCTTC(1/4)
SmlI 217 CTTGAG C^T[CT][GA]AG
SspBI 1148 TGTACA T^GTACA
StuI 1100 AGGCCT AGG^CCT
Tsp45I 457 GTCAC ^GT[GC]AC
XcmI 1389 CCAAGCTATGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 1112 GAATTC [GA]^AATT[CT]
1243 GAATTT
AfaI 1122 GTAC GT^AC
1149 GTAC
ApoI 1112 GAATTC [GA]^AATT[CT]
1243 GAATTT
BbeI 584 GGCGCC GGCGC^C
1048 GGCGCC
BbsI 316 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BpuAI 316 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BsrI 957 ACTGG ACTGG(1/-1)
1366 CCAGT
BssSI 849 CACGAG CACGAG(-5/-1)
873 CACGAG
BstAPI 481 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1375 GCACAAGCTGC
BstF5I 268 CATCC GGATG(2/0)
260 GGATG
BstXI 305 CCAACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
707 CCAGCAACCTGG
BstYI 65 AGATCC [GA]^GATC[CT]
413 GGATCC
EcoT14I 518 CCATGG C^C[AT][AT]GG
1082 CCATGG
EcoT22I 262 ATGCAT ATGCA^T
502 ATGCAT
FokI 268 CATCC GGATG(9/13)
260 GGATG
HgaI 780 GACGC GACGC(5/10)
1087 GACGC
KasI 584 GGCGCC G^GCGCC
1048 GGCGCC
MaeIII 190 GTAAC ^GT[ACGT]AC
457 GTCAC
MflI 65 AGATCC [GA]^GATC[CT]
413 GGATCC
NarI 584 GGCGCC GG^CGCC
1048 GGCGCC
NcoI 518 CCATGG C^CATGG
1082 CCATGG
NsiI 262 ATGCAT ATGCA^T
502 ATGCAT
PvuII 797 CAGCTG CAG^CTG
1435 CAGCTG
RsaI 1122 GTAC GT^AC
1149 GTAC
SfoI 584 GGCGCC GGC^GCC
1048 GGCGCC
SspI 241 AATATT AAT^ATT
985 AATATT
StyI 518 CCATGG C^C[AT][AT]GG
1082 CCATGG
TaqI 346 TCGA T^CGA
441 TCGA
TthHB8I 346 TCGA T^CGA
441 TCGA
XhoII 65 AGATCC [GA]^GATC[CT]
413 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AvaII BanI BbvI BcnI BpmI
BsaHI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoNI EcoRII Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI Tru9I
TseI Tsp509I TspRI