################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## Acc65I AccIII AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiHKAI BsiWI BsoBI BspDI BspEI BsrBI BsrFI BssHII Bst1107I BstBI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I FauI FbaI FseI FspI HpaI KasI KpnI KspI MluI MroI NaeI NarI NciI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1483 GACGTC GACGT^C AccII 2525 CGCG CG^CG AciI 772 GCGG CCGC(-3/-1) AclI 1592 AACGTT AA^CGTT AcyI 1483 GACGTC G[GA]^CG[CT]C AflII 2164 CTTAAG C^TTAAG AocI 5509 CCTCAGG CC^T[ACGT]AGG Aor51HI 1847 AGCGCT AGC^GCT BamHI 1309 GGATCC G^GATCC BanI 4057 GGTGCC G^G[CT][GA]CC BcgI 589 CGAAAGATTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4647 GGATAC GTATCC(6/5) BfrI 2164 CTTAAG C^TTAAG BsaHI 1483 GACGTC G[GA]^CG[CT]C BsaI 4618 GGTCTC GGTCTC(1/5) BsaWI 4554 ACCGGA [AT]^CCGG[AT] BsgI 1190 CTGCAC GTGCAG(16/14) BsmBI 1481 GAGACG CGTCTC(1/5) BspLU11I 546 ACATGT A^CATGT BsrDI 1263 CATTGC GCAATG(2/0) BssSI 3794 CACGAG CACGAG(-5/-1) BstEII 202 GGTGACC G^GT[ACGT]ACC BstPI 202 GGTGACC G^GT[ACGT]ACC BstUI 2525 CGCG CG^CG BstXI 3812 CCAAACACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 5509 CCTCAGG CC^T[ACGT]AGG CfoI 1848 GCGC GCG^C Eco47III 1847 AGCGCT AGC^GCT Eco81I 5509 CCTCAGG CC^T[ACGT]AGG EcoO65I 202 GGTGACC G^GT[ACGT]ACC EcoRV 4909 GATATC GAT^ATC HaeII 1847 AGCGCT [GA]GCGC^[CT] HapII 4555 CCGG C^CGG HgaI 3760 GCGTC GACGC(5/10) HhaI 1848 GCGC GCG^C Hin1I 1483 GACGTC G[GA]^CG[CT]C HinP1I 1848 GCGC G^CGC HpaII 4555 CCGG C^CGG MspA1I 4667 CAGCTG C[AC]G^C[GT]G MspI 4555 CCGG C^CGG MvnI 2525 CGCG CG^CG PflMI 4036 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 1592 AACGTT AA^CGTT PvuII 4667 CAGCTG CAG^CTG StuI 4815 AGGCCT AGG^CCT Van91I 4036 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 1822 GTAGAC GT^[AC][GT]AC 3235 GTCTAC AflIII 546 ACATGT A^C[GA][CT]GT 2576 ACACGT AlwNI 1297 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 4037 CAGAGGCTG BanII 258 GAGCCC G[GA]GC[CT]^C 1027 GAGCCC BbsI 4516 GAAGAC GAAGAC(2/6) 4797 GTCTTC BmyI 258 GAGCCC G[AGT]GC[ACT]^C 1027 GAGCCC BpuAI 4516 GAAGAC GAAGAC(2/6) 4797 GTCTTC Bsp1286I 258 GAGCCC G[AGT]GC[ACT]^C 1027 GAGCCC DraI 2443 TTTAAA TTT^AAA 4354 TTTAAA DraII 71 AGGACCC [GA]G^G[ACGT]CC[CT] 2958 AGGACCT EcoNI 1580 CCTATACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4726 CCTCCAAGAGG EcoO109I 71 AGGACCC [GA]G^G[ACGT]CC[CT] 2958 AGGACCT EcoRI 821 GAATTC G^AATTC 3587 GAATTC MfeI 2555 CAATTG C^AATTG 2786 CAATTG MunI 2555 CAATTG C^AATTG 2786 CAATTG PpuMI 71 AGGACCC [GA]G^G[AT]CC[CT] 2958 AGGACCT SapI 3520 GAAGAGC GCTCTTC(1/4) 4871 GAAGAGC SexAI 2009 ACCTGGT A^CC[AT]GGT 4894 ACCTGGT SphI 425 GCATGC GCATG^C 1391 GCATGC XcmI 1409 CCATTCCGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4239 CCACTTGGAAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AseI AsnI Asp700I AvaII BalI BbvI BfaI BglII BpmI BsaBI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1407I BspHI BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HincII HindII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MvaI MwoI NcoI NdeI NdeII NheI NlaIII NlaIV NsiI NspI PleI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI