Restriction map for
pF1KB3789
##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AspI AviII
AvrII BciVI BfrI BlnI BsiWI
Bsp1407I BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DraI EagI
EclXI Eco52I EcoRV EcoT22I FseI
FspI HpaI KpnI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 743 ACACGT A^C[GA][CT]GT
Alw44I 1686 GTGCAC G^TGCAC
AocI 1845 CCTGAGG CC^T[ACGT]AGG
ApaLI 1686 GTGCAC G^TGCAC
BamHI 840 GGATCC G^GATCC
BclI 1226 TGATCA T^GATCA
BsiEI 1256 CGGTCG CG[GA][CT]^CG
BsmBI 2573 GAGACG CGTCTC(1/5)
BspDI 4057 ATCGAT AT^CGAT
BsrDI 3710 CATTGC GCAATG(2/0)
BstAPI 2389 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1845 CCTGAGG CC^T[ACGT]AGG
ClaI 4057 ATCGAT AT^CGAT
DraIII 87 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1845 CCTGAGG CC^T[ACGT]AGG
EcoNI 1955 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3880 GAATTC G^AATTC
FbaI 1226 TGATCA T^GATCA
NaeI 4582 GCCGGC GCC^GGC
NgoMIV 4582 GCCGGC G^CCGGC
NspI 2696 GCATGC [GA]CATG^[CT]
PshAI 1188 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1485 GAAGAGC GCTCTTC(1/4)
SexAI 3212 ACCTGGT A^CC[AT]GGT
SphI 2696 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 2506 GACGTC GACGT^C
4200 GACGTC
AccI 529 GTCTAC GT^[AC][GT]AC
1699 GTCTAC
AccIII 3884 TCCGGA T^CCGGA
4415 TCCGGA
AcsI 1520 AAATTC [GA]^AATT[CT]
3880 GAATTC
Aor51HI 634 AGCGCT AGC^GCT
3515 AGCGCT
ApoI 1520 AAATTC [GA]^AATT[CT]
3880 GAATTC
Asp700I 3219 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4224 GAAGGACTTC
AspHI 1686 GTGCAC G[AT]GC[AT]^C
3028 GAGCAC
BcgI 3620 GCAACTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4064 GCATCAACATCG
BglI 2033 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2068 GCCCTCTTGGC
BglII 1031 AGATCT A^GATCT
2534 AGATCT
BsaBI 4050 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4107 GATCACCATC
BsaI 2827 GAGACC GGTCTC(1/5)
3469 GAGACC
BseAI 3884 TCCGGA T^CCGGA
4415 TCCGGA
BsiHKAI 1686 GTGCAC G[AT]GC[AT]^C
3028 GAGCAC
BspEI 3884 TCCGGA T^CCGGA
4415 TCCGGA
BspHI 977 TCATGA T^CATGA
2519 TCATGA
BssSI 112 CTCGTG CACGAG(-5/-1)
1135 CTCGTG
BstEII 3173 GGTCACC G^GT[ACGT]ACC
3717 GGTCACC
BstPI 3173 GGTCACC G^GT[ACGT]ACC
3717 GGTCACC
Eco47III 634 AGCGCT AGC^GCT
3515 AGCGCT
EcoO65I 3173 GGTCACC G^GT[ACGT]ACC
3717 GGTCACC
HincII 1270 GTCAAC GT[CT]^[GA]AC
3013 GTCAAC
HindII 1270 GTCAAC GT[CT]^[GA]AC
3013 GTCAAC
HindIII 1546 AAGCTT A^AGCTT
2875 AAGCTT
MamI 4050 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4107 GATCACCATC
MroI 3884 TCCGGA T^CCGGA
4415 TCCGGA
NcoI 1799 CCATGG C^CATGG
3101 CCATGG
PaeR7I 2570 CTCGAG C^TCGAG
3427 CTCGAG
PpuMI 3911 AGGACCT [GA]G^G[AT]CC[CT]
4124 AGGACCC
RcaI 977 TCATGA T^CATGA
2519 TCATGA
ScaI 1148 AGTACT AGT^ACT
4178 AGTACT
SmaI 2336 CCCGGG CCC^GGG
4322 CCCGGG
XhoI 2570 CTCGAG C^TCGAG
3427 CTCGAG
XmaCI 2336 CCCGGG C^CCGGG
4322 CCCGGG
XmaI 2336 CCCGGG C^CCGGG
4322 CCCGGG
XmnI 3219 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4224 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI ApaI AspEI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI Eam1105I EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PflMI PleI
PmaCI PmlI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII