################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AclI AflII AgeI AscI AseI AsnI Asp718I AspI AviII AvrII BciVI BfrI BlnI BsiWI Bsp1407I BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DraI EagI EclXI Eco52I EcoRV EcoT22I FseI FspI HpaI KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### AflIII 743 ACACGT A^C[GA][CT]GT Alw44I 1686 GTGCAC G^TGCAC AocI 1845 CCTGAGG CC^T[ACGT]AGG ApaLI 1686 GTGCAC G^TGCAC BamHI 840 GGATCC G^GATCC BclI 1226 TGATCA T^GATCA BsiEI 1256 CGGTCG CG[GA][CT]^CG BsmBI 2573 GAGACG CGTCTC(1/5) BspDI 4057 ATCGAT AT^CGAT BsrDI 3710 CATTGC GCAATG(2/0) BstAPI 2389 GCAGTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1845 CCTGAGG CC^T[ACGT]AGG ClaI 4057 ATCGAT AT^CGAT DraIII 87 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1845 CCTGAGG CC^T[ACGT]AGG EcoNI 1955 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3880 GAATTC G^AATTC FbaI 1226 TGATCA T^GATCA NaeI 4582 GCCGGC GCC^GGC NgoMIV 4582 GCCGGC G^CCGGC NspI 2696 GCATGC [GA]CATG^[CT] PshAI 1188 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 1485 GAAGAGC GCTCTTC(1/4) SexAI 3212 ACCTGGT A^CC[AT]GGT SphI 2696 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AatII 2506 GACGTC GACGT^C 4200 GACGTC AccI 529 GTCTAC GT^[AC][GT]AC 1699 GTCTAC AccIII 3884 TCCGGA T^CCGGA 4415 TCCGGA AcsI 1520 AAATTC [GA]^AATT[CT] 3880 GAATTC Aor51HI 634 AGCGCT AGC^GCT 3515 AGCGCT ApoI 1520 AAATTC [GA]^AATT[CT] 3880 GAATTC Asp700I 3219 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4224 GAAGGACTTC AspHI 1686 GTGCAC G[AT]GC[AT]^C 3028 GAGCAC BcgI 3620 GCAACTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4064 GCATCAACATCG BglI 2033 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2068 GCCCTCTTGGC BglII 1031 AGATCT A^GATCT 2534 AGATCT BsaBI 4050 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4107 GATCACCATC BsaI 2827 GAGACC GGTCTC(1/5) 3469 GAGACC BseAI 3884 TCCGGA T^CCGGA 4415 TCCGGA BsiHKAI 1686 GTGCAC G[AT]GC[AT]^C 3028 GAGCAC BspEI 3884 TCCGGA T^CCGGA 4415 TCCGGA BspHI 977 TCATGA T^CATGA 2519 TCATGA BssSI 112 CTCGTG CACGAG(-5/-1) 1135 CTCGTG BstEII 3173 GGTCACC G^GT[ACGT]ACC 3717 GGTCACC BstPI 3173 GGTCACC G^GT[ACGT]ACC 3717 GGTCACC Eco47III 634 AGCGCT AGC^GCT 3515 AGCGCT EcoO65I 3173 GGTCACC G^GT[ACGT]ACC 3717 GGTCACC HincII 1270 GTCAAC GT[CT]^[GA]AC 3013 GTCAAC HindII 1270 GTCAAC GT[CT]^[GA]AC 3013 GTCAAC HindIII 1546 AAGCTT A^AGCTT 2875 AAGCTT MamI 4050 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4107 GATCACCATC MroI 3884 TCCGGA T^CCGGA 4415 TCCGGA NcoI 1799 CCATGG C^CATGG 3101 CCATGG PaeR7I 2570 CTCGAG C^TCGAG 3427 CTCGAG PpuMI 3911 AGGACCT [GA]G^G[AT]CC[CT] 4124 AGGACCC RcaI 977 TCATGA T^CATGA 2519 TCATGA ScaI 1148 AGTACT AGT^ACT 4178 AGTACT SmaI 2336 CCCGGG CCC^GGG 4322 CCCGGG XhoI 2570 CTCGAG C^TCGAG 3427 CTCGAG XmaCI 2336 CCCGGG C^CCGGG 4322 CCCGGG XmaI 2336 CCCGGG C^CCGGG 4322 CCCGGG XmnI 3219 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4224 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI ApaI AspEI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PflMI PleI PmaCI PmlI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII