Restriction map for
pF1KE9251
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BbeI BcgI BclI
BfrI BglI BglII BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MroI MseI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tru9I Tsp509I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 649 GTAGAC GT^[AC][GT]AC
AfaI 1626 GTAC GT^AC
AspI 1579 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1324 CCTAGG C^CTAGG
BalI 156 TGGCCA TGG^CCA
BamHI 1114 GGATCC G^GATCC
BbrPI 1594 CACGTG CAC^GTG
BciVI 60 GGATAC GTATCC(6/5)
BlnI 1324 CCTAGG C^CTAGG
BlpI 805 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 805 GCTGAGC GC^T[ACGT]AGC
BsaAI 1594 CACGTG [CT]AC^GT[GA]
BsiEI 819 CGGTCG CG[GA][CT]^CG
BsmI 534 GAATGC GAATGC(1/-1)
BspMI 1613 ACCTGC ACCTGC(4/8)
BsrBI 1606 GAGCGG CCGCTC(-3/-3)
CelII 805 GCTGAGC GC^T[ACGT]AGC
DraIII 1384 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1172 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1263 AGCGCC [GA]GCGC^[CT]
KspI 82 CCGCGG CCGC^GG
MluI 51 ACGCGT A^CGCGT
MluNI 156 TGGCCA TGG^CCA
MscI 156 TGGCCA TGG^CCA
PaeR7I 409 CTCGAG C^TCGAG
PflFI 1579 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1594 CACGTG CAC^GTG
PmlI 1594 CACGTG CAC^GTG
PvuII 1633 CAGCTG CAG^CTG
RsaI 1626 GTAC GT^AC
SacI 406 GAGCTC GAGCT^C
SacII 82 CCGCGG CCGC^GG
SspI 1466 AATATT AAT^ATT
StuI 610 AGGCCT AGG^CCT
TaqI 410 TCGA T^CGA
TfiI 295 GAATC G^A[AT]TC
Tth111I 1579 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 410 TCGA T^CGA
XhoI 409 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AflIII 51 ACGCGT A^C[GA][CT]GT
758 ACGTGT
AvaI 101 CTCGGG C^[CT]CG[GA]G
409 CTCGAG
BanI 658 GGTGCC G^G[CT][GA]CC
1610 GGCACC
BbsI 799 GAAGAC GAAGAC(2/6)
1669 GTCTTC
BbvCI 1081 GCTGAGG CCTCAGC(-5/-2)
1129 GCTGAGG
BpuAI 799 GAAGAC GAAGAC(2/6)
1669 GTCTTC
BseRI 695 CTCCTC GAGGAG(10/8)
901 GAGGAG
BsmFI 1086 GGGAC GGGAC(10/14)
1123 GGGAC
BsoBI 101 CTCGGG C^[CT]CG[GA]G
409 CTCGAG
BstXI 419 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1365 CCACAAGGCTGG
EaeI 156 TGGCCA [CT]^GGCC[GA]
1515 TGGCCG
HphI 1098 GGTGA GGTGA(8/7)
554 TCACC
MaeIII 1099 GTGAC ^GT[ACGT]AC
1573 GTCAC
PflMI 879 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1261 CCAGCGCCTGG
PpuMI 1059 AGGACCC [GA]G^G[AT]CC[CT]
1538 AGGACCT
Tsp45I 1099 GTGAC ^GT[GC]AC
1573 GTCAC
Van91I 879 CCAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1261 CCAGCGCCTGG
XcmI 230 CCAGTGGGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1412 CCAAGATGCCCATGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TseI TspRI XhoII