################################## # NO CUT ENZYMES ( 41 enzymes) # ################################## AclI AflII AscI AseI AsnI AvrII BciVI BfaI BfrI BlnI BspLU11I BsrDI Bst1107I BstBI BstZ17I CpoI DraI EcoT22I HpaI MaeI MfeI MluI MunI NdeI NheI NruI NsiI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SalI SfuI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 602 GGTACC G^GTACC AccIII 7298 TCCGGA T^CCGGA AflIII 4878 ACACGT A^C[GA][CT]GT Asp718I 602 GGTACC G^GTACC AspI 13081 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 12832 GGATCC G^GATCC BseAI 7298 TCCGGA T^CCGGA BsiWI 4039 CGTACG C^GTACG BsmI 4331 GCATTC GAATGC(1/-1) Bsp1407I 10751 TGTACA T^GTACA BspDI 12694 ATCGAT AT^CGAT BspEI 7298 TCCGGA T^CCGGA BspHI 9354 TCATGA T^CATGA BsrGI 10751 TGTACA T^GTACA ClaI 12694 ATCGAT AT^CGAT EcoRI 1780 GAATTC G^AATTC EcoRV 523 GATATC GAT^ATC HindIII 7560 AAGCTT A^AGCTT KpnI 602 GGTACC GGTAC^C MroI 7298 TCCGGA T^CCGGA MseI 1052 TTAA T^TAA NotI 5890 GCGGCCGC GC^GGCCGC PflFI 13081 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 10086 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 9354 TCATGA T^CATGA ScaI 9860 AGTACT AGT^ACT SexAI 12983 ACCTGGT A^CC[AT]GGT SphI 4268 GCATGC GCATG^C SspBI 10751 TGTACA T^GTACA TfiI 9939 GATTC G^A[AT]TC Tru9I 1052 TTAA T^TAA Tth111I 13081 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AatII 8788 GACGTC GACGT^C 12468 GACGTC AccI 8764 GTCTAC GT^[AC][GT]AC 11164 GTCTAC AcsI 1780 GAATTC [GA]^AATT[CT] 6595 AAATTC AgeI 11998 ACCGGT A^CCGGT 13057 ACCGGT AhdI 820 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 11584 GACCAGCTGTC ApoI 1780 GAATTC [GA]^AATT[CT] 6595 AAATTC Asp700I 216 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 13140 GAAGGCCTTC AspEI 820 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 11584 GACCAGCTGTC BclI 10934 TGATCA T^GATCA 12659 TGATCA Eam1105I 820 GACGAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 11584 GACCAGCTGTC FbaI 10934 TGATCA T^GATCA 12659 TGATCA NcoI 11759 CCATGG C^CATGG 12192 CCATGG NspI 4268 GCATGC [GA]CATG^[CT] 12782 ACATGC PinAI 11998 ACCGGT A^CCGGT 13057 ACCGGT Tsp509I 1781 AATT ^AATT 6596 AATT XmnI 216 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 13140 GAAGGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 169 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AspHI AvaI AvaII AviII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BcnI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PaeR7I PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SacII SapI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SfiI SfoI SgrAI SmaI SmlI Sse8387I StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI