Restriction map for
pF1KE3300
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# NO CUT ENZYMES ( 61 enzymes) #
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Acc65I AclI AflII AgeI AscI
AseI AsnI Asp718I AvrII BciVI
BclI BfaI BfrI BglII BlnI
BsiWI Bsp1407I BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DraI EcoO65I EcoRI
EcoRV FbaI FseI HindIII HpaI
KpnI MaeI MfeI MunI NheI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SapI SbfI SfuI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 24 enzymes) #
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AocI 5308 CCTGAGG CC^T[ACGT]AGG
Asp700I 4491 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1744 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2930 GGATCC G^GATCC
BbvCI 189 CCTCAGC CCTCAGC(-5/-2)
BsaBI 5934 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 5654 TCATGA T^CATGA
Bsu36I 5308 CCTGAGG CC^T[ACGT]AGG
DrdI 5617 GACGTCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 5308 CCTGAGG CC^T[ACGT]AGG
MamI 5934 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 4409 ACGCGT A^CGCGT
MseI 3716 TTAA T^TAA
NdeI 6274 CATATG CA^TATG
NotI 5221 GCGGCCGC GC^GGCCGC
PflFI 1744 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 775 GACGATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5654 TCATGA T^CATGA
SalI 2602 GTCGAC G^TCGAC
SfiI 7279 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TfiI 2272 GAATC G^A[AT]TC
Tru9I 3716 TTAA T^TAA
Tth111I 1744 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 4491 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccI 2602 GTCGAC GT^[AC][GT]AC
5026 GTCTAC
AccIII 5942 TCCGGA T^CCGGA
7650 TCCGGA
AcsI 4255 AAATTC [GA]^AATT[CT]
6882 GAATTT
AhdI 3688 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7552 GACCAGTGGTC
Aor51HI 3785 AGCGCT AGC^GCT
5112 AGCGCT
ApoI 4255 AAATTC [GA]^AATT[CT]
6882 GAATTT
AspEI 3688 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7552 GACCAGTGGTC
BbsI 4813 GTCTTC GAAGAC(2/6)
5926 GTCTTC
BlpI 4785 GCTCAGC GC^T[ACGT]AGC
7265 GCTCAGC
Bpu1102I 4785 GCTCAGC GC^T[ACGT]AGC
7265 GCTCAGC
BpuAI 4813 GTCTTC GAAGAC(2/6)
5926 GTCTTC
BseAI 5942 TCCGGA T^CCGGA
7650 TCCGGA
BspEI 5942 TCCGGA T^CCGGA
7650 TCCGGA
BssSI 56 CACGAG CACGAG(-5/-1)
721 CACGAG
BstYI 2930 GGATCC [GA]^GATC[CT]
5933 AGATCC
CelII 4785 GCTCAGC GC^T[ACGT]AGC
7265 GCTCAGC
Eam1105I 3688 GACCCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
7552 GACCAGTGGTC
EarI 4543 CTCTTC CTCTTC(1/4)
5704 GAAGAG
Eco47III 3785 AGCGCT AGC^GCT
5112 AGCGCT
Ksp632I 4543 CTCTTC CTCTTC(1/4)
5704 GAAGAG
MflI 2930 GGATCC [GA]^GATC[CT]
5933 AGATCC
MroI 5942 TCCGGA T^CCGGA
7650 TCCGGA
SgrAI 5189 CGCCGGCG C[GA]^CCGG[CT]G
5278 CGCCGGCG
SmaI 556 CCCGGG CCC^GGG
6206 CCCGGG
Tsp509I 4256 AATT ^AATT
6883 AATT
XhoII 2930 GGATCC [GA]^GATC[CT]
5933 AGATCC
XmaCI 556 CCCGGG C^CCGGG
6206 CCCGGG
XmaI 556 CCCGGG C^CCGGG
6206 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaI ApaLI AspHI AvaI AvaII
AviII BalI BanI BanII BbeI
BbrPI BbvI BcgI BcnI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspDI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I ClaI
DdeI DpnII DraII DraIII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI KspI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NsiI
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SfoI SmlI
SphI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI