Restriction map for
pF1KE5297
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# NO CUT ENZYMES ( 63 enzymes) #
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AatII AccIII AflII AgeI AhdI
AscI AspEI AviII AvrII BfrI
BlnI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI EagI
Eam1105I EarI EclXI Eco52I EcoRV
EcoT22I FseI FspI HpaI Ksp632I
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PvuI
RcaI SacI SalI SapI SbfI
ScaI SfiI SfuI SnaBI SpeI
Sse8387I SwaI XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 2402 GGTACC G^GTACC
AccI 670 GTCTAC GT^[AC][GT]AC
AclI 726 AACGTT AA^CGTT
AocI 2356 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2223 AGCGCT AGC^GCT
ApaI 3100 GGGCCC GGGCC^C
AseI 979 ATTAAT AT^TAAT
AsnI 979 ATTAAT AT^TAAT
Asp718I 2402 GGTACC G^GTACC
AspI 1000 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1034 GGATCC G^GATCC
BcgI 2306 GCAGCTTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1657 GGATAC GTATCC(6/5)
BglI 442 GCCGAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1364 AGATCT A^GATCT
BsmBI 2719 GAGACG CGTCTC(1/5)
BsrBI 894 GAGCGG CCGCTC(-3/-3)
BsrDI 2541 CATTGC GCAATG(2/0)
BstAPI 2013 GCATGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2517 GGTGACC G^GT[ACGT]ACC
BstPI 2517 GGTGACC G^GT[ACGT]ACC
BstXI 944 CCACCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2356 CCTGAGG CC^T[ACGT]AGG
CpoI 1049 CGGACCG CG^G[AT]CCG
DraIII 2257 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2223 AGCGCT AGC^GCT
Eco81I 2356 CCTGAGG CC^T[ACGT]AGG
EcoNI 1038 CCTCGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2517 GGTGACC G^GT[ACGT]ACC
HincII 729 GTTGAC GT[CT]^[GA]AC
HindII 729 GTTGAC GT[CT]^[GA]AC
HindIII 1642 AAGCTT A^AGCTT
KpnI 2402 GGTACC GGTAC^C
PaeR7I 2829 CTCGAG C^TCGAG
PflFI 1000 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3213 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 979 ATTAAT AT^TAAT
Psp1406I 726 AACGTT AA^CGTT
RsrII 1049 CGGACCG CG^G[AT]CCG
SexAI 2893 ACCAGGT A^CC[AT]GGT
SgrAI 3271 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2829 CTCGAG C^T[CT][GA]AG
SphI 1336 GCATGC GCATG^C
SspI 740 AATATT AAT^ATT
Tth111I 1000 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2829 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 35 enzymes) #
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Alw44I 1651 GTGCAC G^TGCAC
2634 GTGCAC
ApaLI 1651 GTGCAC G^TGCAC
2634 GTGCAC
Asp700I 969 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1152 GAAGCAGTTC
BalI 1258 TGGCCA TGG^CCA
1529 TGGCCA
BbrPI 1411 CACGTG CAC^GTG
2257 CACGTG
BclI 509 TGATCA T^GATCA
1820 TGATCA
BfaI 175 CTAG C^TAG
2104 CTAG
BsaAI 1411 CACGTG [CT]AC^GT[GA]
2257 CACGTG
BsaI 719 GAGACC GGTCTC(1/5)
2331 GGTCTC
BsaWI 2087 ACCGGA [AT]^CCGG[AT]
3248 ACCGGA
Bsp1407I 638 TGTACA T^GTACA
1718 TGTACA
BspLU11I 674 ACATGT A^CATGT
2237 ACATGT
BspMI 590 ACCTGC ACCTGC(4/8)
1406 ACCTGC
BsrGI 638 TGTACA T^GTACA
1718 TGTACA
DraI 660 TTTAAA TTT^AAA
1027 TTTAAA
DrdI 1264 GACTGTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2665 GACTGCTTCGTC
EcoRI 466 GAATTC G^AATTC
2575 GAATTC
FbaI 509 TGATCA T^GATCA
1820 TGATCA
HgaI 2185 GACGC GACGC(5/10)
2558 GCGTC
KspI 834 CCGCGG CCGC^GG
2552 CCGCGG
MaeI 175 CTAG C^TAG
2104 CTAG
MluNI 1258 TGGCCA TGG^CCA
1529 TGGCCA
MscI 1258 TGGCCA TGG^CCA
1529 TGGCCA
NaeI 3063 GCCGGC GCC^GGC
3272 GCCGGC
NgoMIV 3063 GCCGGC G^CCGGC
3272 GCCGGC
PflMI 423 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2853 CCAGCCCCTGG
PmaCI 1411 CACGTG CAC^GTG
2257 CACGTG
PmlI 1411 CACGTG CAC^GTG
2257 CACGTG
SacII 834 CCGCGG CCGC^GG
2552 CCGCGG
SmaI 1958 CCCGGG CCC^GGG
2285 CCCGGG
SspBI 638 TGTACA T^GTACA
1718 TGTACA
Van91I 423 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2853 CCAGCCCCTGG
XmaCI 1958 CCCGGG C^CCGGG
2285 CCCGGG
XmaI 1958 CCCGGG C^CCGGG
2285 CCCGGG
XmnI 969 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1152 GAAGCAGTTC
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# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII