################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AclI AflII AgeI AhdI AscI AseI AsnI AspEI AviII AvrII BbeI BciVI BfrI BglII BlnI BsiWI BsmBI Bsp1407I BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DraI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FseI FspI HpaI KasI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SapI ScaI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 5359 GACGTC GACGT^C Acc65I 2309 GGTACC G^GTACC AccII 3861 CGCG CG^CG AflIII 3932 ACATGT A^C[GA][CT]GT Alw44I 1735 GTGCAC G^TGCAC Aor51HI 4088 AGCGCT AGC^GCT ApaLI 1735 GTGCAC G^TGCAC Asp718I 2309 GGTACC G^GTACC BamHI 503 GGATCC G^GATCC BbrPI 3502 CACGTG CAC^GTG BclI 809 TGATCA T^GATCA BsaAI 3502 CACGTG [CT]AC^GT[GA] BsaBI 2697 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 4829 CGACCG CG[GA][CT]^CG BspDI 2389 ATCGAT AT^CGAT BspHI 383 TCATGA T^CATGA BspLU11I 3932 ACATGT A^CATGT BsrBI 1535 CCGCTC CCGCTC(-3/-3) BsrDI 4473 CATTGC GCAATG(2/0) BsrFI 5561 ACCGGC [GA]^CCGG[CT] BssSI 2314 CACGAG CACGAG(-5/-1) BstUI 3861 CGCG CG^CG Cfr10I 5561 ACCGGC [GA]^CCGG[CT] ClaI 2389 ATCGAT AT^CGAT DraIII 4926 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1587 GACAGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4088 AGCGCT AGC^GCT EcoRI 5161 GAATTC G^AATTC FbaI 809 TGATCA T^GATCA HaeII 4088 AGCGCT [GA]GCGC^[CT] HincII 4827 GTCGAC GT[CT]^[GA]AC HindII 4827 GTCGAC GT[CT]^[GA]AC HindIII 3755 AAGCTT A^AGCTT KpnI 2309 GGTACC GGTAC^C KspI 3860 CCGCGG CCGC^GG MamI 2697 GATTCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 3861 CGCG CG^CG PaeR7I 163 CTCGAG C^TCGAG PmaCI 3502 CACGTG CAC^GTG PmlI 3502 CACGTG CAC^GTG RcaI 383 TCATGA T^CATGA SacII 3860 CCGCGG CCGC^GG SalI 4827 GTCGAC G^TCGAC SbfI 1909 CCTGCAGG CCTGCA^GG SphI 519 GCATGC GCATG^C Sse8387I 1909 CCTGCAGG CCTGCA^GG XhoI 163 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 1295 GTAGAC GT^[AC][GT]AC 4827 GTCGAC AccIII 651 TCCGGA T^CCGGA 4619 TCCGGA AcsI 768 AAATTC [GA]^AATT[CT] 5161 GAATTC AcyI 3069 GGCGTC G[GA]^CG[CT]C 5359 GACGTC AocI 3724 CCTGAGG CC^T[ACGT]AGG 4821 CCTCAGG ApoI 768 AAATTC [GA]^AATT[CT] 5161 GAATTC Asp700I 117 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2769 GAATCTCTTC AspI 369 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5488 GACCAGGTC BcgI 4467 CGACATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2259 GCAGCCACCTCG BsaHI 3069 GGCGTC G[GA]^CG[CT]C 5359 GACGTC BseAI 651 TCCGGA T^CCGGA 4619 TCCGGA BsmI 1122 GCATTC GAATGC(1/-1) 4942 GCATTC BspEI 651 TCCGGA T^CCGGA 4619 TCCGGA Bsu36I 3724 CCTGAGG CC^T[ACGT]AGG 4821 CCTCAGG Eco81I 3724 CCTGAGG CC^T[ACGT]AGG 4821 CCTCAGG Hin1I 3069 GGCGTC G[GA]^CG[CT]C 5359 GACGTC MroI 651 TCCGGA T^CCGGA 4619 TCCGGA PflFI 369 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5488 GACCAGGTC Tth111I 369 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 5488 GACCAGGTC XmnI 117 GAACGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2769 GAATCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI