Restriction map for
pF1KE2491
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# NO CUT ENZYMES ( 47 enzymes) #
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Acc65I AclI AflII AseI AsnI
Asp718I BfrI BglII BsiWI BsmI
BspDI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EcoT22I HindIII HpaI
KpnI MfeI MluI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PmeI PshBI Psp1406I
RsrII SbfI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XbaI XhoI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AccIII 2558 TCCGGA T^CCGGA
AcsI 2185 GAATTC [GA]^AATT[CT]
AgeI 3842 ACCGGT A^CCGGT
ApoI 2185 GAATTC [GA]^AATT[CT]
AvrII 6091 CCTAGG C^CTAGG
BcgI 6600 CGAGGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 672 GGATAC GTATCC(6/5)
BlnI 6091 CCTAGG C^CTAGG
BseAI 2558 TCCGGA T^CCGGA
Bsp1407I 2939 TGTACA T^GTACA
BspEI 2558 TCCGGA T^CCGGA
BsrDI 5163 CATTGC GCAATG(2/0)
BsrGI 2939 TGTACA T^GTACA
EcoRI 2185 GAATTC G^AATTC
EcoRV 4269 GATATC GAT^ATC
FseI 3614 GGCCGGCC GGCCGG^CC
MroI 2558 TCCGGA T^CCGGA
NotI 261 GCGGCCGC GC^GGCCGC
PinAI 3842 ACCGGT A^CCGGT
PshAI 3099 GACGTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3645 CGATCG CGAT^CG
SalI 4555 GTCGAC G^TCGAC
SspBI 2939 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AatII 2197 GACGTC GACGT^C
3099 GACGTC
AhdI 1702 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3094 GACAAGACGTC
Aor51HI 392 AGCGCT AGC^GCT
6746 AGCGCT
AscI 46 GGCGCGCC GG^CGCGCC
1660 GGCGCGCC
AspEI 1702 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3094 GACAAGACGTC
BamHI 650 GGATCC G^GATCC
6105 GGATCC
BspLU11I 4643 ACATGT A^CATGT
5795 ACATGT
BssSI 2713 CTCGTG CACGAG(-5/-1)
3040 CTCGTG
DrdI 6292 GACGAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6814 GACCTCGGGGTC
Eam1105I 1702 GACGCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3094 GACAAGACGTC
Eco47III 392 AGCGCT AGC^GCT
6746 AGCGCT
PflMI 2709 CCAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7033 CCAGGGGGTGG
SexAI 1547 ACCTGGT A^CC[AT]GGT
5738 ACCTGGT
SfiI 219 GGCCTTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3605 GGCCCCTGCGGCC
Tsp509I 2186 AATT ^AATT
3994 AATT
Van91I 2709 CCAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7033 CCAGGGGGTGG
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# 3 OR MORE CUT ENZYMES ( 172 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp700I AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI SacII SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SfoI
SmaI SmlI SphI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
Tth111I TthHB8I XcmI XhoII XmaCI
XmaI XmnI