Restriction map for
pF1KE2548
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# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvrII BbeI BclI BfrI
BlnI BsaBI BsaWI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI Eam1105I Eco47III EcoO65I
EcoRV EcoT22I FbaI FseI HaeII
HincII HindII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PflMI PinAI PmeI
PshAI PshBI PvuI RcaI RsrII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI StuI SwaI Van91I XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1244 GACGTC GACGT^C
AclI 584 AACGTT AA^CGTT
AcsI 2446 GAATTC [GA]^AATT[CT]
AcyI 1244 GACGTC G[GA]^CG[CT]C
AflIII 2063 ACACGT A^C[GA][CT]GT
AocI 294 CCTCAGG CC^T[ACGT]AGG
ApoI 2446 GAATTC [GA]^AATT[CT]
AviII 47 TGCGCA TGC^GCA
BamHI 1023 GGATCC G^GATCC
BbrPI 2064 CACGTG CAC^GTG
BbvCI 276 CCTCAGC CCTCAGC(-5/-2)
BcgI 1983 CGAGACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2192 GCCTGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 681 AGATCT A^GATCT
BlpI 2158 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2158 GCTGAGC GC^T[ACGT]AGC
BsaAI 2064 CACGTG [CT]AC^GT[GA]
BsaHI 1244 GACGTC G[GA]^CG[CT]C
BsgI 1539 GTGCAG GTGCAG(16/14)
BsiEI 17 CGGCCG CG[GA][CT]^CG
BsmI 2052 GCATTC GAATGC(1/-1)
BsrDI 365 GCAATG GCAATG(2/0)
BsrFI 19 GCCGGC [GA]^CCGG[CT]
BstAPI 2043 GCACACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2690 CCAGGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 294 CCTCAGG CC^T[ACGT]AGG
CelII 2158 GCTGAGC GC^T[ACGT]AGC
Cfr10I 19 GCCGGC [GA]^CCGG[CT]
DrdI 1723 GACGGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 17 CGGCCG C^GGCCG
EclXI 17 CGGCCG C^GGCCG
Eco52I 17 CGGCCG C^GGCCG
Eco81I 294 CCTCAGG CC^T[ACGT]AGG
EcoNI 315 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2446 GAATTC G^AATTC
FspI 47 TGCGCA TGC^GCA
Hin1I 1244 GACGTC G[GA]^CG[CT]C
HindIII 1485 AAGCTT A^AGCTT
KspI 31 CCGCGG CCGC^GG
MslI 2623 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 19 GCCGGC GCC^GGC
NcoI 2397 CCATGG C^CATGG
NgoMIV 19 GCCGGC G^CCGGC
NheI 639 GCTAGC G^CTAGC
NspI 687 GCATGC [GA]CATG^[CT]
PleI 2642 GACTC GAGTC(4/5)
PmaCI 2064 CACGTG CAC^GTG
PmlI 2064 CACGTG CAC^GTG
Psp1406I 584 AACGTT AA^CGTT
SacI 1336 GAGCTC GAGCT^C
SacII 31 CCGCGG CCGC^GG
SbfI 1127 CCTGCAGG CCTGCA^GG
SexAI 767 ACCTGGT A^CC[AT]GGT
SphI 687 GCATGC GCATG^C
Sse8387I 1127 CCTGCAGG CCTGCA^GG
XbaI 1059 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1633 GTAGAC GT^[AC][GT]AC
2023 GTCTAC
Alw44I 1642 GTGCAC G^TGCAC
2041 GTGCAC
AlwNI 1660 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2740 CAGAAGCTG
ApaLI 1642 GTGCAC G^TGCAC
2041 GTGCAC
Asp700I 582 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
774 GAAGTGGTTC
AspI 1756 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACTGTGTC
BalI 224 TGGCCA TGG^CCA
2552 TGGCCA
BanI 1123 GGCACC G^G[CT][GA]CC
2250 GGTGCC
BciVI 2428 GGATAC GTATCC(6/5)
2693 GGATAC
BsaI 1813 GAGACC GGTCTC(1/5)
1821 GAGACC
BsrBI 1412 CCGCTC CCGCTC(-3/-3)
1261 GAGCGG
BstYI 681 AGATCT [GA]^GATC[CT]
1023 GGATCC
CfoI 14 GCGC GCG^C
48 GCGC
DraIII 1645 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2338 CACCGCGTG
HgaI 38 GACGC GACGC(5/10)
2586 GCGTC
HhaI 14 GCGC GCG^C
48 GCGC
HinP1I 14 GCGC G^CGC
48 GCGC
MflI 681 AGATCT [GA]^GATC[CT]
1023 GGATCC
MluNI 224 TGGCCA TGG^CCA
2552 TGGCCA
MscI 224 TGGCCA TGG^CCA
2552 TGGCCA
MseI 239 TTAA T^TAA
800 TTAA
PaeR7I 737 CTCGAG C^TCGAG
875 CTCGAG
PflFI 1756 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACTGTGTC
PpuMI 1453 AGGACCC [GA]G^G[AT]CC[CT]
1478 AGGACCC
SapI 132 GAAGAGC GCTCTTC(1/4)
1508 GCTCTTC
TfiI 912 GATTC G^A[AT]TC
1375 GATTC
Tru9I 239 TTAA T^TAA
800 TTAA
Tth111I 1756 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2365 GACTGTGTC
XcmI 1694 CCATGACCTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2373 CCAGGCCATCACTGG
XhoI 737 CTCGAG C^TCGAG
875 CTCGAG
XhoII 681 AGATCT [GA]^GATC[CT]
1023 GGATCC
XmnI 582 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
774 GAAGTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I