################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AclI AflIII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AviII BglI BlpI Bpu1102I BsaBI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI CelII Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HincII HindII HpaI KpnI KspI MamI MluI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 714 GTATAC GT^[AC][GT]AC AccIII 3153 TCCGGA T^CCGGA AflII 1795 CTTAAG C^TTAAG AlwNI 2762 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2430 CCTGAGG CC^T[ACGT]AGG Asp700I 3219 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 30 GAGCTC G[AT]GC[AT]^C AspI 3055 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2551 GGATCC G^GATCC BbrPI 1504 CACGTG CAC^GTG BbvCI 2843 CCTCAGC CCTCAGC(-5/-2) BciVI 1846 GGATAC GTATCC(6/5) BfrI 1795 CTTAAG C^TTAAG BglII 1365 AGATCT A^GATCT BsaAI 1504 CACGTG [CT]AC^GT[GA] BsaWI 3153 TCCGGA [AT]^CCGG[AT] BseAI 3153 TCCGGA T^CCGGA BsgI 71 CTGCAC GTGCAG(16/14) BsiHKAI 30 GAGCTC G[AT]GC[AT]^C BsmI 2612 GAATGC GAATGC(1/-1) BspEI 3153 TCCGGA T^CCGGA Bst1107I 714 GTATAC GTA^TAC BstZ17I 714 GTATAC GTA^TAC Bsu36I 2430 CCTGAGG CC^T[ACGT]AGG DraI 2445 TTTAAA TTT^AAA DraIII 1340 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2430 CCTGAGG CC^T[ACGT]AGG EcoRI 2171 GAATTC G^AATTC EcoT22I 2855 ATGCAT ATGCA^T HgaI 52 GCGTC GACGC(5/10) HindIII 1136 AAGCTT A^AGCTT MfeI 731 CAATTG C^AATTG MroI 3153 TCCGGA T^CCGGA MunI 731 CAATTG C^AATTG NdeI 2114 CATATG CA^TATG NsiI 2855 ATGCAT ATGCA^T NspI 1261 GCATGT [GA]CATG^[CT] PflFI 3055 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1504 CACGTG CAC^GTG PmlI 1504 CACGTG CAC^GTG PshAI 358 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 30 GAGCTC GAGCT^C SapI 1003 GAAGAGC GCTCTTC(1/4) SexAI 500 ACCAGGT A^CC[AT]GGT StuI 1461 AGGCCT AGG^CCT Tth111I 3055 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3219 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 24 CGCG CG^CG 122 CGCG AcyI 39 GGCGCC G[GA]^CG[CT]C 131 GGCGCC AhdI 1492 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3320 GACGAATAGTC AspEI 1492 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3320 GACGAATAGTC AvrII 271 CCTAGG C^CTAGG 1285 CCTAGG BanI 39 GGCGCC G^G[CT][GA]CC 131 GGCGCC BanII 30 GAGCTC G[GA]GC[CT]^C 1335 GAGCCC BbeI 39 GGCGCC GGCGC^C 131 GGCGCC BcgI 63 CGAAGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 726 CGACACAATTGC BclI 1466 TGATCA T^GATCA 1991 TGATCA BlnI 271 CCTAGG C^CTAGG 1285 CCTAGG BpmI 17 CTCCAG CTGGAG(16/14) 2739 CTCCAG BsaHI 39 GGCGCC G[GA]^CG[CT]C 131 GGCGCC BsmBI 369 CGTCTC CGTCTC(1/5) 3318 GAGACG BssSI 99 CTCGTG CACGAG(-5/-1) 1927 CTCGTG BstAPI 2466 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2848 GCAAGCGATGC BstUI 24 CGCG CG^CG 122 CGCG BstXI 2399 CCATTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2741 CCAGAGTGCTGG Eam1105I 1492 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3320 GACGAATAGTC EcoNI 119 CCTCGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3108 CCTGGTGGAGG FauI 2463 CCCGC CCCGC(4/6) 151 GCGGG FbaI 1466 TGATCA T^GATCA 1991 TGATCA HaeII 39 GGCGCC [GA]GCGC^[CT] 131 GGCGCC Hin1I 39 GGCGCC G[GA]^CG[CT]C 131 GGCGCC KasI 39 GGCGCC G^GCGCC 131 GGCGCC MvnI 24 CGCG CG^CG 122 CGCG NarI 39 GGCGCC GG^CGCC 131 GGCGCC PflMI 439 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2798 CCATAAGGTGG PpuMI 115 GGGACCT [GA]G^G[AT]CC[CT] 200 AGGACCC PstI 360 CTGCAG CTGCA^G 1074 CTGCAG ScaI 1485 AGTACT AGT^ACT 1718 AGTACT SfoI 39 GGCGCC GGC^GCC 131 GGCGCC SmlI 1795 CTTAAG C^T[CT][GA]AG 2166 CTCAAG TaqI 725 TCGA T^CGA 2670 TCGA TthHB8I 725 TCGA T^CGA 2670 TCGA Van91I 439 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2798 CCATAAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaI AvaII BalI BbsI BbvI BcnI BfaI BmyI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII XmaCI XmaI