Restriction map for
pF1KE3542
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AviII BglI
BlpI Bpu1102I BsaBI BsiEI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HincII
HindII HpaI KpnI KspI MamI
MluI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 714 GTATAC GT^[AC][GT]AC
AccIII 3153 TCCGGA T^CCGGA
AflII 1795 CTTAAG C^TTAAG
AlwNI 2762 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2430 CCTGAGG CC^T[ACGT]AGG
Asp700I 3219 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 30 GAGCTC G[AT]GC[AT]^C
AspI 3055 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2551 GGATCC G^GATCC
BbrPI 1504 CACGTG CAC^GTG
BbvCI 2843 CCTCAGC CCTCAGC(-5/-2)
BciVI 1846 GGATAC GTATCC(6/5)
BfrI 1795 CTTAAG C^TTAAG
BglII 1365 AGATCT A^GATCT
BsaAI 1504 CACGTG [CT]AC^GT[GA]
BsaWI 3153 TCCGGA [AT]^CCGG[AT]
BseAI 3153 TCCGGA T^CCGGA
BsgI 71 CTGCAC GTGCAG(16/14)
BsiHKAI 30 GAGCTC G[AT]GC[AT]^C
BsmI 2612 GAATGC GAATGC(1/-1)
BspEI 3153 TCCGGA T^CCGGA
Bst1107I 714 GTATAC GTA^TAC
BstZ17I 714 GTATAC GTA^TAC
Bsu36I 2430 CCTGAGG CC^T[ACGT]AGG
DraI 2445 TTTAAA TTT^AAA
DraIII 1340 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2430 CCTGAGG CC^T[ACGT]AGG
EcoRI 2171 GAATTC G^AATTC
EcoT22I 2855 ATGCAT ATGCA^T
HgaI 52 GCGTC GACGC(5/10)
HindIII 1136 AAGCTT A^AGCTT
MfeI 731 CAATTG C^AATTG
MroI 3153 TCCGGA T^CCGGA
MunI 731 CAATTG C^AATTG
NdeI 2114 CATATG CA^TATG
NsiI 2855 ATGCAT ATGCA^T
NspI 1261 GCATGT [GA]CATG^[CT]
PflFI 3055 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1504 CACGTG CAC^GTG
PmlI 1504 CACGTG CAC^GTG
PshAI 358 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 30 GAGCTC GAGCT^C
SapI 1003 GAAGAGC GCTCTTC(1/4)
SexAI 500 ACCAGGT A^CC[AT]GGT
StuI 1461 AGGCCT AGG^CCT
Tth111I 3055 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3219 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccII 24 CGCG CG^CG
122 CGCG
AcyI 39 GGCGCC G[GA]^CG[CT]C
131 GGCGCC
AhdI 1492 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3320 GACGAATAGTC
AspEI 1492 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3320 GACGAATAGTC
AvrII 271 CCTAGG C^CTAGG
1285 CCTAGG
BanI 39 GGCGCC G^G[CT][GA]CC
131 GGCGCC
BanII 30 GAGCTC G[GA]GC[CT]^C
1335 GAGCCC
BbeI 39 GGCGCC GGCGC^C
131 GGCGCC
BcgI 63 CGAAGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
726 CGACACAATTGC
BclI 1466 TGATCA T^GATCA
1991 TGATCA
BlnI 271 CCTAGG C^CTAGG
1285 CCTAGG
BpmI 17 CTCCAG CTGGAG(16/14)
2739 CTCCAG
BsaHI 39 GGCGCC G[GA]^CG[CT]C
131 GGCGCC
BsmBI 369 CGTCTC CGTCTC(1/5)
3318 GAGACG
BssSI 99 CTCGTG CACGAG(-5/-1)
1927 CTCGTG
BstAPI 2466 GCATCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2848 GCAAGCGATGC
BstUI 24 CGCG CG^CG
122 CGCG
BstXI 2399 CCATTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2741 CCAGAGTGCTGG
Eam1105I 1492 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3320 GACGAATAGTC
EcoNI 119 CCTCGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3108 CCTGGTGGAGG
FauI 2463 CCCGC CCCGC(4/6)
151 GCGGG
FbaI 1466 TGATCA T^GATCA
1991 TGATCA
HaeII 39 GGCGCC [GA]GCGC^[CT]
131 GGCGCC
Hin1I 39 GGCGCC G[GA]^CG[CT]C
131 GGCGCC
KasI 39 GGCGCC G^GCGCC
131 GGCGCC
MvnI 24 CGCG CG^CG
122 CGCG
NarI 39 GGCGCC GG^CGCC
131 GGCGCC
PflMI 439 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2798 CCATAAGGTGG
PpuMI 115 GGGACCT [GA]G^G[AT]CC[CT]
200 AGGACCC
PstI 360 CTGCAG CTGCA^G
1074 CTGCAG
ScaI 1485 AGTACT AGT^ACT
1718 AGTACT
SfoI 39 GGCGCC GGC^GCC
131 GGCGCC
SmlI 1795 CTTAAG C^T[CT][GA]AG
2166 CTCAAG
TaqI 725 TCGA T^CGA
2670 TCGA
TthHB8I 725 TCGA T^CGA
2670 TCGA
Van91I 439 CCATTAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2798 CCATAAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaI AvaII BalI BbsI
BbvI BcnI BfaI BmyI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII XmaCI XmaI