Restriction map for
pF1KB8518
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BbeI BbrPI BcgI BclI
BfrI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstUI BstZ17I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV FbaI FseI HaeII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 1398 GACGTC GACGT^C
AcyI 1398 GACGTC G[GA]^CG[CT]C
AocI 1030 CCTGAGG CC^T[ACGT]AGG
ApaI 2077 GGGCCC GGGCC^C
AvaI 586 CTCGGG C^[CT]CG[GA]G
AviII 1216 TGCGCA TGC^GCA
BamHI 638 GGATCC G^GATCC
BanI 1779 GGCACC G^G[CT][GA]CC
BbvCI 1108 GCTGAGG CCTCAGC(-5/-2)
BciVI 1707 GTATCC GTATCC(6/5)
BglII 2381 AGATCT A^GATCT
BsaAI 2440 TACGTG [CT]AC^GT[GA]
BsaHI 1398 GACGTC G[GA]^CG[CT]C
BsaI 2064 GGTCTC GGTCTC(1/5)
BsoBI 586 CTCGGG C^[CT]CG[GA]G
BspDI 563 ATCGAT AT^CGAT
BspMI 2121 GCAGGT ACCTGC(4/8)
BsrBI 1672 CCGCTC CCGCTC(-3/-3)
BsrDI 2112 CATTGC GCAATG(2/0)
BsrFI 1097 GCCGGC [GA]^CCGG[CT]
BstEII 1981 GGTGACC G^GT[ACGT]ACC
BstPI 1981 GGTGACC G^GT[ACGT]ACC
BstXI 2117 CCAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1030 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1097 GCCGGC [GA]^CCGG[CT]
ClaI 563 ATCGAT AT^CGAT
Eco81I 1030 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1981 GGTGACC G^GT[ACGT]ACC
EcoRI 2561 GAATTC G^AATTC
FauI 645 GCGGG CCCGC(4/6)
FspI 1216 TGCGCA TGC^GCA
Hin1I 1398 GACGTC G[GA]^CG[CT]C
HincII 13 GTCAAC GT[CT]^[GA]AC
HindII 13 GTCAAC GT[CT]^[GA]AC
HindIII 1306 AAGCTT A^AGCTT
NaeI 1097 GCCGGC GCC^GGC
NgoMIV 1097 GCCGGC G^CCGGC
PflMI 725 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1325 GGGACCC [GA]G^G[AT]CC[CT]
SapI 1209 GCTCTTC GCTCTTC(1/4)
SphI 1316 GCATGC GCATG^C
SspI 1515 AATATT AAT^ATT
TaqI 564 TCGA T^CGA
TthHB8I 564 TCGA T^CGA
Van91I 725 CCATCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1356 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 28 enzymes) #
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BbsI 268 GAAGAC GAAGAC(2/6)
1245 GAAGAC
BcnI 218 CCCGG CC^[GC]GG
231 CCGGG
BglI 1882 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2192 GCCTGGTGGGC
BlpI 1695 GCTCAGC GC^T[ACGT]AGC
2098 GCTGAGC
Bpu1102I 1695 GCTCAGC GC^T[ACGT]AGC
2098 GCTGAGC
BpuAI 268 GAAGAC GAAGAC(2/6)
1245 GAAGAC
BsmI 1297 GAATGC GAATGC(1/-1)
710 GCATTC
Bsp1407I 697 TGTACA T^GTACA
2492 TGTACA
BsrGI 697 TGTACA T^GTACA
2492 TGTACA
BstAPI 284 GCATTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
988 GCAAAAGATGC
CelII 1695 GCTCAGC GC^T[ACGT]AGC
2098 GCTGAGC
CfoI 950 GCGC GCG^C
1217 GCGC
DraII 1325 GGGACCC [GA]G^G[ACGT]CC[CT]
2077 GGGCCCC
DraIII 2017 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
2019 CACCGTGTG
DsaI 1331 CCACGG C^C[GA][CT]GG
2483 CCGTGG
EaeI 299 CGGCCA [CT]^GGCC[GA]
1099 CGGCCA
EcoO109I 1325 GGGACCC [GA]G^G[ACGT]CC[CT]
2077 GGGCCCC
EcoT14I 884 CCAAGG C^C[AT][AT]GG
1711 CCAAGG
HgaI 2284 GACGC GACGC(5/10)
2505 GCGTC
HhaI 950 GCGC GCG^C
1217 GCGC
HinP1I 950 GCGC G^CGC
1217 GCGC
MseI 203 TTAA T^TAA
406 TTAA
NciI 218 CCCGG CC^[GC]GG
231 CCGGG
ScaI 877 AGTACT AGT^ACT
1187 AGTACT
SmlI 522 CTTGAG C^T[CT][GA]AG
801 CTCAAG
SspBI 697 TGTACA T^GTACA
2492 TGTACA
StyI 884 CCAAGG C^C[AT][AT]GG
1711 CCAAGG
Tru9I 203 TTAA T^TAA
406 TTAA
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# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EarI EcoRII EcoT22I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI TseI Tsp45I Tsp509I
TspRI XcmI XhoII