Restriction map for
pF1KE3386
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AgeI Aor51HI AscI Asp718I
AspI AvaI AviII AvrII BanI
BbeI BbvCI BcgI BcnI BfrI
BlnI BseAI BsiEI BsiWI BsoBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I ClaI CpoI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoRV
FauI FseI FspI HaeII HpaI
KasI KpnI Ksp632I KspI MluI
MroI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 1760 AACGTT AA^CGTT
AcyI 2825 GACGCC G[GA]^CG[CT]C
Alw44I 2758 GTGCAC G^TGCAC
AocI 2877 CCTCAGG CC^T[ACGT]AGG
ApaLI 2758 GTGCAC G^TGCAC
AseI 1855 ATTAAT AT^TAAT
AsnI 1855 ATTAAT AT^TAAT
BamHI 1126 GGATCC G^GATCC
BbrPI 1140 CACGTG CAC^GTG
BclI 230 TGATCA T^GATCA
BglI 3068 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 555 AGATCT A^GATCT
BlpI 1518 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1518 GCTCAGC GC^T[ACGT]AGC
BsaAI 1140 CACGTG [CT]AC^GT[GA]
BsaBI 2350 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2825 GACGCC G[GA]^CG[CT]C
BsaWI 1049 ACCGGA [AT]^CCGG[AT]
BsmBI 2158 CGTCTC CGTCTC(1/5)
BspHI 1554 TCATGA T^CATGA
BsrFI 2690 GCCGGT [GA]^CCGG[CT]
BssSI 1799 CACGAG CACGAG(-5/-1)
Bsu36I 2877 CCTCAGG CC^T[ACGT]AGG
CelII 1518 GCTCAGC GC^T[ACGT]AGC
CfoI 3066 GCGC GCG^C
Cfr10I 2690 GCCGGT [GA]^CCGG[CT]
DrdI 2424 GACTAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2877 CCTCAGG CC^T[ACGT]AGG
EcoRI 111 GAATTC G^AATTC
FbaI 230 TGATCA T^GATCA
HgaI 2825 GACGC GACGC(5/10)
HhaI 3066 GCGC GCG^C
Hin1I 2825 GACGCC G[GA]^CG[CT]C
HinP1I 3066 GCGC G^CGC
HincII 3332 GTCAAC GT[CT]^[GA]AC
HindII 3332 GTCAAC GT[CT]^[GA]AC
HindIII 3489 AAGCTT A^AGCTT
MamI 2350 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 202 CAATTG C^AATTG
MunI 202 CAATTG C^AATTG
NcoI 153 CCATGG C^CATGG
NdeI 3496 CATATG CA^TATG
PmaCI 1140 CACGTG CAC^GTG
PmlI 1140 CACGTG CAC^GTG
PshAI 2825 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1855 ATTAAT AT^TAAT
Psp1406I 1760 AACGTT AA^CGTT
PstI 972 CTGCAG CTGCA^G
RcaI 1554 TCATGA T^CATGA
ScaI 1055 AGTACT AGT^ACT
SwaI 1748 ATTTAAAT ATTT^AAAT
XcmI 3357 CCAGGCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 40 AAATTT [GA]^AATT[CT]
111 GAATTC
AhdI 997 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2410 GACATCTTGTC
AlwNI 3178 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3338 CAGTGGCTG
ApaI 3158 GGGCCC GGGCC^C
3584 GGGCCC
ApoI 40 AAATTT [GA]^AATT[CT]
111 GAATTC
Asp700I 440 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
668 GAAGCAATTC
AspEI 997 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2410 GACATCTTGTC
BpmI 1000 CTCCAG CTGGAG(16/14)
75 CTGGAG
BsaI 1046 GAGACC GGTCTC(1/5)
3044 GAGACC
BsmI 1219 GAATGC GAATGC(1/-1)
2775 GAATGC
BspLU11I 1303 ACATGT A^CATGT
2560 ACATGT
BspMI 2809 ACCTGC ACCTGC(4/8)
2368 GCAGGT
BsrDI 751 GCAATG GCAATG(2/0)
1208 GCAATG
BstEII 766 GGTGACC G^GT[ACGT]ACC
2053 GGTCACC
BstPI 766 GGTGACC G^GT[ACGT]ACC
2053 GGTCACC
DraI 468 TTTAAA TTT^AAA
1749 TTTAAA
DraIII 872 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
981 CACTGTGTG
DsaI 153 CCATGG C^C[GA][CT]GG
3078 CCACGG
Eam1105I 997 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2410 GACATCTTGTC
EcoO65I 766 GGTGACC G^GT[ACGT]ACC
2053 GGTCACC
EcoT22I 1486 ATGCAT ATGCA^T
2996 ATGCAT
HapII 1050 CCGG C^CGG
2691 CCGG
HpaII 1050 CCGG C^CGG
2691 CCGG
MspI 1050 CCGG C^CGG
2691 CCGG
NsiI 1486 ATGCAT ATGCA^T
2996 ATGCAT
PpuMI 126 GGGTCCT [GA]G^G[AT]CC[CT]
1251 AGGTCCC
SphI 2171 GCATGC GCATG^C
2998 GCATGC
StuI 1887 AGGCCT AGG^CCT
3244 AGGCCT
XmnI 440 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
668 GAAGCAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BalI BanII BbsI
BbvI BciVI BfaI BmyI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII