################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AflII AgeI Aor51HI AscI Asp718I AspI AvaI AviII AvrII BanI BbeI BbvCI BcgI BcnI BfrI BlnI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI EagI EarI EclXI Eco47III Eco52I EcoNI EcoRV FauI FseI FspI HaeII HpaI KasI KpnI Ksp632I KspI MluI MroI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AclI 1760 AACGTT AA^CGTT AcyI 2825 GACGCC G[GA]^CG[CT]C Alw44I 2758 GTGCAC G^TGCAC AocI 2877 CCTCAGG CC^T[ACGT]AGG ApaLI 2758 GTGCAC G^TGCAC AseI 1855 ATTAAT AT^TAAT AsnI 1855 ATTAAT AT^TAAT BamHI 1126 GGATCC G^GATCC BbrPI 1140 CACGTG CAC^GTG BclI 230 TGATCA T^GATCA BglI 3068 GCCACGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 555 AGATCT A^GATCT BlpI 1518 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1518 GCTCAGC GC^T[ACGT]AGC BsaAI 1140 CACGTG [CT]AC^GT[GA] BsaBI 2350 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2825 GACGCC G[GA]^CG[CT]C BsaWI 1049 ACCGGA [AT]^CCGG[AT] BsmBI 2158 CGTCTC CGTCTC(1/5) BspHI 1554 TCATGA T^CATGA BsrFI 2690 GCCGGT [GA]^CCGG[CT] BssSI 1799 CACGAG CACGAG(-5/-1) Bsu36I 2877 CCTCAGG CC^T[ACGT]AGG CelII 1518 GCTCAGC GC^T[ACGT]AGC CfoI 3066 GCGC GCG^C Cfr10I 2690 GCCGGT [GA]^CCGG[CT] DrdI 2424 GACTAGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2877 CCTCAGG CC^T[ACGT]AGG EcoRI 111 GAATTC G^AATTC FbaI 230 TGATCA T^GATCA HgaI 2825 GACGC GACGC(5/10) HhaI 3066 GCGC GCG^C Hin1I 2825 GACGCC G[GA]^CG[CT]C HinP1I 3066 GCGC G^CGC HincII 3332 GTCAAC GT[CT]^[GA]AC HindII 3332 GTCAAC GT[CT]^[GA]AC HindIII 3489 AAGCTT A^AGCTT MamI 2350 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 202 CAATTG C^AATTG MunI 202 CAATTG C^AATTG NcoI 153 CCATGG C^CATGG NdeI 3496 CATATG CA^TATG PmaCI 1140 CACGTG CAC^GTG PmlI 1140 CACGTG CAC^GTG PshAI 2825 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1855 ATTAAT AT^TAAT Psp1406I 1760 AACGTT AA^CGTT PstI 972 CTGCAG CTGCA^G RcaI 1554 TCATGA T^CATGA ScaI 1055 AGTACT AGT^ACT SwaI 1748 ATTTAAAT ATTT^AAAT XcmI 3357 CCAGGCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 40 AAATTT [GA]^AATT[CT] 111 GAATTC AhdI 997 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2410 GACATCTTGTC AlwNI 3178 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 3338 CAGTGGCTG ApaI 3158 GGGCCC GGGCC^C 3584 GGGCCC ApoI 40 AAATTT [GA]^AATT[CT] 111 GAATTC Asp700I 440 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 668 GAAGCAATTC AspEI 997 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2410 GACATCTTGTC BpmI 1000 CTCCAG CTGGAG(16/14) 75 CTGGAG BsaI 1046 GAGACC GGTCTC(1/5) 3044 GAGACC BsmI 1219 GAATGC GAATGC(1/-1) 2775 GAATGC BspLU11I 1303 ACATGT A^CATGT 2560 ACATGT BspMI 2809 ACCTGC ACCTGC(4/8) 2368 GCAGGT BsrDI 751 GCAATG GCAATG(2/0) 1208 GCAATG BstEII 766 GGTGACC G^GT[ACGT]ACC 2053 GGTCACC BstPI 766 GGTGACC G^GT[ACGT]ACC 2053 GGTCACC DraI 468 TTTAAA TTT^AAA 1749 TTTAAA DraIII 872 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG 981 CACTGTGTG DsaI 153 CCATGG C^C[GA][CT]GG 3078 CCACGG Eam1105I 997 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2410 GACATCTTGTC EcoO65I 766 GGTGACC G^GT[ACGT]ACC 2053 GGTCACC EcoT22I 1486 ATGCAT ATGCA^T 2996 ATGCAT HapII 1050 CCGG C^CGG 2691 CCGG HpaII 1050 CCGG C^CGG 2691 CCGG MspI 1050 CCGG C^CGG 2691 CCGG NsiI 1486 ATGCAT ATGCA^T 2996 ATGCAT PpuMI 126 GGGTCCT [GA]G^G[AT]CC[CT] 1251 AGGTCCC SphI 2171 GCATGC GCATG^C 2998 GCATGC StuI 1887 AGGCCT AGG^CCT 3244 AGGCCT XmnI 440 GAAATAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 668 GAAGCAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AspHI AvaII BalI BanII BbsI BbvI BciVI BfaI BmyI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII