################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AscI Asp718I AspEI AspI BamHI BbrPI BbvCI BcgI BfrI BsaBI BsaI BsaWI BseAI BsgI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssSI Bst1107I BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoRI EcoT22I FseI KpnI MamI MfeI MluI MroI MunI NdeI NheI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 2593 GTAGAC GT^[AC][GT]AC AflIII 2536 ACACGT A^C[GA][CT]GT Alw44I 745 GTGCAC G^TGCAC AocI 1239 CCTAAGG CC^T[ACGT]AGG Aor51HI 341 AGCGCT AGC^GCT ApaI 288 GGGCCC GGGCC^C ApaLI 745 GTGCAC G^TGCAC AseI 2227 ATTAAT AT^TAAT AsnI 2227 ATTAAT AT^TAAT AvaI 855 CTCGAG C^[CT]CG[GA]G AvrII 1600 CCTAGG C^CTAGG BciVI 2158 GGATAC GTATCC(6/5) BclI 2133 TGATCA T^GATCA BlnI 1600 CCTAGG C^CTAGG BsaAI 559 TACGTG [CT]AC^GT[GA] BsiEI 781 CGGCCG CG[GA][CT]^CG BsmFI 809 GGGAC GGGAC(10/14) BsmI 2519 GCATTC GAATGC(1/-1) BsoBI 855 CTCGAG C^[CT]CG[GA]G BsrBI 778 GAGCGG CCGCTC(-3/-3) BsrFI 269 GCCGGC [GA]^CCGG[CT] BssHII 517 GCGCGC G^CGCGC BstBI 1441 TTCGAA TT^CGAA BstXI 707 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1239 CCTAAGG CC^T[ACGT]AGG Cfr10I 269 GCCGGC [GA]^CCGG[CT] DrdI 1723 GACAGTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 781 CGGCCG C^GGCCG EclXI 781 CGGCCG C^GGCCG Eco47III 341 AGCGCT AGC^GCT Eco52I 781 CGGCCG C^GGCCG Eco81I 1239 CCTAAGG CC^T[ACGT]AGG EcoNI 1430 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2647 GATATC GAT^ATC FbaI 2133 TGATCA T^GATCA HindIII 1127 AAGCTT A^AGCTT HpaI 2389 GTTAAC GTT^AAC KspI 312 CCGCGG CCGC^GG NaeI 269 GCCGGC GCC^GGC NcoI 218 CCATGG C^CATGG NgoMIV 269 GCCGGC G^CCGGC NotI 780 GCGGCCGC GC^GGCCGC NspV 1441 TTCGAA TT^CGAA PaeR7I 855 CTCGAG C^TCGAG PshBI 2227 ATTAAT AT^TAAT PvuII 454 CAGCTG CAG^CTG SacII 312 CCGCGG CCGC^GG SfiI 954 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1441 TTCGAA TT^CGAA SphI 2747 GCATGC GCATG^C XhoI 855 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 1349 AAATTC [GA]^AATT[CT] 2140 AAATTC AlwNI 637 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1472 CAGTTACTG ApoI 1349 AAATTC [GA]^AATT[CT] 2140 AAATTC Asp700I 975 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1069 GAAGCCATTC AviII 383 TGCGCA TGC^GCA 563 TGCGCA BbsI 67 GAAGAC GAAGAC(2/6) 2677 GAAGAC BlpI 939 GCTCAGC GC^T[ACGT]AGC 1860 GCTCAGC Bpu1102I 939 GCTCAGC GC^T[ACGT]AGC 1860 GCTCAGC BpuAI 67 GAAGAC GAAGAC(2/6) 2677 GAAGAC BsmBI 613 CGTCTC CGTCTC(1/5) 240 GAGACG BsrDI 1659 CATTGC GCAATG(2/0) 1011 GCAATG BstAPI 636 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 747 GCACATGCTGC BstEII 39 GGTGACC G^GT[ACGT]ACC 483 GGTGACC BstPI 39 GGTGACC G^GT[ACGT]ACC 483 GGTGACC CelII 939 GCTCAGC GC^T[ACGT]AGC 1860 GCTCAGC EarI 2470 CTCTTC CTCTTC(1/4) 1060 GAAGAG EcoO65I 39 GGTGACC G^GT[ACGT]ACC 483 GGTGACC FspI 383 TGCGCA TGC^GCA 563 TGCGCA Ksp632I 2470 CTCTTC CTCTTC(1/4) 1060 GAAGAG MslI 246 CATGACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 372 CATCCCAGTG PpuMI 914 AGGACCC [GA]G^G[AT]CC[CT] 1596 AGGACCT SacI 937 GAGCTC GAGCT^C 1582 GAGCTC SspI 2091 AATATT AAT^ATT 2152 AATATT StuI 1236 AGGCCT AGG^CCT 1836 AGGCCT XmnI 975 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1069 GAAGCCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AspHI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII