################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI TseI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AfaI 3 GTAC GT^AC AflII 297 CTTAAG C^TTAAG Alw44I 59 GTGCAC G^TGCAC AlwI 352 GGATC GGATC(4/5) ApaLI 59 GTGCAC G^TGCAC AseI 142 ATTAAT AT^TAAT AsnI 142 ATTAAT AT^TAAT AspHI 59 GTGCAC G[AT]GC[AT]^C BfaI 235 CTAG C^TAG BfrI 297 CTTAAG C^TTAAG BmyI 59 GTGCAC G[AGT]GC[ACT]^C BsiHKAI 59 GTGCAC G[AT]GC[AT]^C BsmAI 388 GAGAC GTCTC(1/5) BsmFI 308 GGGAC GGGAC(10/14) Bsp1286I 59 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 2 TGTACA T^GTACA BsrDI 27 CATTGC GCAATG(2/0) BsrGI 2 TGTACA T^GTACA BsrI 99 ACTGG ACTGG(1/-1) BstYI 352 GGATCT [GA]^GATC[CT] DdeI 34 CTAAG C^T[ACGT]AG DpnII 353 GATC ^GATC HaeIII 207 GGCC GG^CC HinfI 156 GAATC G^A[ACGT]TC HphI 167 TCACC GGTGA(8/7) MaeI 235 CTAG C^TAG MaeII 329 ACGT A^CGT MaeIII 49 GTCAC ^GT[ACGT]AC MboI 353 GATC ^GATC MflI 352 GGATCT [GA]^GATC[CT] NdeII 353 GATC ^GATC NspI 194 ACATGC [GA]CATG^[CT] PshBI 142 ATTAAT AT^TAAT RsaI 3 GTAC GT^AC Sau3AI 353 GATC ^GATC SmlI 297 CTTAAG C^T[CT][GA]AG SspBI 2 TGTACA T^GTACA TfiI 156 GAATC G^A[AT]TC Tsp45I 49 GTCAC ^GT[GC]AC XbaI 234 TCTAGA T^CTAGA XhoII 352 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AcsI 270 AAATTT [GA]^AATT[CT] 406 GAATTT AluI 92 AGCT AG^CT 276 AGCT ApoI 270 AAATTT [GA]^AATT[CT] 406 GAATTT BpmI 100 CTGGAG CTGGAG(16/14) 256 CTGGAG BsaJI 304 CCCAGG C^C[ACGT][ACGT]GG 305 CCAGGG BssKI 170 CCAGG ^CC[ACGT]GG 305 CCAGG BstF5I 8 GGATG GGATG(2/0) 173 GGATG BstNI 170 CCAGG CC^[AT]GG 305 CCAGG EcoRII 170 CCAGG ^CC[AT]GG 305 CCAGG FokI 8 GGATG GGATG(9/13) 173 GGATG MnlI 253 CCTC CCTC(7/6) 259 GAGG MslI 168 CACCAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 365 CATTCATGTG MvaI 170 CCAGG CC^[AT]GG 305 CCAGG NlaIII 195 CATG CATG^ 369 CATG ScrFI 170 CCAGG CC^[ACGT]GG 305 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### MboII MseI SfaNI Tru9I Tsp509I TspRI