################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NlaIII NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AcsI 68 AAATTC [GA]^AATT[CT] AflIII 47 ACGTGT A^C[GA][CT]GT Alw44I 186 GTGCAC G^TGCAC ApaLI 186 GTGCAC G^TGCAC ApoI 68 AAATTC [GA]^AATT[CT] Asp700I 196 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 186 GTGCAC G[AT]GC[AT]^C BbvI 40 GCTGC GCAGC(8/12) BmyI 186 GTGCAC G[AGT]GC[ACT]^C BpmI 220 CTGGAG CTGGAG(16/14) BsaAI 46 TACGTG [CT]AC^GT[GA] BsaI 94 GAGACC GGTCTC(1/5) BsaJI 98 CCTTGG C^C[ACGT][ACGT]GG BsiHKAI 186 GTGCAC G[AT]GC[AT]^C Bsp1286I 186 GTGCAC G[AGT]GC[ACT]^C Bsp1407I 165 TGTACA T^GTACA BspMI 353 ACCTGC ACCTGC(4/8) BsrGI 165 TGTACA T^GTACA BsrI 219 ACTGG ACTGG(1/-1) Cac8I 27 GCTAGC GC[ACGT]^[ACGT]GC DdeI 148 CTGAG C^T[ACGT]AG DraIII 381 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 98 CCTTGG C^C[AT][AT]GG Fnu4HI 40 GCTGC GC^[ACGT]GC ItaI 40 GCTGC GC^[ACGT]GC MaeII 47 ACGT A^CGT MseI 277 TTAA T^TAA MwoI 31 GCTCTTGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 27 GCTAGC G^CTAGC SfaNI 6 GATGC GCATC(5/9) SspBI 165 TGTACA T^GTACA StyI 98 CCTTGG C^C[AT][AT]GG TaqI 120 TCGA T^CGA TfiI 153 GATTC G^A[AT]TC Tru9I 277 TTAA T^TAA TseI 40 GCTGC G^C[AT]GC TthHB8I 120 TCGA T^CGA XcmI 74 CCACAAGTGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 196 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AfaI 166 GTAC GT^AC 245 GTAC AluI 30 AGCT AG^CT 39 AGCT BfaI 28 CTAG C^TAG 346 CTAG BsmAI 94 GAGAC GTCTC(1/5) 142 GAGAC BstF5I 57 CATCC GGATG(2/0) 5 GGATG FokI 57 CATCC GGATG(9/13) 5 GGATG HphI 87 GGTGA GGTGA(8/7) 177 TCACC MaeI 28 CTAG C^TAG 346 CTAG MboII 193 GAAGA GAAGA(8/7) 319 GAAGA MnlI 3 GAGG CCTC(7/6) 150 GAGG PleI 144 GACTC GAGTC(4/5) 225 GAGTC RsaI 166 GTAC GT^AC 245 GTAC Tsp509I 69 AATT ^AATT 341 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### HinfI TspRI