##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       Hin1I       HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   14 enzymes) #
###################################

AfaI          161  GTAC                 GT^AC
AocI          672  CCTGAGG              CC^T[ACGT]AGG
Asp700I       100  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BpmI          499  CTGGAG               CTGGAG(16/14)
BsaJI         269  CCCTGG               C^C[ACGT][ACGT]GG
BsmI          104  GCATTC               GAATGC(1/-1)
Bsu36I        672  CCTGAGG              CC^T[ACGT]AGG
CfoI          669  GCGC                 GCG^C
Eco81I        672  CCTGAGG              CC^T[ACGT]AGG
HhaI          669  GCGC                 GCG^C
HinP1I        669  GCGC                 G^CGC
RsaI          161  GTAC                 GT^AC
SmlI           43  CTTGAG               C^T[CT][GA]AG
XmnI          100  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AciI          387  CCGC                 CCGC(-3/-1)
              615  CCGC                
BbsI          243  GAAGAC               GAAGAC(2/6)
              471  GAAGAC              
BpuAI         243  GAAGAC               GAAGAC(2/6)
              471  GAAGAC              
BstXI         263  CCATCACCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              491  CCATCACTCTGG        
HgaI          213  GCGTC                GACGC(5/10)
              441  GCGTC               
HphI           38  TCACC                GGTGA(8/7)
              266  TCACC               
SfaNI         332  GCATC                GCATC(5/9)
              560  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccI        AluI        AlwI        AvaII       BanII       
BbvI        BglII       BmyI        BsgI        BsiYI       
BslI        Bsp1286I    BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr10I      
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HincII      
HindII      HinfI       HpaII       ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        NdeII       NlaIV       PleI        
PvuII       SalI        Sau3AI      Sau96I      ScrFI       
SexAI       TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII