Restriction map for
pF1KE2061
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I AvrII BamHI BciVI BfrI
BglII BlnI BsaBI BsaWI BseAI
BseRI BsgI BsiWI Bsp1407I BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I CpoI DraI
DraIII EcoRV EcoT22I FseI HindIII
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 655 GTCTAC GT^[AC][GT]AC
AflIII 1073 ACACGT A^C[GA][CT]GT
AhdI 1575 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2082 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1119 AGCGCT AGC^GCT
AspEI 1575 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1305 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1108 TGCGCA TGC^GCA
BbrPI 1074 CACGTG CAC^GTG
BbvCI 1535 CCTCAGC CCTCAGC(-5/-2)
BcgI 1854 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 741 TGATCA T^GATCA
BfaI 197 CTAG C^TAG
BsaAI 1074 CACGTG [CT]AC^GT[GA]
BsiEI 188 CGGCCG CG[GA][CT]^CG
BsmI 648 GCATTC GAATGC(1/-1)
BspDI 922 ATCGAT AT^CGAT
BsrBI 1177 CCGCTC CCGCTC(-3/-3)
BsrDI 1439 CATTGC GCAATG(2/0)
BsrFI 1792 GCCGGC [GA]^CCGG[CT]
BssSI 1225 CTCGTG CACGAG(-5/-1)
BstEII 1186 GGTGACC G^GT[ACGT]ACC
BstPI 1186 GGTGACC G^GT[ACGT]ACC
BstYI 401 AGATCC [GA]^GATC[CT]
Bsu36I 2082 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1792 GCCGGC [GA]^CCGG[CT]
ClaI 922 ATCGAT AT^CGAT
EagI 188 CGGCCG C^GGCCG
Eam1105I 1575 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2104 CTCTTC CTCTTC(1/4)
EclXI 188 CGGCCG C^GGCCG
Eco47III 1119 AGCGCT AGC^GCT
Eco52I 188 CGGCCG C^GGCCG
Eco81I 2082 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1186 GGTGACC G^GT[ACGT]ACC
FbaI 741 TGATCA T^GATCA
FspI 1108 TGCGCA TGC^GCA
HpaI 637 GTTAAC GTT^AAC
Ksp632I 2104 CTCTTC CTCTTC(1/4)
KspI 321 CCGCGG CCGC^GG
MaeI 197 CTAG C^TAG
MflI 401 AGATCC [GA]^GATC[CT]
NaeI 1792 GCCGGC GCC^GGC
NgoMIV 1792 GCCGGC G^CCGGC
PflFI 1305 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1305 GACTC GAGTC(4/5)
PmaCI 1074 CACGTG CAC^GTG
PmlI 1074 CACGTG CAC^GTG
PshAI 1698 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1222 GAGCTC GAGCT^C
SacII 321 CCGCGG CCGC^GG
Tth111I 1305 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 401 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 984 GACGTC GACGT^C
1889 GACGTC
AcsI 439 GAATTC [GA]^AATT[CT]
727 GAATTC
AlwNI 1246 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1990 CAGTCTCTG
ApoI 439 GAATTC [GA]^AATT[CT]
727 GAATTC
Asp700I 496 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2088 GAAGTCTTTC
AspHI 1077 GTGCTC G[AT]GC[AT]^C
1222 GAGCTC
BalI 41 TGGCCA TGG^CCA
1299 TGGCCA
BbeI 14 GGCGCC GGCGC^C
1623 GGCGCC
BbsI 1005 GAAGAC GAAGAC(2/6)
1201 GAAGAC
BlpI 774 GCTCAGC GC^T[ACGT]AGC
1369 GCTCAGC
Bpu1102I 774 GCTCAGC GC^T[ACGT]AGC
1369 GCTCAGC
BpuAI 1005 GAAGAC GAAGAC(2/6)
1201 GAAGAC
BsaI 2068 GAGACC GGTCTC(1/5)
527 GGTCTC
BsiHKAI 1077 GTGCTC G[AT]GC[AT]^C
1222 GAGCTC
BsmBI 1655 CGTCTC CGTCTC(1/5)
1704 CGTCTC
BstXI 465 CCATGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
948 CCAGAAGATTGG
CelII 774 GCTCAGC GC^T[ACGT]AGC
1369 GCTCAGC
DrdI 937 GACGTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
984 GACGTCAGTGTC
EcoNI 974 CCTACATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2043 CCTTAGCCAGG
EcoRI 439 GAATTC G^AATTC
727 GAATTC
HgaI 78 GCGTC GACGC(5/10)
1555 GCGTC
KasI 14 GGCGCC G^GCGCC
1623 GGCGCC
MaeIII 668 GTGAC ^GT[ACGT]AC
1187 GTGAC
MluNI 41 TGGCCA TGG^CCA
1299 TGGCCA
MscI 41 TGGCCA TGG^CCA
1299 TGGCCA
MseI 422 TTAA T^TAA
638 TTAA
NarI 14 GGCGCC GG^CGCC
1623 GGCGCC
NcoI 405 CCATGG C^CATGG
1296 CCATGG
SfoI 14 GGCGCC GGC^GCC
1623 GGCGCC
TfiI 479 GATTC G^A[AT]TC
626 GAATC
Tru9I 422 TTAA T^TAA
638 TTAA
Tsp45I 668 GTGAC ^GT[GC]AC
1187 GTGAC
XmnI 496 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2088 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI StuI StyI TaqI TseI
Tsp509I TspRI TthHB8I XcmI XmaCI
XmaI