Restriction map for
pF1KE5572
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BfaI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FauI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 7 CGCG CG^CG
AciI 314 GCGG CCGC(-3/-1)
AlwI 1045 GGATC GGATC(4/5)
AvaI 674 CTCGGG C^[CT]CG[GA]G
BciVI 1339 GTATCC GTATCC(6/5)
BcnI 1343 CCCGG CC^[GC]GG
BsaBI 277 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 853 GAGGAG GAGGAG(10/8)
BsiYI 714 CCCTTGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 714 CCCTTGGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 748 GCATTC GAATGC(1/-1)
BsoBI 674 CTCGGG C^[CT]CG[GA]G
Bsp1407I 82 TGTACA T^GTACA
BspHI 99 TCATGA T^CATGA
BsrGI 82 TGTACA T^GTACA
BssSI 221 CACGAG CACGAG(-5/-1)
BstBI 890 TTCGAA TT^CGAA
BstF5I 327 GGATG GGATG(2/0)
BstUI 7 CGCG CG^CG
BstYI 1045 GGATCT [GA]^GATC[CT]
CfoI 6 GCGC GCG^C
DraI 306 TTTAAA TTT^AAA
DraII 1236 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 127 CGGCCA [CT]^GGCC[GA]
EcoO109I 1236 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 746 ATGCAT ATGCA^T
FokI 327 GGATG GGATG(9/13)
HapII 1344 CCGG C^CGG
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
HpaII 1344 CCGG C^CGG
MaeII 989 ACGT A^CGT
MamI 277 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1045 GGATCT [GA]^GATC[CT]
MspI 1344 CCGG C^CGG
MvnI 7 CGCG CG^CG
MwoI 314 GCGGCAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1343 CCCGG CC^[GC]GG
NsiI 746 ATGCAT ATGCA^T
NspV 890 TTCGAA TT^CGAA
PpuMI 1236 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 99 TCATGA T^CATGA
SexAI 702 ACCAGGT A^CC[AT]GGT
SfuI 890 TTCGAA TT^CGAA
SmlI 178 CTTGAG C^T[CT][GA]AG
SspBI 82 TGTACA T^GTACA
SspI 847 AATATT AAT^ATT
StuI 36 AGGCCT AGG^CCT
XhoII 1045 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 334 ATTAAT AT^TAAT
925 ATTAAT
AsnI 334 ATTAAT AT^TAAT
925 ATTAAT
Asp700I 536 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1026 GAACATCTTC
AvaII 1237 GGACC G^G[AT]CC
1262 GGACC
BbsI 1075 GTCTTC GAAGAC(2/6)
1145 GTCTTC
BclI 17 TGATCA T^GATCA
96 TGATCA
BpuAI 1075 GTCTTC GAAGAC(2/6)
1145 GTCTTC
BsaJI 715 CCTTGG C^C[ACGT][ACGT]GG
1334 CCAAGG
BsmAI 577 GAGAC GTCTC(1/5)
856 GAGAC
BspMI 720 GCAGGT ACCTGC(4/8)
835 GCAGGT
BstNI 34 CCAGG CC^[AT]GG
703 CCAGG
Cac8I 422 GCGAGC GC[ACGT]^[ACGT]GC
1189 GCTGGC
Cfr13I 1237 GGACC G^G[ACGT]CC
1262 GGACC
EcoRII 34 CCAGG ^CC[AT]GG
703 CCAGG
EcoRV 62 GATATC GAT^ATC
479 GATATC
EcoT14I 715 CCTTGG C^C[AT][AT]GG
1334 CCAAGG
FbaI 17 TGATCA T^GATCA
96 TGATCA
HaeIII 37 GGCC GG^CC
128 GGCC
HincII 1129 GTTGAC GT[CT]^[GA]AC
1351 GTCAAC
HindII 1129 GTTGAC GT[CT]^[GA]AC
1351 GTCAAC
MslI 15 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
524 CATTTCCATG
MvaI 34 CCAGG CC^[AT]GG
703 CCAGG
PleI 292 GAGTC GAGTC(4/5)
1073 GAGTC
PshBI 334 ATTAAT AT^TAAT
925 ATTAAT
Sau96I 1237 GGACC G^G[ACGT]CC
1262 GGACC
StyI 715 CCTTGG C^C[AT][AT]GG
1334 CCAAGG
XmnI 536 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1026 GAACATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
BbvI BsmFI BspLU11I BsrDI BsrI
BssKI DdeI DpnII EarI Fnu4HI
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
ScrFI SfaNI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I