Restriction map for
pF1KE2032
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AflII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbrPI BbsI BcgI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsiWI Bsp1407I BspDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eco47III Eco81I
EcoRI EcoT22I FseI HincII HindII
HpaI KpnI KspI MaeI MfeI
MluI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SalI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccIII 1145 TCCGGA T^CCGGA
AclI 1540 AACGTT AA^CGTT
AcsI 462 GAATTT [GA]^AATT[CT]
AhdI 1709 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 64 GTGCAC G^TGCAC
AlwNI 1159 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 64 GTGCAC G^TGCAC
ApoI 462 GAATTT [GA]^AATT[CT]
AspEI 1709 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 558 TGGCCA TGG^CCA
BamHI 1622 GGATCC G^GATCC
BanI 668 GGCGCC G^G[CT][GA]CC
BbeI 668 GGCGCC GGCGC^C
BclI 1851 TGATCA T^GATCA
BglI 208 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1337 AGATCT A^GATCT
BsaWI 1145 TCCGGA [AT]^CCGG[AT]
BseAI 1145 TCCGGA T^CCGGA
BsmI 538 GAATGC GAATGC(1/-1)
BspEI 1145 TCCGGA T^CCGGA
BspHI 1718 TCATGA T^CATGA
BsrBI 1594 GAGCGG CCGCTC(-3/-3)
BsrDI 1386 CATTGC GCAATG(2/0)
BsrFI 1599 GCCGGT [GA]^CCGG[CT]
BstEII 678 GGTGACC G^GT[ACGT]ACC
BstPI 678 GGTGACC G^GT[ACGT]ACC
Cfr10I 1599 GCCGGT [GA]^CCGG[CT]
DraIII 1435 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1192 GACCGCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1597 CGGCCG C^GGCCG
Eam1105I 1709 GACCCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1597 CGGCCG C^GGCCG
Eco52I 1597 CGGCCG C^GGCCG
EcoNI 1663 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 678 GGTGACC G^GT[ACGT]ACC
EcoRV 1320 GATATC GAT^ATC
FbaI 1851 TGATCA T^GATCA
HaeII 668 GGCGCC [GA]GCGC^[CT]
HgaI 282 GACGC GACGC(5/10)
HindIII 295 AAGCTT A^AGCTT
KasI 668 GGCGCC G^GCGCC
MluNI 558 TGGCCA TGG^CCA
MroI 1145 TCCGGA T^CCGGA
MscI 558 TGGCCA TGG^CCA
MseI 1733 TTAA T^TAA
MslI 1809 CACACATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 668 GGCGCC GG^CGCC
PaeR7I 287 CTCGAG C^TCGAG
Psp1406I 1540 AACGTT AA^CGTT
PstI 1567 CTGCAG CTGCA^G
RcaI 1718 TCATGA T^CATGA
SacI 1060 GAGCTC GAGCT^C
SapI 1722 GAAGAGC GCTCTTC(1/4)
SbfI 1566 CCTGCAGG CCTGCA^GG
SexAI 1294 ACCAGGT A^CC[AT]GGT
SfoI 668 GGCGCC GGC^GCC
SmlI 287 CTCGAG C^T[CT][GA]AG
Sse8387I 1566 CCTGCAGG CCTGCA^GG
StuI 206 AGGCCT AGG^CCT
Tru9I 1733 TTAA T^TAA
Tsp509I 463 AATT ^AATT
XcmI 131 CCAACACCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 287 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 282 GACGCC G[GA]^CG[CT]C
668 GGCGCC
AflIII 1640 ACATGT A^C[GA][CT]GT
1812 ACATGT
AspHI 64 GTGCAC G[AT]GC[AT]^C
1060 GAGCTC
AvaI 287 CTCGAG C^[CT]CG[GA]G
990 CTCGGG
AviII 152 TGCGCA TGC^GCA
1271 TGCGCA
BbvCI 91 CCTCAGC CCTCAGC(-5/-2)
325 GCTGAGG
BciVI 1486 GGATAC GTATCC(6/5)
1835 GGATAC
BsaBI 1338 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1623 GATCCGCATC
BsaHI 282 GACGCC G[GA]^CG[CT]C
668 GGCGCC
BsgI 192 GTGCAG GTGCAG(16/14)
763 GTGCAG
BsiEI 103 CGGTCG CG[GA][CT]^CG
1597 CGGCCG
BsiHKAI 64 GTGCAC G[AT]GC[AT]^C
1060 GAGCTC
BsoBI 287 CTCGAG C^[CT]CG[GA]G
990 CTCGGG
BspLU11I 1640 ACATGT A^CATGT
1812 ACATGT
BstAPI 377 GCATTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1131 GCAGGTGATGC
BstYI 1337 AGATCT [GA]^GATC[CT]
1622 GGATCC
EarI 1149 GAAGAG CTCTTC(1/4)
1722 GAAGAG
EcoT14I 725 CCAAGG C^C[AT][AT]GG
1126 CCTTGG
FspI 152 TGCGCA TGC^GCA
1271 TGCGCA
Hin1I 282 GACGCC G[GA]^CG[CT]C
668 GGCGCC
Ksp632I 1149 GAAGAG CTCTTC(1/4)
1722 GAAGAG
MamI 1338 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1623 GATCCGCATC
MflI 1337 AGATCT [GA]^GATC[CT]
1622 GGATCC
StyI 725 CCAAGG C^C[AT][AT]GG
1126 CCTTGG
TfiI 612 GAATC G^A[AT]TC
1069 GAATC
XhoII 1337 AGATCT [GA]^GATC[CT]
1622 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TspRI TthHB8I