Restriction map for
pF1KE2321
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AccI AclI AgeI Aor51HI
AscI AseI AsnI Asp718I AviII
AvrII BcgI BlnI BspDI BssHII
Bst1107I BstZ17I ClaI CpoI DraI
Eco47III EcoRI FspI HpaI KpnI
KspI MfeI MluI MunI NdeI
NotI NruI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SnaBI
SpeI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 2065 TCCGGA T^CCGGA
AcsI 2473 AAATTC [GA]^AATT[CT]
AflII 967 CTTAAG C^TTAAG
AhdI 733 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2473 AAATTC [GA]^AATT[CT]
Asp700I 2811 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 733 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 775 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 148 GGCGCC GGCGC^C
BbrPI 274 CACGTG CAC^GTG
BciVI 3665 GGATAC GTATCC(6/5)
BfrI 967 CTTAAG C^TTAAG
BlpI 636 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 636 GCTGAGC GC^T[ACGT]AGC
BsaBI 1911 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2065 TCCGGA T^CCGGA
BsiWI 4217 CGTACG C^GTACG
Bsp1407I 3938 TGTACA T^GTACA
BspEI 2065 TCCGGA T^CCGGA
BspHI 4109 TCATGA T^CATGA
BsrGI 3938 TGTACA T^GTACA
BstAPI 3381 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2125 TTCGAA TT^CGAA
BstEII 2427 GGTCACC G^GT[ACGT]ACC
BstPI 2427 GGTCACC G^GT[ACGT]ACC
CelII 636 GCTGAGC GC^T[ACGT]AGC
DraIII 2704 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 733 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2427 GGTCACC G^GT[ACGT]ACC
EcoRV 3811 GATATC GAT^ATC
FseI 205 GGCCGGCC GGCCGG^CC
KasI 148 GGCGCC G^GCGCC
MamI 1911 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2065 TCCGGA T^CCGGA
NarI 148 GGCGCC GG^CGCC
NheI 2214 GCTAGC G^CTAGC
NspV 2125 TTCGAA TT^CGAA
PaeR7I 3611 CTCGAG C^TCGAG
PflFI 775 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1896 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 274 CACGTG CAC^GTG
PmlI 274 CACGTG CAC^GTG
RcaI 4109 TCATGA T^CATGA
ScaI 3299 AGTACT AGT^ACT
SfoI 148 GGCGCC GGC^GCC
SfuI 2125 TTCGAA TT^CGAA
SgrAI 4143 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 3938 TGTACA T^GTACA
Tth111I 775 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1896 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3611 CTCGAG C^TCGAG
XmnI 2811 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 11 ACATGT A^C[GA][CT]GT
3560 ACATGT
Alw44I 2395 GTGCAC G^TGCAC
3577 GTGCAC
AocI 860 CCTCAGG CC^T[ACGT]AGG
1377 CCTCAGG
ApaI 294 GGGCCC GGGCC^C
2596 GGGCCC
ApaLI 2395 GTGCAC G^TGCAC
3577 GTGCAC
BamHI 1268 GGATCC G^GATCC
2744 GGATCC
BbvCI 475 CCTCAGC CCTCAGC(-5/-2)
3396 CCTCAGC
BclI 122 TGATCA T^GATCA
1101 TGATCA
BglI 584 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2018 GCCGGGGGGGC
BglII 2339 AGATCT A^GATCT
4240 AGATCT
BsaAI 274 CACGTG [CT]AC^GT[GA]
3979 TACGTG
BsaWI 2065 TCCGGA [AT]^CCGG[AT]
3662 ACCGGA
BsmI 1245 GAATGC GAATGC(1/-1)
3147 GAATGC
BspLU11I 11 ACATGT A^CATGT
3560 ACATGT
Bsu36I 860 CCTCAGG CC^T[ACGT]AGG
1377 CCTCAGG
EagI 204 CGGCCG C^GGCCG
419 CGGCCG
EclXI 204 CGGCCG C^GGCCG
419 CGGCCG
Eco52I 204 CGGCCG C^GGCCG
419 CGGCCG
Eco81I 860 CCTCAGG CC^T[ACGT]AGG
1377 CCTCAGG
EcoT22I 1021 ATGCAT ATGCA^T
1527 ATGCAT
FbaI 122 TGATCA T^GATCA
1101 TGATCA
HindIII 2315 AAGCTT A^AGCTT
3786 AAGCTT
NsiI 1021 ATGCAT ATGCA^T
1527 ATGCAT
SbfI 3903 CCTGCAGG CCTGCA^GG
3993 CCTGCAGG
SmaI 463 CCCGGG CCC^GGG
3953 CCCGGG
SphI 480 GCATGC GCATG^C
1035 GCATGC
Sse8387I 3903 CCTGCAGG CCTGCA^GG
3993 CCTGCAGG
StuI 1045 AGGCCT AGG^CCT
3290 AGGCCT
XcmI 386 CCACCAAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1136 CCAAAGCCCACATGG
XmaCI 463 CCCGGG C^CCGGG
3953 CCCGGG
XmaI 463 CCCGGG C^CCGGG
3953 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII