Restriction map for
pF1KB9878
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwNI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstZ17I
CelII ClaI CpoI DraI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FspI HindIII HpaI KasI MamI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SexAI SfiI SfoI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 804 GACGTC GACGT^C
Acc65I 1030 GGTACC G^GTACC
AccI 1246 GTCTAC GT^[AC][GT]AC
AocI 1086 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1618 AGCGCT AGC^GCT
ApaI 212 GGGCCC GGGCC^C
Asp718I 1030 GGTACC G^GTACC
BbrPI 1501 CACGTG CAC^GTG
BcgI 704 GCATCGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 953 CTAG C^TAG
BsaAI 1501 CACGTG [CT]AC^GT[GA]
BsgI 1665 CTGCAC GTGCAG(16/14)
BspHI 980 TCATGA T^CATGA
BstEII 1317 GGTAACC G^GT[ACGT]ACC
BstPI 1317 GGTAACC G^GT[ACGT]ACC
BstXI 1070 CCATGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1086 CCTGAGG CC^T[ACGT]AGG
DraIII 1351 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1618 AGCGCT AGC^GCT
Eco81I 1086 CCTGAGG CC^T[ACGT]AGG
EcoNI 609 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1317 GGTAACC G^GT[ACGT]ACC
FseI 692 GGCCGGCC GGCCGG^CC
KpnI 1030 GGTACC GGTAC^C
KspI 284 CCGCGG CCGC^GG
MaeI 953 CTAG C^TAG
NcoI 578 CCATGG C^CATGG
NruI 970 TCGCGA TCG^CGA
NspI 1496 ACATGC [GA]CATG^[CT]
PmaCI 1501 CACGTG CAC^GTG
PmlI 1501 CACGTG CAC^GTG
PpuMI 847 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1150 CTGCAG CTGCA^G
RcaI 980 TCATGA T^CATGA
SacII 284 CCGCGG CCGC^GG
ScaI 437 AGTACT AGT^ACT
SfcI 1150 CTGCAG C^T[GA][CT]AG
SgrAI 1398 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 221 CTCAAG C^T[CT][GA]AG
Tsp45I 1357 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AspHI 684 GAGCTC G[AT]GC[AT]^C
1540 GAGCTC
AvaII 174 GGACC G^G[AT]CC
848 GGTCC
BalI 1001 TGGCCA TGG^CCA
1079 TGGCCA
BbsI 357 GAAGAC GAAGAC(2/6)
1111 GTCTTC
BpuAI 357 GAAGAC GAAGAC(2/6)
1111 GTCTTC
BsiHKAI 684 GAGCTC G[AT]GC[AT]^C
1540 GAGCTC
BsmFI 173 GGGAC GGGAC(10/14)
849 GTCCC
BspMI 179 ACCTGC ACCTGC(4/8)
1663 ACCTGC
BsrBI 424 CCGCTC CCGCTC(-3/-3)
622 GAGCGG
BsrDI 1418 CATTGC GCAATG(2/0)
1055 GCAATG
BstBI 820 TTCGAA TT^CGAA
922 TTCGAA
EagI 281 CGGCCG C^GGCCG
511 CGGCCG
EclXI 281 CGGCCG C^GGCCG
511 CGGCCG
Eco52I 281 CGGCCG C^GGCCG
511 CGGCCG
EcoT14I 578 CCATGG C^C[AT][AT]GG
1142 CCAAGG
HincII 517 GTCAAC GT[CT]^[GA]AC
1696 GTCAAC
HindII 517 GTCAAC GT[CT]^[GA]AC
1696 GTCAAC
HinfI 814 GACTC G^A[ACGT]TC
1566 GACTC
MluNI 1001 TGGCCA TGG^CCA
1079 TGGCCA
MscI 1001 TGGCCA TGG^CCA
1079 TGGCCA
NspV 820 TTCGAA TT^CGAA
922 TTCGAA
PflMI 96 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
177 CCACCTGCTGG
PleI 814 GACTC GAGTC(4/5)
1566 GACTC
PvuII 352 CAGCTG CAG^CTG
562 CAGCTG
SacI 684 GAGCTC GAGCT^C
1540 GAGCTC
SapI 1480 GAAGAGC GCTCTTC(1/4)
666 GCTCTTC
SfuI 820 TTCGAA TT^CGAA
922 TTCGAA
SmaI 169 CCCGGG CCC^GGG
698 CCCGGG
StyI 578 CCATGG C^C[AT][AT]GG
1142 CCAAGG
Tsp509I 1450 AATT ^AATT
1651 AATT
Van91I 96 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
177 CCACCTGCTGG
XcmI 1181 CCATGCGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1322 CCATGACAACAGTGG
XmaCI 169 CCCGGG C^CCGGG
698 CCCGGG
XmaI 169 CCCGGG C^CCGGG
698 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI BanI BanII BbvCI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmBI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TspRI TthHB8I XhoII