Restriction map for
pF1KE2400
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HindIII HpaI KasI KpnI
KspI MluI MroI MspA1I NarI
NcoI NdeI NheI NotI NruI
NsiI NspI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 914 GACGTC GACGT^C
AccII 868 CGCG CG^CG
AciI 1896 GCGG CCGC(-3/-1)
AcyI 914 GACGTC G[GA]^CG[CT]C
AflIII 1204 ACACGT A^C[GA][CT]GT
Aor51HI 329 AGCGCT AGC^GCT
AseI 1444 ATTAAT AT^TAAT
AsnI 1444 ATTAAT AT^TAAT
Asp700I 211 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 583 CCCGGG C^[CT]CG[GA]G
AvaII 1453 GGTCC G^G[AT]CC
BalI 1469 TGGCCA TGG^CCA
BanI 1962 GGTGCC G^G[CT][GA]CC
BbsI 1349 GTCTTC GAAGAC(2/6)
BcgI 1955 CGATCAAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1349 GTCTTC GAAGAC(2/6)
BsaBI 1821 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 914 GACGTC G[GA]^CG[CT]C
BsmBI 45 GAGACG CGTCTC(1/5)
BsmFI 1683 GGGAC GGGAC(10/14)
BsoBI 583 CCCGGG C^[CT]CG[GA]G
Bsp1407I 258 TGTACA T^GTACA
BspMI 787 ACCTGC ACCTGC(4/8)
BsrDI 134 GCAATG GCAATG(2/0)
BsrFI 1151 GCCGGC [GA]^CCGG[CT]
BsrGI 258 TGTACA T^GTACA
BssSI 770 CTCGTG CACGAG(-5/-1)
BstAPI 782 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 427 TTCGAA TT^CGAA
BstUI 868 CGCG CG^CG
CfoI 330 GCGC GCG^C
Cfr10I 1151 GCCGGC [GA]^CCGG[CT]
DraI 565 TTTAAA TTT^AAA
DsaI 2065 CCACGG C^C[GA][CT]GG
EaeI 1469 TGGCCA [CT]^GGCC[GA]
Eco47III 329 AGCGCT AGC^GCT
HaeII 329 AGCGCT [GA]GCGC^[CT]
HhaI 330 GCGC GCG^C
Hin1I 914 GACGTC G[GA]^CG[CT]C
HinP1I 330 GCGC G^CGC
HincII 1084 GTCAAC GT[CT]^[GA]AC
HindII 1084 GTCAAC GT[CT]^[GA]AC
MamI 1821 GATCATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1469 TGGCCA TGG^CCA
MscI 1469 TGGCCA TGG^CCA
MvnI 868 CGCG CG^CG
NaeI 1151 GCCGGC GCC^GGC
NgoMIV 1151 GCCGGC G^CCGGC
NlaIV 1962 GGTGCC GG[ACGT]^[ACGT]CC
NspV 427 TTCGAA TT^CGAA
PshBI 1444 ATTAAT AT^TAAT
PstI 944 CTGCAG CTGCA^G
SapI 1867 GCTCTTC GCTCTTC(1/4)
ScaI 1611 AGTACT AGT^ACT
SfuI 427 TTCGAA TT^CGAA
SmaI 583 CCCGGG CCC^GGG
SmlI 1216 CTTGAG C^T[CT][GA]AG
SspBI 258 TGTACA T^GTACA
SspI 1038 AATATT AAT^ATT
StuI 75 AGGCCT AGG^CCT
XmaCI 583 CCCGGG C^CCGGG
XmaI 583 CCCGGG C^CCGGG
XmnI 211 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
BciVI 1923 GGATAC GTATCC(6/5)
1501 GTATCC
BmyI 1718 GGGCAC G[AGT]GC[ACT]^C
1963 GTGCCC
BsaI 61 GAGACC GGTCTC(1/5)
1930 GAGACC
BseRI 1219 GAGGAG GAGGAG(10/8)
1370 GAGGAG
BsmI 1863 GAATGC GAATGC(1/-1)
1991 GCATTC
Bsp1286I 1718 GGGCAC G[AGT]GC[ACT]^C
1963 GTGCCC
BstNI 57 CCTGG CC^[AT]GG
1266 CCTGG
BstYI 1139 AGATCC [GA]^GATC[CT]
1541 GGATCT
Cfr13I 1453 GGTCC G^G[ACGT]CC
1786 GGCCC
DraIII 711 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
1969 CACTCAGTG
EarI 1868 CTCTTC CTCTTC(1/4)
1079 GAAGAG
EcoRII 57 CCTGG ^CC[AT]GG
1266 CCTGG
EcoT14I 264 CCTTGG C^C[AT][AT]GG
515 CCTTGG
HgaI 1559 GACGC GACGC(5/10)
869 GCGTC
Ksp632I 1868 CTCTTC CTCTTC(1/4)
1079 GAAGAG
MaeII 915 ACGT A^CGT
1206 ACGT
MfeI 452 CAATTG C^AATTG
538 CAATTG
MflI 1139 AGATCC [GA]^GATC[CT]
1541 GGATCT
MunI 452 CAATTG C^AATTG
538 CAATTG
MvaI 57 CCTGG CC^[AT]GG
1266 CCTGG
PflMI 534 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCACTCAGTGG
Sau96I 1453 GGTCC G^G[ACGT]CC
1786 GGCCC
StyI 264 CCTTGG C^C[AT][AT]GG
515 CCTTGG
Tsp45I 1259 GTGAC ^GT[GC]AC
2086 GTGAC
Van91I 534 CCAACAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCACTCAGTGG
XhoII 1139 AGATCC [GA]^GATC[CT]
1541 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI BbvI BclI BcnI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BspHI BsrI BssKI BstF5I Cac8I
DdeI DpnII FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspI MwoI
NciI NdeII NlaIII PleI RcaI
RsaI Sau3AI ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I