Restriction map for
pF1KE2469
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# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccIII AflII AgeI Aor51HI AscI
AseI AsnI AviII AvrII BclI
BfrI BlnI BseAI BsiWI BspDI
BspEI BspLU11I BssHII Bst1107I BstZ17I
ClaI CpoI DraI Eco47III EcoRI
EcoT22I FbaI FseI FspI HpaI
MfeI MluI MroI MunI NdeI
NheI NruI NsiI PacI PinAI
PmeI PshBI PvuI RsrII SalI
SgrAI SnaBI SpeI SphI SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 994 GACGTC GACGT^C
Acc65I 2734 GGTACC G^GTACC
AccI 364 GTCTAC GT^[AC][GT]AC
AclI 2545 AACGTT AA^CGTT
AcsI 4440 GAATTT [GA]^AATT[CT]
AflIII 4190 ACACGT A^C[GA][CT]GT
AhdI 2950 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4440 GAATTT [GA]^AATT[CT]
Asp718I 2734 GGTACC G^GTACC
AspEI 2950 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 224 GGATCC G^GATCC
BbeI 890 GGCGCC GGCGC^C
BbrPI 2058 CACGTG CAC^GTG
BcgI 4220 GCATTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2105 GTATCC GTATCC(6/5)
BglII 1247 AGATCT A^GATCT
BsaAI 2058 CACGTG [CT]AC^GT[GA]
BsaWI 3308 ACCGGA [AT]^CCGG[AT]
BsmBI 1567 GAGACG CGTCTC(1/5)
BsmI 2761 GAATGC GAATGC(1/-1)
Bsp1407I 317 TGTACA T^GTACA
BsrGI 317 TGTACA T^GTACA
BstBI 4195 TTCGAA TT^CGAA
DraIII 4347 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2950 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 2498 GTCAAC GT[CT]^[GA]AC
HindII 2498 GTCAAC GT[CT]^[GA]AC
HindIII 3676 AAGCTT A^AGCTT
KasI 890 GGCGCC G^GCGCC
KpnI 2734 GGTACC GGTAC^C
KspI 1509 CCGCGG CCGC^GG
NarI 890 GGCGCC GG^CGCC
NotI 4133 GCGGCCGC GC^GGCCGC
NspV 4195 TTCGAA TT^CGAA
PaeR7I 2883 CTCGAG C^TCGAG
PmaCI 2058 CACGTG CAC^GTG
PmlI 2058 CACGTG CAC^GTG
PshAI 2646 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2545 AACGTT AA^CGTT
SacII 1509 CCGCGG CCGC^GG
SapI 243 GCTCTTC GCTCTTC(1/4)
SbfI 4275 CCTGCAGG CCTGCA^GG
SexAI 4640 ACCTGGT A^CC[AT]GGT
SfoI 890 GGCGCC GGC^GCC
SfuI 4195 TTCGAA TT^CGAA
Sse8387I 4275 CCTGCAGG CCTGCA^GG
SspBI 317 TGTACA T^GTACA
XhoI 2883 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
ApaI 773 GGGCCC GGGCC^C
1896 GGGCCC
AspI 4282 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4711 GACAGCGTC
BbvCI 900 CCTCAGC CCTCAGC(-5/-2)
3156 CCTCAGC
BlpI 1827 GCTAAGC GC^T[ACGT]AGC
4698 GCTCAGC
Bpu1102I 1827 GCTAAGC GC^T[ACGT]AGC
4698 GCTCAGC
BspHI 1634 TCATGA T^CATGA
2792 TCATGA
CelII 1827 GCTAAGC GC^T[ACGT]AGC
4698 GCTCAGC
DrdI 52 GACCAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3178 GACCAGACTGTC
EagI 35 CGGCCG C^GGCCG
4134 CGGCCG
EclXI 35 CGGCCG C^GGCCG
4134 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
4134 CGGCCG
EcoRV 2287 GATATC GAT^ATC
2581 GATATC
HaeII 890 GGCGCC [GA]GCGC^[CT]
3118 AGCGCC
NaeI 2611 GCCGGC GCC^GGC
3022 GCCGGC
NgoMIV 2611 GCCGGC G^CCGGC
3022 GCCGGC
NspI 320 ACATGC [GA]CATG^[CT]
3769 GCATGT
PflFI 4282 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4711 GACAGCGTC
RcaI 1634 TCATGA T^CATGA
2792 TCATGA
ScaI 1421 AGTACT AGT^ACT
3539 AGTACT
SfiI 1972 GGCCTCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3835 GGCCTGGTGGGCC
StuI 803 AGGCCT AGG^CCT
4407 AGGCCT
Tth111I 4282 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4711 GACAGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaBI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI