################################## # NO CUT ENZYMES ( 52 enzymes) # ################################## AccIII AflII AgeI Aor51HI AscI AseI AsnI AviII AvrII BclI BfrI BlnI BseAI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstZ17I ClaI CpoI DraI Eco47III EcoRI EcoT22I FbaI FseI FspI HpaI MfeI MluI MroI MunI NdeI NheI NruI NsiI PacI PinAI PmeI PshBI PvuI RsrII SalI SgrAI SnaBI SpeI SphI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 994 GACGTC GACGT^C Acc65I 2734 GGTACC G^GTACC AccI 364 GTCTAC GT^[AC][GT]AC AclI 2545 AACGTT AA^CGTT AcsI 4440 GAATTT [GA]^AATT[CT] AflIII 4190 ACACGT A^C[GA][CT]GT AhdI 2950 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 4440 GAATTT [GA]^AATT[CT] Asp718I 2734 GGTACC G^GTACC AspEI 2950 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 224 GGATCC G^GATCC BbeI 890 GGCGCC GGCGC^C BbrPI 2058 CACGTG CAC^GTG BcgI 4220 GCATTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2105 GTATCC GTATCC(6/5) BglII 1247 AGATCT A^GATCT BsaAI 2058 CACGTG [CT]AC^GT[GA] BsaWI 3308 ACCGGA [AT]^CCGG[AT] BsmBI 1567 GAGACG CGTCTC(1/5) BsmI 2761 GAATGC GAATGC(1/-1) Bsp1407I 317 TGTACA T^GTACA BsrGI 317 TGTACA T^GTACA BstBI 4195 TTCGAA TT^CGAA DraIII 4347 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2950 GACAACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HincII 2498 GTCAAC GT[CT]^[GA]AC HindII 2498 GTCAAC GT[CT]^[GA]AC HindIII 3676 AAGCTT A^AGCTT KasI 890 GGCGCC G^GCGCC KpnI 2734 GGTACC GGTAC^C KspI 1509 CCGCGG CCGC^GG NarI 890 GGCGCC GG^CGCC NotI 4133 GCGGCCGC GC^GGCCGC NspV 4195 TTCGAA TT^CGAA PaeR7I 2883 CTCGAG C^TCGAG PmaCI 2058 CACGTG CAC^GTG PmlI 2058 CACGTG CAC^GTG PshAI 2646 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2545 AACGTT AA^CGTT SacII 1509 CCGCGG CCGC^GG SapI 243 GCTCTTC GCTCTTC(1/4) SbfI 4275 CCTGCAGG CCTGCA^GG SexAI 4640 ACCTGGT A^CC[AT]GGT SfoI 890 GGCGCC GGC^GCC SfuI 4195 TTCGAA TT^CGAA Sse8387I 4275 CCTGCAGG CCTGCA^GG SspBI 317 TGTACA T^GTACA XhoI 2883 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### ApaI 773 GGGCCC GGGCC^C 1896 GGGCCC AspI 4282 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4711 GACAGCGTC BbvCI 900 CCTCAGC CCTCAGC(-5/-2) 3156 CCTCAGC BlpI 1827 GCTAAGC GC^T[ACGT]AGC 4698 GCTCAGC Bpu1102I 1827 GCTAAGC GC^T[ACGT]AGC 4698 GCTCAGC BspHI 1634 TCATGA T^CATGA 2792 TCATGA CelII 1827 GCTAAGC GC^T[ACGT]AGC 4698 GCTCAGC DrdI 52 GACCAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3178 GACCAGACTGTC EagI 35 CGGCCG C^GGCCG 4134 CGGCCG EclXI 35 CGGCCG C^GGCCG 4134 CGGCCG Eco52I 35 CGGCCG C^GGCCG 4134 CGGCCG EcoRV 2287 GATATC GAT^ATC 2581 GATATC HaeII 890 GGCGCC [GA]GCGC^[CT] 3118 AGCGCC NaeI 2611 GCCGGC GCC^GGC 3022 GCCGGC NgoMIV 2611 GCCGGC G^CCGGC 3022 GCCGGC NspI 320 ACATGC [GA]CATG^[CT] 3769 GCATGT PflFI 4282 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4711 GACAGCGTC RcaI 1634 TCATGA T^CATGA 2792 TCATGA ScaI 1421 AGTACT AGT^ACT 3539 AGTACT SfiI 1972 GGCCTCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3835 GGCCTGGTGGGCC StuI 803 AGGCCT AGG^CCT 4407 AGGCCT Tth111I 4282 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4711 GACAGCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI