################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AvrII BcgI BciVI BclI BfrI BlnI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HgaI HindIII HpaI KpnI MfeI MluI MseI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SbfI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I XbaI XhoI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccIII 1728 TCCGGA T^CCGGA Alw44I 2317 GTGCAC G^TGCAC ApaLI 2317 GTGCAC G^TGCAC Asp700I 2700 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BsaBI 141 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1872 GGTCTC GGTCTC(1/5) BsaWI 1728 TCCGGA [AT]^CCGG[AT] BseAI 1728 TCCGGA T^CCGGA BspEI 1728 TCCGGA T^CCGGA BsrDI 310 GCAATG GCAATG(2/0) BstXI 609 CCAGCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG HincII 1198 GTCGAC GT[CT]^[GA]AC HindII 1198 GTCGAC GT[CT]^[GA]AC MamI 141 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1728 TCCGGA T^CCGGA NheI 1656 GCTAGC G^CTAGC NspI 1787 ACATGC [GA]CATG^[CT] PflFI 777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SalI 1198 GTCGAC G^TCGAC SexAI 1100 ACCAGGT A^CC[AT]GGT SfiI 2295 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 2256 CCCGGG CCC^GGG TfiI 2700 GAATC G^A[AT]TC Tth111I 777 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2256 CCCGGG C^CCGGG XmaI 2256 CCCGGG C^CCGGG XmnI 2700 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccI 1198 GTCGAC GT^[AC][GT]AC 1639 GTCTAC AcyI 1285 GGCGCC G[GA]^CG[CT]C 2153 GGCGCC AfaI 1863 GTAC GT^AC 2203 GTAC AocI 475 CCTGAGG CC^T[ACGT]AGG 2290 CCTGAGG ApaI 61 GGGCCC GGGCC^C 214 GGGCCC AvaI 1693 CTCGGG C^[CT]CG[GA]G 2256 CCCGGG AviII 1541 TGCGCA TGC^GCA 1568 TGCGCA BalI 1112 TGGCCA TGG^CCA 2303 TGGCCA BamHI 343 GGATCC G^GATCC 821 GGATCC BbeI 1285 GGCGCC GGCGC^C 2153 GGCGCC BbrPI 1393 CACGTG CAC^GTG 1669 CACGTG BbsI 646 GTCTTC GAAGAC(2/6) 1381 GTCTTC BbvCI 800 CCTCAGC CCTCAGC(-5/-2) 1173 CCTCAGC BpuAI 646 GTCTTC GAAGAC(2/6) 1381 GTCTTC BsaAI 1393 CACGTG [CT]AC^GT[GA] 1669 CACGTG BsaHI 1285 GGCGCC G[GA]^CG[CT]C 2153 GGCGCC BsoBI 1693 CTCGGG C^[CT]CG[GA]G 2256 CCCGGG BssSI 1489 CACGAG CACGAG(-5/-1) 1940 CTCGTG BstEII 1698 GGTCACC G^GT[ACGT]ACC 2724 GGTGACC BstPI 1698 GGTCACC G^GT[ACGT]ACC 2724 GGTGACC Bsu36I 475 CCTGAGG CC^T[ACGT]AGG 2290 CCTGAGG Eco81I 475 CCTGAGG CC^T[ACGT]AGG 2290 CCTGAGG EcoO65I 1698 GGTCACC G^GT[ACGT]ACC 2724 GGTGACC FspI 1541 TGCGCA TGC^GCA 1568 TGCGCA Hin1I 1285 GGCGCC G[GA]^CG[CT]C 2153 GGCGCC KasI 1285 GGCGCC G^GCGCC 2153 GGCGCC MluNI 1112 TGGCCA TGG^CCA 2303 TGGCCA MscI 1112 TGGCCA TGG^CCA 2303 TGGCCA NaeI 865 GCCGGC GCC^GGC 1131 GCCGGC NarI 1285 GGCGCC GG^CGCC 2153 GGCGCC NgoMIV 865 GCCGGC G^CCGGC 1131 GCCGGC PflMI 567 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2451 CCAGAAGCTGG PmaCI 1393 CACGTG CAC^GTG 1669 CACGTG PmlI 1393 CACGTG CAC^GTG 1669 CACGTG RsaI 1863 GTAC GT^AC 2203 GTAC SacI 972 GAGCTC GAGCT^C 1888 GAGCTC SfoI 1285 GGCGCC GGC^GCC 2153 GGCGCC Tsp509I 662 AATT ^AATT 2406 AATT Van91I 567 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2451 CCAGAAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI AvaII BanI BanII BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII