Restriction map for
pF1KE3356
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII AclI AcyI AgeI AhdI
AocI ApaI AscI AseI AsnI
Asp700I AspEI AspI AvrII BalI
BbeI BbrPI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsiWI BsmBI BspDI BspHI
BsrBI BsrDI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII DrdI Eam1105I
Eco81I EcoO65I EcoRI EcoRV FbaI
FseI Hin1I HpaI KasI KspI
MamI MluI MluNI MscI NarI
NcoI NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 733 GGTACC G^GTACC
AccI 2252 GTCGAC GT^[AC][GT]AC
AccIII 1950 TCCGGA T^CCGGA
AflII 1726 CTTAAG C^TTAAG
Aor51HI 1765 AGCGCT AGC^GCT
Asp718I 733 GGTACC G^GTACC
AviII 1653 TGCGCA TGC^GCA
BamHI 1089 GGATCC G^GATCC
BbvCI 862 CCTCAGC CCTCAGC(-5/-2)
BciVI 2880 GTATCC GTATCC(6/5)
BfrI 1726 CTTAAG C^TTAAG
BseAI 1950 TCCGGA T^CCGGA
BsiEI 620 CGGCCG CG[GA][CT]^CG
Bsp1407I 47 TGTACA T^GTACA
BspEI 1950 TCCGGA T^CCGGA
BspMI 1713 ACCTGC ACCTGC(4/8)
BsrGI 47 TGTACA T^GTACA
BssHII 743 GCGCGC G^CGCGC
BstXI 1226 CCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2058 TTTAAA TTT^AAA
DraII 2402 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 620 CGGCCG [CT]^GGCC[GA]
EagI 620 CGGCCG C^GGCCG
EclXI 620 CGGCCG C^GGCCG
Eco47III 1765 AGCGCT AGC^GCT
Eco52I 620 CGGCCG C^GGCCG
EcoNI 2389 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2402 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 855 ATGCAT ATGCA^T
FspI 1653 TGCGCA TGC^GCA
HgaI 1084 GACGC GACGC(5/10)
HindIII 1145 AAGCTT A^AGCTT
KpnI 733 GGTACC GGTAC^C
MfeI 2085 CAATTG C^AATTG
MroI 1950 TCCGGA T^CCGGA
MunI 2085 CAATTG C^AATTG
NaeI 1207 GCCGGC GCC^GGC
NdeI 2836 CATATG CA^TATG
NgoMIV 1207 GCCGGC G^CCGGC
NsiI 855 ATGCAT ATGCA^T
PaeR7I 780 CTCGAG C^TCGAG
PshAI 2756 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2919 GAGCTC GAGCT^C
SalI 2252 GTCGAC G^TCGAC
SapI 2077 GCTCTTC GCTCTTC(1/4)
SmaI 2794 CCCGGG CCC^GGG
SspBI 47 TGTACA T^GTACA
SspI 1459 AATATT AAT^ATT
XhoI 780 CTCGAG C^TCGAG
XmaCI 2794 CCCGGG C^CCGGG
XmaI 2794 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 613 GTGCAC G^TGCAC
709 GTGCAC
AlwNI 2185 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2890 CAGGCGCTG
ApaLI 613 GTGCAC G^TGCAC
709 GTGCAC
BfaI 2306 CTAG C^TAG
2429 CTAG
BsaI 813 GAGACC GGTCTC(1/5)
2005 GAGACC
BsgI 2546 CTGCAC GTGCAG(16/14)
1022 GTGCAG
BsmI 770 GAATGC GAATGC(1/-1)
3111 GAATGC
BspLU11I 246 ACATGT A^CATGT
476 ACATGT
BsrFI 263 ACCGGC [GA]^CCGG[CT]
1207 GCCGGC
BstAPI 200 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2889 GCAGGCGCTGC
Cfr10I 263 ACCGGC [GA]^CCGG[CT]
1207 GCCGGC
FauI 2268 CCCGC CCCGC(4/6)
2653 CCCGC
HincII 2252 GTCGAC GT[CT]^[GA]AC
2785 GTTGAC
HindII 2252 GTCGAC GT[CT]^[GA]AC
2785 GTTGAC
MaeI 2306 CTAG C^TAG
2429 CTAG
PflMI 1161 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2184 CCAGAGACTGG
StuI 1026 AGGCCT AGG^CCT
3043 AGGCCT
Tsp45I 13 GTGAC ^GT[GC]AC
2045 GTCAC
Van91I 1161 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2184 CCAGAGACTGG
XcmI 1223 CCACCATGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2828 CCAAGTACCATATGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcgI BcnI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XhoII