Restriction map for
pF1KE2549
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# NO CUT ENZYMES ( 71 enzymes) #
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Acc65I AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BciVI BclI BfrI
BlnI BsiWI BspDI BsrBI Bst1107I
BstBI BstXI BstZ17I ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoT22I FbaI FspI HindIII HpaI
KpnI MfeI MluI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SexAI SfuI SnaBI SpeI
SspI SwaI Tru9I Tsp509I XbaI
XhoI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 2911 GTCTAC GT^[AC][GT]AC
AccIII 1229 TCCGGA T^CCGGA
AflIII 351 ACATGT A^C[GA][CT]GT
Asp700I 1500 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 385 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1656 GGCGCC GGCGC^C
BfaI 829 CTAG C^TAG
BlpI 3667 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3667 GCTGAGC GC^T[ACGT]AGC
BsaBI 966 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1229 TCCGGA T^CCGGA
Bsp1407I 4271 TGTACA T^GTACA
BspEI 1229 TCCGGA T^CCGGA
BspLU11I 351 ACATGT A^CATGT
BsrGI 4271 TGTACA T^GTACA
BssHII 1646 GCGCGC G^CGCGC
BstEII 516 GGTGACC G^GT[ACGT]ACC
BstPI 516 GGTGACC G^GT[ACGT]ACC
CelII 3667 GCTGAGC GC^T[ACGT]AGC
EcoO65I 516 GGTGACC G^GT[ACGT]ACC
EcoRV 1222 GATATC GAT^ATC
FseI 3050 GGCCGGCC GGCCGG^CC
HincII 3463 GTTGAC GT[CT]^[GA]AC
HindII 3463 GTTGAC GT[CT]^[GA]AC
KasI 1656 GGCGCC G^GCGCC
MaeI 829 CTAG C^TAG
MamI 966 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1229 TCCGGA T^CCGGA
NaeI 3051 GCCGGC GCC^GGC
NarI 1656 GGCGCC GG^CGCC
NgoMIV 3051 GCCGGC G^CCGGC
NotI 3847 GCGGCCGC GC^GGCCGC
PflFI 385 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 703 GAGCTC GAGCT^C
SbfI 2651 CCTGCAGG CCTGCA^GG
SfiI 2850 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1656 GGCGCC GGC^GCC
SgrAI 633 CACCGGCG C[GA]^CCGG[CT]G
SmlI 3325 CTCAAG C^T[CT][GA]AG
SphI 2276 GCATGC GCATG^C
Sse8387I 2651 CCTGCAGG CCTGCA^GG
SspBI 4271 TGTACA T^GTACA
Tth111I 385 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1500 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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ApaI 2761 GGGCCC GGGCC^C
4154 GGGCCC
BamHI 1626 GGATCC G^GATCC
4211 GGATCC
BbrPI 1678 CACGTG CAC^GTG
3715 CACGTG
BcgI 1080 CGACGTCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1190 GCAGGATTGTCG
BsaI 1174 GAGACC GGTCTC(1/5)
87 GGTCTC
BsmBI 3793 GAGACG CGTCTC(1/5)
4321 GAGACG
BsmI 211 GAATGC GAATGC(1/-1)
3720 GAATGC
BspHI 470 TCATGA T^CATGA
641 TCATGA
BsrDI 1086 CATTGC GCAATG(2/0)
1398 CATTGC
BssSI 1345 CACGAG CACGAG(-5/-1)
856 CTCGTG
BstAPI 1302 GCAGGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1608 GCAGAAGGTGC
EagI 3225 CGGCCG C^GGCCG
3848 CGGCCG
EclXI 3225 CGGCCG C^GGCCG
3848 CGGCCG
Eco52I 3225 CGGCCG C^GGCCG
3848 CGGCCG
NspI 351 ACATGT [GA]CATG^[CT]
2276 GCATGC
PflMI 2219 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4415 CCATCCGATGG
PmaCI 1678 CACGTG CAC^GTG
3715 CACGTG
PmlI 1678 CACGTG CAC^GTG
3715 CACGTG
RcaI 470 TCATGA T^CATGA
641 TCATGA
Van91I 2219 CCAAGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4415 CCATCCGATGG
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# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
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AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StuI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII XmaCI XmaI