Restriction map for
pF1KE2582
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BbeI
BbrPI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoRV EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
MamI MfeI MluI MroI MunI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StuI SwaI
Tth111I XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 1088 GGTACC G^GTACC
AccII 2686 CGCG CG^CG
AocI 237 CCTGAGG CC^T[ACGT]AGG
ApaI 877 GGGCCC GGGCC^C
Asp718I 1088 GGTACC G^GTACC
BamHI 2027 GGATCC G^GATCC
BcgI 2319 GCAGCGATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2035 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 1547 GAGACC GGTCTC(1/5)
BsaWI 527 TCCGGT [AT]^CCGG[AT]
BsiEI 2688 CGGCCG CG[GA][CT]^CG
BsmI 2106 GAATGC GAATGC(1/-1)
BspHI 1533 TCATGA T^CATGA
BspMI 898 GCAGGT ACCTGC(4/8)
BsrFI 2690 GCCGGC [GA]^CCGG[CT]
BssSI 1027 CACGAG CACGAG(-5/-1)
BstAPI 2113 GCAGCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1003 GGTGACC G^GT[ACGT]ACC
BstPI 1003 GGTGACC G^GT[ACGT]ACC
BstUI 2686 CGCG CG^CG
Bsu36I 237 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2690 GCCGGC [GA]^CCGG[CT]
DraIII 493 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2688 CGGCCG C^GGCCG
EclXI 2688 CGGCCG C^GGCCG
Eco52I 2688 CGGCCG C^GGCCG
Eco81I 237 CCTGAGG CC^T[ACGT]AGG
EcoNI 1740 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1003 GGTGACC G^GT[ACGT]ACC
EcoRI 1654 GAATTC G^AATTC
HaeII 744 GGCGCT [GA]GCGC^[CT]
KpnI 1088 GGTACC GGTAC^C
KspI 2685 CCGCGG CCGC^GG
MvnI 2686 CGCG CG^CG
NaeI 2690 GCCGGC GCC^GGC
NdeI 1811 CATATG CA^TATG
NgoMIV 2690 GCCGGC G^CCGGC
RcaI 1533 TCATGA T^CATGA
SacII 2685 CCGCGG CCGC^GG
SapI 2652 GCTCTTC GCTCTTC(1/4)
SbfI 895 CCTGCAGG CCTGCA^GG
SexAI 494 ACCAGGT A^CC[AT]GGT
SfiI 2034 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 434 CTCAAG C^T[CT][GA]AG
SphI 2517 GCATGC GCATG^C
Sse8387I 895 CCTGCAGG CCTGCA^GG
Tsp45I 1004 GTGAC ^GT[GC]AC
XbaI 93 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 454 GTCTAC GT^[AC][GT]AC
1464 GTCTAC
AcsI 1433 GAATTT [GA]^AATT[CT]
1654 GAATTC
ApoI 1433 GAATTT [GA]^AATT[CT]
1654 GAATTC
BfaI 94 CTAG C^TAG
1564 CTAG
BlpI 780 GCTCAGC GC^T[ACGT]AGC
2694 GCTGAGC
Bpu1102I 780 GCTCAGC GC^T[ACGT]AGC
2694 GCTGAGC
BstXI 1008 CCAGCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1281 CCAGCAGCTTGG
CelII 780 GCTCAGC GC^T[ACGT]AGC
2694 GCTGAGC
EcoT14I 645 CCATGG C^C[AT][AT]GG
2778 CCATGG
MaeI 94 CTAG C^TAG
1564 CTAG
MaeII 770 ACGT A^CGT
2364 ACGT
MslI 1948 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2512 CATGAGCATG
NcoI 645 CCATGG C^CATGG
2778 CCATGG
PflMI 1026 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1323 CCAGCAAATGG
PleI 100 GACTC GAGTC(4/5)
2350 GACTC
SacI 2181 GAGCTC GAGCT^C
2650 GAGCTC
StyI 645 CCATGG C^C[AT][AT]GG
2778 CCATGG
TaqI 1560 TCGA T^CGA
1733 TCGA
TfiI 791 GAATC G^A[AT]TC
1480 GAATC
TthHB8I 1560 TCGA T^CGA
1733 TCGA
Van91I 1026 CCACGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1323 CCAGCAAATGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp509I TspRI XcmI XhoII