Restriction map for
pF1KE2646
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# NO CUT ENZYMES ( 41 enzymes) #
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AgeI Asp700I AspI AviII AvrII
BbrPI BcgI BlnI BsaBI BsiWI
Bst1107I BstBI BstZ17I DraI DraIII
FseI FspI HpaI MamI MluI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PvuI
SbfI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 5635 GACGTC GACGT^C
AccIII 3943 TCCGGA T^CCGGA
AclI 3388 AACGTT AA^CGTT
AflII 5547 CTTAAG C^TTAAG
Alw44I 3706 GTGCAC G^TGCAC
AocI 4900 CCTGAGG CC^T[ACGT]AGG
ApaLI 3706 GTGCAC G^TGCAC
AscI 436 GGCGCGCC GG^CGCGCC
AseI 4972 ATTAAT AT^TAAT
AsnI 4972 ATTAAT AT^TAAT
BfrI 5547 CTTAAG C^TTAAG
BsaAI 4953 TACGTG [CT]AC^GT[GA]
BseAI 3943 TCCGGA T^CCGGA
BspDI 5629 ATCGAT AT^CGAT
BspEI 3943 TCCGGA T^CCGGA
BspHI 4521 TCATGA T^CATGA
BspMI 5557 GCAGGT ACCTGC(4/8)
Bsu36I 4900 CCTGAGG CC^T[ACGT]AGG
ClaI 5629 ATCGAT AT^CGAT
Eco81I 4900 CCTGAGG CC^T[ACGT]AGG
HindIII 6480 AAGCTT A^AGCTT
MfeI 1676 CAATTG C^AATTG
MroI 3943 TCCGGA T^CCGGA
MunI 1676 CAATTG C^AATTG
NdeI 4123 CATATG CA^TATG
NheI 6606 GCTAGC G^CTAGC
PaeR7I 6049 CTCGAG C^TCGAG
PshBI 4972 ATTAAT AT^TAAT
Psp1406I 3388 AACGTT AA^CGTT
RcaI 4521 TCATGA T^CATGA
SalI 3217 GTCGAC G^TCGAC
SapI 5596 GAAGAGC GCTCTTC(1/4)
ScaI 5054 AGTACT AGT^ACT
StuI 5907 AGGCCT AGG^CCT
XhoI 6049 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 3217 GTCGAC GT^[AC][GT]AC
6574 GTAGAC
BamHI 1554 GGATCC G^GATCC
3559 GGATCC
BclI 756 TGATCA T^GATCA
5133 TGATCA
BglII 1954 AGATCT A^GATCT
2037 AGATCT
BsaWI 3943 TCCGGA [AT]^CCGG[AT]
5738 ACCGGA
BsmBI 178 GAGACG CGTCTC(1/5)
5777 GAGACG
BsmI 1715 GAATGC GAATGC(1/-1)
2693 GAATGC
BssHII 437 GCGCGC G^CGCGC
1331 GCGCGC
BstAPI 1881 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2095 GCAGTCAGTGC
BstEII 3622 GGTGACC G^GT[ACGT]ACC
4773 GGTCACC
BstPI 3622 GGTGACC G^GT[ACGT]ACC
4773 GGTCACC
CpoI 1518 CGGACCG CG^G[AT]CCG
4241 CGGACCG
DrdI 3579 GACCCCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4623 GACGCCACCGTC
EcoO65I 3622 GGTGACC G^GT[ACGT]ACC
4773 GGTCACC
EcoRI 5087 GAATTC G^AATTC
6640 GAATTC
FbaI 756 TGATCA T^GATCA
5133 TGATCA
PpuMI 3836 AGGACCC [GA]G^G[AT]CC[CT]
6210 GGGACCC
RsrII 1518 CGGACCG CG^G[AT]CCG
4241 CGGACCG
SspI 4975 AATATT AAT^ATT
5848 AATATT
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# 3 OR MORE CUT ENZYMES ( 163 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI Aor51HI ApaI ApoI Asp718I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoRII EcoRV EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NotI NsiI
NspI PflMI PleI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI SphI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI