################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BciVI BclI BfaI BfrI BlnI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI MaeI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SapI SbfI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AhdI 811 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1084 GTGCAC G^TGCAC AocI 532 CCTGAGG CC^T[ACGT]AGG ApaLI 1084 GTGCAC G^TGCAC AspEI 811 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1567 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 355 CACGTG CAC^GTG BcgI 1504 CGAGGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 11 AGATCT A^GATCT BlpI 444 GCTCAGC GC^T[ACGT]AGC Bpu1102I 444 GCTCAGC GC^T[ACGT]AGC BsaAI 355 CACGTG [CT]AC^GT[GA] BsaI 1354 GAGACC GGTCTC(1/5) BsiEI 712 CGGCCG CG[GA][CT]^CG BsmI 174 GCATTC GAATGC(1/-1) BsrDI 746 GCAATG GCAATG(2/0) BsrFI 590 ACCGGC [GA]^CCGG[CT] BstBI 823 TTCGAA TT^CGAA Bsu36I 532 CCTGAGG CC^T[ACGT]AGG CelII 444 GCTCAGC GC^T[ACGT]AGC CfoI 1050 GCGC GCG^C Cfr10I 590 ACCGGC [GA]^CCGG[CT] DraIII 296 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 712 CGGCCG C^GGCCG Eam1105I 811 GACGAGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 712 CGGCCG C^GGCCG Eco52I 712 CGGCCG C^GGCCG Eco81I 532 CCTGAGG CC^T[ACGT]AGG EcoNI 309 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 1211 CCCGC CCCGC(4/6) HgaI 1047 GACGC GACGC(5/10) HhaI 1050 GCGC GCG^C HinP1I 1050 GCGC G^CGC KspI 715 CCGCGG CCGC^GG NotI 711 GCGGCCGC GC^GGCCGC NspV 823 TTCGAA TT^CGAA PflFI 1567 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 588 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 355 CACGTG CAC^GTG PmlI 355 CACGTG CAC^GTG SacI 1456 GAGCTC GAGCT^C SacII 715 CCGCGG CCGC^GG ScaI 1178 AGTACT AGT^ACT SexAI 1055 ACCTGGT A^CC[AT]GGT SfcI 1014 CTACAG C^T[GA][CT]AG SfuI 823 TTCGAA TT^CGAA SmaI 505 CCCGGG CCC^GGG TfiI 112 GAATC G^A[AT]TC Tth111I 1567 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 588 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 505 CCCGGG C^CCGGG XmaI 505 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 816 GACGTC GACGT^C 1570 GACGTC AcyI 816 GACGTC G[GA]^CG[CT]C 1570 GACGTC AflIII 354 ACACGT A^C[GA][CT]GT 686 ACACGT AlwNI 265 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1139 CAGATCCTG ApaI 55 GGGCCC GGGCC^C 528 GGGCCC AspHI 1084 GTGCAC G[AT]GC[AT]^C 1456 GAGCTC BalI 377 TGGCCA TGG^CCA 1271 TGGCCA BbsI 723 GAAGAC GAAGAC(2/6) 661 GTCTTC BglI 394 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1598 GCCTTCGGGGC BpuAI 723 GAAGAC GAAGAC(2/6) 661 GTCTTC BsaHI 816 GACGTC G[GA]^CG[CT]C 1570 GACGTC BseRI 33 CTCCTC GAGGAG(10/8) 121 GAGGAG BsiHKAI 1084 GTGCAC G[AT]GC[AT]^C 1456 GAGCTC BsmBI 814 GAGACG CGTCTC(1/5) 1045 GAGACG BstAPI 295 GCACTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1138 GCAGATCCTGC EarI 118 GAAGAG CTCTTC(1/4) 130 GAAGAG Hin1I 816 GACGTC G[GA]^CG[CT]C 1570 GACGTC Ksp632I 118 GAAGAG CTCTTC(1/4) 130 GAAGAG MluNI 377 TGGCCA TGG^CCA 1271 TGGCCA MscI 377 TGGCCA TGG^CCA 1271 TGGCCA MseI 557 TTAA T^TAA 1484 TTAA NspI 602 ACATGC [GA]CATG^[CT] 1088 ACATGC PleI 988 GACTC GAGTC(4/5) 1009 GACTC PpuMI 75 GGGTCCC [GA]G^G[AT]CC[CT] 630 GGGACCC PvuII 207 CAGCTG CAG^CTG 880 CAGCTG StuI 536 AGGCCT AGG^CCT 1373 AGGCCT Tru9I 557 TTAA T^TAA 1484 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaI AvaII BanI BanII BbvCI BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII