Restriction map for
pF1KE2617
##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AclI AflIII AgeI Alw44I
ApaLI AscI AseI AsnI Asp718I
BamHI BbrPI BcgI BciVI BsiWI
BspLU11I Bst1107I BstZ17I CpoI EcoRV
FseI HindIII HpaI KpnI KspI
MluI NdeI NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SnaBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 22 enzymes) #
###################################
Aor51HI 199 AGCGCT AGC^GCT
AviII 5108 TGCGCA TGC^GCA
BclI 4541 TGATCA T^GATCA
BspDI 1339 ATCGAT AT^CGAT
BspHI 3527 TCATGA T^CATGA
BstAPI 986 GCACGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3400 TTCGAA TT^CGAA
ClaI 1339 ATCGAT AT^CGAT
Eco47III 199 AGCGCT AGC^GCT
EcoT22I 3986 ATGCAT ATGCA^T
FbaI 4541 TGATCA T^GATCA
FspI 5108 TGCGCA TGC^GCA
MfeI 408 CAATTG C^AATTG
MunI 408 CAATTG C^AATTG
NsiI 3986 ATGCAT ATGCA^T
NspV 3400 TTCGAA TT^CGAA
PpuMI 2759 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 3527 TCATGA T^CATGA
SalI 6506 GTCGAC G^TCGAC
SfuI 3400 TTCGAA TT^CGAA
SgrAI 1519 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 2881 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 3949 GACGTC GACGT^C
5634 GACGTC
AccI 6506 GTCGAC GT^[AC][GT]AC
6541 GTCTAC
AcsI 774 GAATTC [GA]^AATT[CT]
2124 GAATTC
AflII 2599 CTTAAG C^TTAAG
2908 CTTAAG
AhdI 2434 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5359 GACACTGAGTC
AocI 3075 CCTGAGG CC^T[ACGT]AGG
4851 CCTGAGG
ApoI 774 GAATTC [GA]^AATT[CT]
2124 GAATTC
AspEI 2434 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5359 GACACTGAGTC
AvrII 2856 CCTAGG C^CTAGG
5989 CCTAGG
BfrI 2599 CTTAAG C^TTAAG
2908 CTTAAG
BlnI 2856 CCTAGG C^CTAGG
5989 CCTAGG
BsaAI 3282 CACGTA [CT]AC^GT[GA]
3700 TACGTG
BsaBI 3483 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3531 GATGAACATC
BsmI 90 GAATGC GAATGC(1/-1)
3262 GAATGC
BsrBI 3426 CCGCTC CCGCTC(-3/-3)
5808 CCGCTC
BssHII 6495 GCGCGC G^CGCGC
6497 GCGCGC
BssSI 1890 CACGAG CACGAG(-5/-1)
5377 CACGAG
Bsu36I 3075 CCTGAGG CC^T[ACGT]AGG
4851 CCTGAGG
DraI 525 TTTAAA TTT^AAA
4633 TTTAAA
EagI 5283 CGGCCG C^GGCCG
6374 CGGCCG
Eam1105I 2434 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5359 GACACTGAGTC
EclXI 5283 CGGCCG C^GGCCG
6374 CGGCCG
Eco52I 5283 CGGCCG C^GGCCG
6374 CGGCCG
Eco81I 3075 CCTGAGG CC^T[ACGT]AGG
4851 CCTGAGG
EcoRI 774 GAATTC G^AATTC
2124 GAATTC
MamI 3483 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3531 GATGAACATC
NaeI 2379 GCCGGC GCC^GGC
5488 GCCGGC
NgoMIV 2379 GCCGGC G^CCGGC
5488 GCCGGC
PflMI 4617 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6324 CCAGGAGCTGG
PvuII 3327 CAGCTG CAG^CTG
4058 CAGCTG
SacI 862 GAGCTC GAGCT^C
6523 GAGCTC
SbfI 1737 CCTGCAGG CCTGCA^GG
4963 CCTGCAGG
ScaI 3063 AGTACT AGT^ACT
5021 AGTACT
SexAI 1916 ACCTGGT A^CC[AT]GGT
4949 ACCAGGT
SfiI 1470 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1683 GGCCCTGCTGGCC
SphI 1394 GCATGC GCATG^C
4418 GCATGC
Sse8387I 1737 CCTGCAGG CCTGCA^GG
4963 CCTGCAGG
Van91I 4617 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6324 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI ApaI Asp700I
AspHI AspI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspEI BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MroI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmaCI XmaI
XmnI