################################## # NO CUT ENZYMES ( 47 enzymes) # ################################## Acc65I AclI AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I BamHI BbrPI BcgI BciVI BsiWI BspLU11I Bst1107I BstZ17I CpoI EcoRV FseI HindIII HpaI KpnI KspI MluI NdeI NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SnaBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 22 enzymes) # ################################### Aor51HI 199 AGCGCT AGC^GCT AviII 5108 TGCGCA TGC^GCA BclI 4541 TGATCA T^GATCA BspDI 1339 ATCGAT AT^CGAT BspHI 3527 TCATGA T^CATGA BstAPI 986 GCACGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3400 TTCGAA TT^CGAA ClaI 1339 ATCGAT AT^CGAT Eco47III 199 AGCGCT AGC^GCT EcoT22I 3986 ATGCAT ATGCA^T FbaI 4541 TGATCA T^GATCA FspI 5108 TGCGCA TGC^GCA MfeI 408 CAATTG C^AATTG MunI 408 CAATTG C^AATTG NsiI 3986 ATGCAT ATGCA^T NspV 3400 TTCGAA TT^CGAA PpuMI 2759 AGGACCC [GA]G^G[AT]CC[CT] RcaI 3527 TCATGA T^CATGA SalI 6506 GTCGAC G^TCGAC SfuI 3400 TTCGAA TT^CGAA SgrAI 1519 CGCCGGTG C[GA]^CCGG[CT]G SpeI 2881 ACTAGT A^CTAGT ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AatII 3949 GACGTC GACGT^C 5634 GACGTC AccI 6506 GTCGAC GT^[AC][GT]AC 6541 GTCTAC AcsI 774 GAATTC [GA]^AATT[CT] 2124 GAATTC AflII 2599 CTTAAG C^TTAAG 2908 CTTAAG AhdI 2434 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5359 GACACTGAGTC AocI 3075 CCTGAGG CC^T[ACGT]AGG 4851 CCTGAGG ApoI 774 GAATTC [GA]^AATT[CT] 2124 GAATTC AspEI 2434 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5359 GACACTGAGTC AvrII 2856 CCTAGG C^CTAGG 5989 CCTAGG BfrI 2599 CTTAAG C^TTAAG 2908 CTTAAG BlnI 2856 CCTAGG C^CTAGG 5989 CCTAGG BsaAI 3282 CACGTA [CT]AC^GT[GA] 3700 TACGTG BsaBI 3483 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3531 GATGAACATC BsmI 90 GAATGC GAATGC(1/-1) 3262 GAATGC BsrBI 3426 CCGCTC CCGCTC(-3/-3) 5808 CCGCTC BssHII 6495 GCGCGC G^CGCGC 6497 GCGCGC BssSI 1890 CACGAG CACGAG(-5/-1) 5377 CACGAG Bsu36I 3075 CCTGAGG CC^T[ACGT]AGG 4851 CCTGAGG DraI 525 TTTAAA TTT^AAA 4633 TTTAAA EagI 5283 CGGCCG C^GGCCG 6374 CGGCCG Eam1105I 2434 GACGGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5359 GACACTGAGTC EclXI 5283 CGGCCG C^GGCCG 6374 CGGCCG Eco52I 5283 CGGCCG C^GGCCG 6374 CGGCCG Eco81I 3075 CCTGAGG CC^T[ACGT]AGG 4851 CCTGAGG EcoRI 774 GAATTC G^AATTC 2124 GAATTC MamI 3483 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3531 GATGAACATC NaeI 2379 GCCGGC GCC^GGC 5488 GCCGGC NgoMIV 2379 GCCGGC G^CCGGC 5488 GCCGGC PflMI 4617 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6324 CCAGGAGCTGG PvuII 3327 CAGCTG CAG^CTG 4058 CAGCTG SacI 862 GAGCTC GAGCT^C 6523 GAGCTC SbfI 1737 CCTGCAGG CCTGCA^GG 4963 CCTGCAGG ScaI 3063 AGTACT AGT^ACT 5021 AGTACT SexAI 1916 ACCTGGT A^CC[AT]GGT 4949 ACCAGGT SfiI 1470 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1683 GGCCCTGCTGGCC SphI 1394 GCATGC GCATG^C 4418 GCATGC Sse8387I 1737 CCTGCAGG CCTGCA^GG 4963 CCTGCAGG Van91I 4617 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 6324 CCAGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 151 enzymes) # ######################################### AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI ApaI Asp700I AspHI AspI AvaI AvaII BalI BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrDI BsrFI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PleI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII XmaCI XmaI XmnI