Restriction map for
pF1KB3277
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI Alw44I ApaLI
AscI Asp700I AspI AvrII BamHI
BbeI BcgI BfrI BglI BglII
BlnI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoNI EcoRI EcoT22I FseI Hin1I
HincII HindII HpaI KasI KspI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI PacI PflFI PinAI PmeI
PpuMI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 82 enzymes) #
###################################
Acc65I 666 GGTACC G^GTACC
AhdI 680 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 236 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1036 CCTAAGG CC^T[ACGT]AGG
Aor51HI 741 AGCGCT AGC^GCT
ApaI 696 GGGCCC GGGCC^C
AseI 967 ATTAAT AT^TAAT
AsnI 967 ATTAAT AT^TAAT
Asp718I 666 GGTACC G^GTACC
AspEI 680 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 119 TGCGCA TGC^GCA
BalI 248 TGGCCA TGG^CCA
BanII 696 GGGCCC G[GA]GC[CT]^C
BbrPI 1567 CACGTG CAC^GTG
BbsI 636 GAAGAC GAAGAC(2/6)
BbvCI 1436 CCTCAGC CCTCAGC(-5/-2)
BciVI 960 GGATAC GTATCC(6/5)
BclI 1335 TGATCA T^GATCA
BlpI 884 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 884 GCTTAGC GC^T[ACGT]AGC
BpuAI 636 GAAGAC GAAGAC(2/6)
BsaBI 780 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1612 GAGACC GGTCTC(1/5)
BsmBI 1607 GAGACG CGTCTC(1/5)
BsmI 620 GAATGC GAATGC(1/-1)
BsrDI 935 CATTGC GCAATG(2/0)
BsrFI 26 GCCGGC [GA]^CCGG[CT]
BstBI 259 TTCGAA TT^CGAA
BstEII 795 GGTCACC G^GT[ACGT]ACC
BstPI 795 GGTCACC G^GT[ACGT]ACC
BstXI 1455 CCATCGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 865 AGATCC [GA]^GATC[CT]
Bsu36I 1036 CCTAAGG CC^T[ACGT]AGG
CelII 884 GCTTAGC GC^T[ACGT]AGC
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
DraI 756 TTTAAA TTT^AAA
DraII 696 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1586 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1617 CCACGG C^C[GA][CT]GG
EaeI 248 TGGCCA [CT]^GGCC[GA]
Eam1105I 680 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1428 CTCTTC CTCTTC(1/4)
Eco47III 741 AGCGCT AGC^GCT
Eco81I 1036 CCTAAGG CC^T[ACGT]AGG
EcoO109I 696 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 795 GGTCACC G^GT[ACGT]ACC
FauI 11 GCGGG CCCGC(4/6)
FbaI 1335 TGATCA T^GATCA
FspI 119 TGCGCA TGC^GCA
HaeII 741 AGCGCT [GA]GCGC^[CT]
HgaI 955 GACGC GACGC(5/10)
HindIII 882 AAGCTT A^AGCTT
KpnI 666 GGTACC GGTAC^C
Ksp632I 1428 CTCTTC CTCTTC(1/4)
MamI 780 GATATCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 865 AGATCC [GA]^GATC[CT]
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
NaeI 26 GCCGGC GCC^GGC
NgoMIV 26 GCCGGC G^CCGGC
NspI 279 GCATGT [GA]CATG^[CT]
NspV 259 TTCGAA TT^CGAA
PaeR7I 1064 CTCGAG C^TCGAG
PflMI 235 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1567 CACGTG CAC^GTG
PmlI 1567 CACGTG CAC^GTG
PshAI 195 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 967 ATTAAT AT^TAAT
PstI 385 CTGCAG CTGCA^G
PvuII 1109 CAGCTG CAG^CTG
SapI 1427 GCTCTTC GCTCTTC(1/4)
SfuI 259 TTCGAA TT^CGAA
SgrAI 25 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1661 CCCGGG CCC^GGG
SnaBI 1534 TACGTA TAC^GTA
StuI 1208 AGGCCT AGG^CCT
Van91I 235 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 674 CCATGCGACCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1064 CTCGAG C^TCGAG
XhoII 865 AGATCC [GA]^GATC[CT]
XmaCI 1661 CCCGGG C^CCGGG
XmaI 1661 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AccII 957 CGCG CG^CG
1474 CGCG
AspHI 643 GAGCAC G[AT]GC[AT]^C
1186 GAGCAC
AvaI 1064 CTCGAG C^[CT]CG[GA]G
1661 CCCGGG
BanI 666 GGTACC G^G[CT][GA]CC
1652 GGCACC
BsaAI 1534 TACGTA [CT]AC^GT[GA]
1567 CACGTG
BsiHKAI 643 GAGCAC G[AT]GC[AT]^C
1186 GAGCAC
BsoBI 1064 CTCGAG C^[CT]CG[GA]G
1661 CCCGGG
BstUI 957 CGCG CG^CG
1474 CGCG
EcoRV 780 GATATC GAT^ATC
1758 GATATC
EcoT14I 998 CCTTGG C^C[AT][AT]GG
1724 CCAAGG
MspA1I 800 CCGCTG C[AC]G^C[GT]G
1109 CAGCTG
MvnI 957 CGCG CG^CG
1474 CGCG
StyI 998 CCTTGG C^C[AT][AT]GG
1724 CCAAGG
TfiI 82 GATTC G^A[AT]TC
347 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I