################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BanII BbeI BcgI BciVI BfrI BglI BglII BlnI BsaHI BsiEI BsiWI BsmBI BsmI BsoBI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HaeII HgaI Hin1I HindIII KasI KpnI KspI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 1039 GTAGAC GT^[AC][GT]AC AccIII 999 TCCGGA T^CCGGA AcsI 1937 AAATTC [GA]^AATT[CT] ApoI 1937 AAATTC [GA]^AATT[CT] AspHI 675 GAGCAC G[AT]GC[AT]^C BamHI 269 GGATCC G^GATCC BbrPI 103 CACGTG CAC^GTG BcnI 266 CCCGG CC^[GC]GG BlpI 48 GCTGAGC GC^T[ACGT]AGC BmyI 675 GAGCAC G[AGT]GC[ACT]^C Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC BsaAI 103 CACGTG [CT]AC^GT[GA] BsaBI 1859 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 999 TCCGGA [AT]^CCGG[AT] BseAI 999 TCCGGA T^CCGGA BseRI 543 CTCCTC GAGGAG(10/8) BsgI 290 GTGCAG GTGCAG(16/14) BsiHKAI 675 GAGCAC G[AT]GC[AT]^C Bsp1286I 675 GAGCAC G[AGT]GC[ACT]^C BspDI 64 ATCGAT AT^CGAT BspEI 999 TCCGGA T^CCGGA BspHI 1116 TCATGA T^CATGA BssSI 40 CTCGTG CACGAG(-5/-1) BstXI 1827 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 48 GCTGAGC GC^T[ACGT]AGC CfoI 58 GCGC GCG^C ClaI 64 ATCGAT AT^CGAT EcoNI 153 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1232 GATATC GAT^ATC FauI 458 CCCGC CCCGC(4/6) HhaI 58 GCGC GCG^C HinP1I 58 GCGC G^CGC HpaI 442 GTTAAC GTT^AAC MamI 1859 GATCTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 999 TCCGGA T^CCGGA MseI 443 TTAA T^TAA MwoI 1282 GCAGCCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 266 CCCGG CC^[GC]GG NcoI 1379 CCATGG C^CATGG NspI 252 GCATGT [GA]CATG^[CT] PmaCI 103 CACGTG CAC^GTG PmlI 103 CACGTG CAC^GTG PshAI 1420 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1116 TCATGA T^CATGA SbfI 227 CCTGCAGG CCTGCA^GG SpeI 679 ACTAGT A^CTAGT Sse8387I 227 CCTGCAGG CCTGCA^GG Tru9I 443 TTAA T^TAA XcmI 1845 CCATACCACTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AspI 1385 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1740 GACTGGGTC BanI 454 GGCACC G^G[CT][GA]CC 1051 GGCACC BbvCI 1286 CCTCAGC CCTCAGC(-5/-2) 1730 CCTCAGC BclI 1952 TGATCA T^GATCA 2048 TGATCA BsrDI 2001 CATTGC GCAATG(2/0) 2060 GCAATG BstYI 269 GGATCC [GA]^GATC[CT] 1858 GGATCT DsaI 934 CCACGG C^C[GA][CT]GG 1379 CCATGG EarI 1126 GAAGAG CTCTTC(1/4) 1631 GAAGAG EcoT14I 1379 CCATGG C^C[AT][AT]GG 1976 CCTTGG FbaI 1952 TGATCA T^GATCA 2048 TGATCA HapII 267 CCGG C^CGG 1000 CCGG HincII 442 GTTAAC GT[CT]^[GA]AC 2008 GTCAAC HindII 442 GTTAAC GT[CT]^[GA]AC 2008 GTCAAC HpaII 267 CCGG C^CGG 1000 CCGG Ksp632I 1126 GAAGAG CTCTTC(1/4) 1631 GAAGAG MflI 269 GGATCC [GA]^GATC[CT] 1858 GGATCT MspI 267 CCGG C^CGG 1000 CCGG PflFI 1385 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1740 GACTGGGTC PvuII 1277 CAGCTG CAG^CTG 2177 CAGCTG StyI 1379 CCATGG C^C[AT][AT]GG 1976 CCTTGG Tth111I 1385 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1740 GACTGGGTC XhoII 269 GGATCC [GA]^GATC[CT] 1858 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BbsI BbvI BfaI BpmI BpuAI BsaI BsaJI BsiYI BslI BsmAI BsmFI Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MvaI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I