Restriction map for
pF1KE3374
##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
Acc65I AccI AclI AflII Aor51HI
AscI AseI AsnI Asp718I AspI
AvrII BciVI BclI BfrI BglII
BlnI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII Eco47III EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI KpnI MfeI MluI MseI
MunI NheI NotI NsiI NspV
PacI PflFI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SbfI
SexAI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2265 GACGTC GACGT^C
AccIII 815 TCCGGA T^CCGGA
AflIII 878 ACGTGT A^C[GA][CT]GT
AgeI 1886 ACCGGT A^CCGGT
AhdI 2310 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2310 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1786 CACGTG CAC^GTG
BbvCI 558 CCTCAGC CCTCAGC(-5/-2)
BfaI 2617 CTAG C^TAG
BlpI 1834 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1834 GCTGAGC GC^T[ACGT]AGC
BsaAI 1786 CACGTG [CT]AC^GT[GA]
BsaBI 2805 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 815 TCCGGA T^CCGGA
BspEI 815 TCCGGA T^CCGGA
CelII 1834 GCTGAGC GC^T[ACGT]AGC
EagI 695 CGGCCG C^GGCCG
Eam1105I 2310 GACGCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 695 CGGCCG C^GGCCG
Eco52I 695 CGGCCG C^GGCCG
HincII 327 GTTGAC GT[CT]^[GA]AC
HindII 327 GTTGAC GT[CT]^[GA]AC
HindIII 2380 AAGCTT A^AGCTT
KspI 503 CCGCGG CCGC^GG
MaeI 2617 CTAG C^TAG
MamI 2805 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 815 TCCGGA T^CCGGA
NcoI 2426 CCATGG C^CATGG
NdeI 848 CATATG CA^TATG
PinAI 1886 ACCGGT A^CCGGT
PmaCI 1786 CACGTG CAC^GTG
PmlI 1786 CACGTG CAC^GTG
SacI 2620 GAGCTC GAGCT^C
SacII 503 CCGCGG CCGC^GG
ScaI 911 AGTACT AGT^ACT
SfiI 592 GGCCCTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 214 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2522 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1011 AAATTT [GA]^AATT[CT]
1984 AAATTC
ApoI 1011 AAATTT [GA]^AATT[CT]
1984 AAATTC
Asp700I 1131 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2967 GAACGACTTC
BalI 1175 TGGCCA TGG^CCA
2066 TGGCCA
BcgI 946 CGAAGAAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2396 GCATCCGCATCG
BsaWI 815 TCCGGA [AT]^CCGG[AT]
1886 ACCGGT
BsmBI 3023 GAGACG CGTCTC(1/5)
3340 GAGACG
BspHI 1442 TCATGA T^CATGA
3218 TCATGA
BssSI 1957 CACGAG CACGAG(-5/-1)
1188 CTCGTG
DrdI 873 GACCAACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1933 GACGCCCACGTC
EcoNI 1361 CCTTCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1548 CCTGAGCCAGG
MluNI 1175 TGGCCA TGG^CCA
2066 TGGCCA
MscI 1175 TGGCCA TGG^CCA
2066 TGGCCA
NaeI 215 GCCGGC GCC^GGC
625 GCCGGC
NgoMIV 215 GCCGGC G^CCGGC
625 GCCGGC
NruI 725 TCGCGA TCG^CGA
2319 TCGCGA
NspI 2522 GCATGC [GA]CATG^[CT]
2686 ACATGC
PaeR7I 1964 CTCGAG C^TCGAG
2242 CTCGAG
PstI 1234 CTGCAG CTGCA^G
2281 CTGCAG
RcaI 1442 TCATGA T^CATGA
3218 TCATGA
SapI 605 GAAGAGC GCTCTTC(1/4)
2941 GAAGAGC
TfiI 1039 GAATC G^A[AT]TC
1136 GATTC
Tsp509I 1012 AATT ^AATT
1985 AATT
XhoI 1964 CTCGAG C^TCGAG
2242 CTCGAG
XmnI 1131 GAAGGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2967 GAACGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII AviII
BamHI BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII