Restriction map for
pF1KE2790
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AviII AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI DdeI
DraI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MaeII MamI MfeI MluI MluNI
MscI MseI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsaI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tru9I Tsp509I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 455 TCCGGA T^CCGGA
AcyI 263 GGCGCC G[GA]^CG[CT]C
AlwI 48 GATCC GGATC(4/5)
AspI 463 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 263 GGCGCC G^G[CT][GA]CC
BanII 103 GGGCTC G[GA]GC[CT]^C
BbeI 263 GGCGCC GGCGC^C
BbsI 375 GAAGAC GAAGAC(2/6)
BfaI 235 CTAG C^TAG
BmyI 103 GGGCTC G[AGT]GC[ACT]^C
BpmI 190 CTGGAG CTGGAG(16/14)
BpuAI 375 GAAGAC GAAGAC(2/6)
BsaHI 263 GGCGCC G[GA]^CG[CT]C
BsaWI 455 TCCGGA [AT]^CCGG[AT]
BseAI 455 TCCGGA T^CCGGA
Bsp1286I 103 GGGCTC G[AGT]GC[ACT]^C
BspEI 455 TCCGGA T^CCGGA
BspHI 359 TCATGA T^CATGA
BspMI 445 ACCTGC ACCTGC(4/8)
BsrI 201 ACTGG ACTGG(1/-1)
BstEII 63 GGTGACC G^GT[ACGT]ACC
BstPI 63 GGTGACC G^GT[ACGT]ACC
BstYI 47 AGATCC [GA]^GATC[CT]
CpoI 457 CGGACCG CG^G[AT]CCG
DpnII 48 GATC ^GATC
DraII 211 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 474 GACCCCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 244 GAAGAG CTCTTC(1/4)
EcoO109I 211 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 63 GGTGACC G^GT[ACGT]ACC
FauI 336 GCGGG CCCGC(4/6)
HaeII 263 GGCGCC [GA]GCGC^[CT]
Hin1I 263 GGCGCC G[GA]^CG[CT]C
KasI 263 GGCGCC G^GCGCC
Ksp632I 244 GAAGAG CTCTTC(1/4)
MaeI 235 CTAG C^TAG
MaeIII 64 GTGAC ^GT[ACGT]AC
MboI 48 GATC ^GATC
MflI 47 AGATCC [GA]^GATC[CT]
MroI 455 TCCGGA T^CCGGA
MslI 417 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 334 CAGCGG C[AC]G^C[GT]G
NarI 263 GGCGCC GG^CGCC
NdeII 48 GATC ^GATC
PflFI 463 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 82 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 481 GAGTC GAGTC(4/5)
PstI 331 CTGCAG CTGCA^G
RcaI 359 TCATGA T^CATGA
RsrII 457 CGGACCG CG^G[AT]CCG
Sau3AI 48 GATC ^GATC
SfcI 331 CTGCAG C^T[GA][CT]AG
SfoI 263 GGCGCC GGC^GCC
TfiI 340 GAATC G^A[AT]TC
Tsp45I 64 GTGAC ^GT[GC]AC
Tth111I 463 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 82 CCAGGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 47 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 54 GGACC G^G[AT]CC
458 GGACC
BsrBI 346 GAGCGG CCGCTC(-3/-3)
403 GAGCGG
BstF5I 150 CATCC GGATG(2/0)
417 CATCC
CfoI 264 GCGC GCG^C
318 GCGC
FokI 150 CATCC GGATG(9/13)
417 CATCC
HhaI 264 GCGC GCG^C
318 GCGC
HinP1I 264 GCGC G^CGC
318 GCGC
HinfI 340 GAATC G^A[ACGT]TC
481 GAGTC
HphI 63 GGTGA GGTGA(8/7)
195 GGTGA
NlaIII 168 CATG CATG^
360 CATG
PaeR7I 502 CTCGAG C^TCGAG
511 CTCGAG
SmlI 502 CTCGAG C^T[CT][GA]AG
511 CTCGAG
TaqI 503 TCGA T^CGA
512 TCGA
TspRI 465 CAGTG CA[GC]TG[ACGT][ACGT]^
497 CACTG
TthHB8I 503 TCGA T^CGA
512 TCGA
XhoI 502 CTCGAG C^TCGAG
511 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BsaJI BseRI BsiYI BslI BsoBI
BssKI BstNI Cac8I Cfr13I EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MboII MnlI MspI MvaI MwoI
NciI NlaIV Sau96I ScrFI SfaNI
TseI