################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI AscI Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspMI BsrBI BsrFI BssHII BstEII BstPI BstUI CelII Cfr10I ClaI CpoI DraII DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I FauI FseI FspI HgaI Hin1I KasI KpnI KspI MluI MroI MvnI NaeI NarI NcoI NgoMIV NlaIV NotI NruI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 561 GTATAC GT^[AC][GT]AC AciI 1418 CCGC CCGC(-3/-1) AflIII 2841 ACATGT A^C[GA][CT]GT AocI 2458 CCTAAGG CC^T[ACGT]AGG Aor51HI 3441 AGCGCT AGC^GCT AspHI 5265 GAGCTC G[AT]GC[AT]^C AspI 428 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1952 CTCGAG C^[CT]CG[GA]G BbvCI 4462 GCTGAGG CCTCAGC(-5/-2) BcnI 1293 CCGGG CC^[GC]GG BsaAI 2975 TACGTA [CT]AC^GT[GA] BsaBI 4401 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 2845 GTGCAG GTGCAG(16/14) BsiEI 2602 CGACCG CG[GA][CT]^CG BsiHKAI 5265 GAGCTC G[AT]GC[AT]^C BsoBI 1952 CTCGAG C^[CT]CG[GA]G Bsp1407I 4620 TGTACA T^GTACA BspLU11I 2841 ACATGT A^CATGT BsrGI 4620 TGTACA T^GTACA Bst1107I 561 GTATAC GTA^TAC BstAPI 3796 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 4217 CCACAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 561 GTATAC GTA^TAC Bsu36I 2458 CCTAAGG CC^T[ACGT]AGG DraIII 3458 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 428 GACAGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3441 AGCGCT AGC^GCT Eco81I 2458 CCTAAGG CC^T[ACGT]AGG EcoT22I 285 ATGCAT ATGCA^T HaeII 3441 AGCGCT [GA]GCGC^[CT] HapII 1293 CCGG C^CGG HindIII 100 AAGCTT A^AGCTT HpaI 3693 GTTAAC GTT^AAC HpaII 1293 CCGG C^CGG MamI 4401 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 1293 CCGG C^CGG NciI 1293 CCGGG CC^[GC]GG NdeI 2365 CATATG CA^TATG NheI 640 GCTAGC G^CTAGC NsiI 285 ATGCAT ATGCA^T PaeR7I 1952 CTCGAG C^TCGAG PflFI 428 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1556 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 5265 GAGCTC GAGCT^C SapI 5267 GCTCTTC GCTCTTC(1/4) SnaBI 2975 TACGTA TAC^GTA SphI 4087 GCATGC GCATG^C SspBI 4620 TGTACA T^GTACA Tth111I 428 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1556 CCATTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1575 TCTAGA T^CTAGA XcmI 776 CCAACTTGAATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1952 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### BalI 4214 TGGCCA TGG^CCA 5162 TGGCCA BanII 1838 GGGCTC G[GA]GC[CT]^C 5265 GAGCTC BmyI 1838 GGGCTC G[AGT]GC[ACT]^C 5265 GAGCTC BpmI 175 CTCCAG CTGGAG(16/14) 3967 CTGGAG BsiYI 1556 CCATTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 4952 CCAAGGCTCGG BslI 1556 CCATTTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 4952 CCAAGGCTCGG BsmFI 490 GGGAC GGGAC(10/14) 1441 GTCCC BsmI 5016 GAATGC GAATGC(1/-1) 509 GCATTC Bsp1286I 1838 GGGCTC G[AGT]GC[ACT]^C 5265 GAGCTC BspHI 114 TCATGA T^CATGA 2882 TCATGA BssSI 1708 CACGAG CACGAG(-5/-1) 1103 CTCGTG CfoI 900 GCGC GCG^C 3442 GCGC EaeI 4214 TGGCCA [CT]^GGCC[GA] 5162 TGGCCA EcoRI 379 GAATTC G^AATTC 3128 GAATTC EcoRV 4608 GATATC GAT^ATC 4696 GATATC HhaI 900 GCGC GCG^C 3442 GCGC HinP1I 900 GCGC G^CGC 3442 GCGC HincII 3693 GTTAAC GT[CT]^[GA]AC 4824 GTCAAC HindII 3693 GTTAAC GT[CT]^[GA]AC 4824 GTCAAC MfeI 62 CAATTG C^AATTG 2828 CAATTG MluNI 4214 TGGCCA TGG^CCA 5162 TGGCCA MscI 4214 TGGCCA TGG^CCA 5162 TGGCCA MspA1I 3367 CAGCTG C[AC]G^C[GT]G 4519 CAGCTG MunI 62 CAATTG C^AATTG 2828 CAATTG PvuII 3367 CAGCTG CAG^CTG 4519 CAGCTG RcaI 114 TCATGA T^CATGA 2882 TCATGA StuI 70 AGGCCT AGG^CCT 3206 AGGCCT SwaI 2379 ATTTAAAT ATTT^AAAT 3650 ATTTAAAT ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AvaII BbsI BbvI BclI BfaI BglII BpuAI BsaJI BseRI BsmAI BsrDI BsrI BssKI BstBI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NspI NspV PleI PshBI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI