################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI Asp700I Asp718I AviII AvrII BamHI BbeI BbvCI BciVI BclI BfrI BglII BlnI BsaBI BsaWI BseAI BsmI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstZ17I ClaI CpoI DraI DraIII Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NruI NsiI PacI PaeR7I PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfoI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 667 GACGTC GACGT^C AcyI 667 GACGTC G[GA]^CG[CT]C AhdI 2239 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 518 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 1783 ATTAAT AT^TAAT AsnI 1783 ATTAAT AT^TAAT AspEI 2239 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1993 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 787 GGTGCC G^G[CT][GA]CC BcgI 2241 CGAGGTGTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 4 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4 GCTGAGC GC^T[ACGT]AGC BsaHI 667 GACGTC G[GA]^CG[CT]C BsaI 581 GAGACC GGTCTC(1/5) BsiEI 540 CGGCCG CG[GA][CT]^CG BsiWI 50 CGTACG C^GTACG Bsp1407I 2129 TGTACA T^GTACA BspMI 584 ACCTGC ACCTGC(4/8) BsrDI 2177 GCAATG GCAATG(2/0) BsrFI 2191 ACCGGC [GA]^CCGG[CT] BsrGI 2129 TGTACA T^GTACA BstBI 22 TTCGAA TT^CGAA BstEII 1902 GGTGACC G^GT[ACGT]ACC BstPI 1902 GGTGACC G^GT[ACGT]ACC CelII 4 GCTGAGC GC^T[ACGT]AGC Cfr10I 2191 ACCGGC [GA]^CCGG[CT] DrdI 661 GACCGCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 540 CGGCCG C^GGCCG Eam1105I 2239 GACGAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1326 CTCTTC CTCTTC(1/4) EclXI 540 CGGCCG C^GGCCG Eco52I 540 CGGCCG C^GGCCG EcoO65I 1902 GGTGACC G^GT[ACGT]ACC EcoT14I 704 CCAAGG C^C[AT][AT]GG HgaI 118 GACGC GACGC(5/10) Hin1I 667 GACGTC G[GA]^CG[CT]C HincII 1888 GTTGAC GT[CT]^[GA]AC HindII 1888 GTTGAC GT[CT]^[GA]AC HindIII 1978 AAGCTT A^AGCTT Ksp632I 1326 CTCTTC CTCTTC(1/4) MseI 1784 TTAA T^TAA MslI 2199 CATCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 2232 GCTAGC G^CTAGC NotI 539 GCGGCCGC GC^GGCCGC NspI 455 GCATGC [GA]CATG^[CT] NspV 22 TTCGAA TT^CGAA PflFI 1993 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1945 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1783 ATTAAT AT^TAAT PvuII 305 CAGCTG CAG^CTG SacI 248 GAGCTC GAGCT^C SfiI 1432 GGCCAGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 22 TTCGAA TT^CGAA SphI 455 GCATGC GCATG^C SspBI 2129 TGTACA T^GTACA StyI 704 CCAAGG C^C[AT][AT]GG Tru9I 1784 TTAA T^TAA Tsp509I 1782 AATT ^AATT Tth111I 1993 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1945 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 1876 GTCTAC GT^[AC][GT]AC 2209 GTAGAC AocI 478 CCTGAGG CC^T[ACGT]AGG 523 CCTGAGG AspHI 201 GAGCAC G[AT]GC[AT]^C 248 GAGCTC BalI 1953 TGGCCA TGG^CCA 2258 TGGCCA BbrPI 361 CACGTG CAC^GTG 910 CACGTG BfaI 577 CTAG C^TAG 2233 CTAG BsaAI 361 CACGTG [CT]AC^GT[GA] 910 CACGTG BsiHKAI 201 GAGCAC G[AT]GC[AT]^C 248 GAGCTC BsmBI 1323 CGTCTC CGTCTC(1/5) 2159 CGTCTC BsrBI 1481 CCGCTC CCGCTC(-3/-3) 1575 CCGCTC BstXI 1619 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2034 CCACGTCCCTGG Bsu36I 478 CCTGAGG CC^T[ACGT]AGG 523 CCTGAGG CfoI 271 GCGC GCG^C 946 GCGC Eco81I 478 CCTGAGG CC^T[ACGT]AGG 523 CCTGAGG HhaI 271 GCGC GCG^C 946 GCGC HinP1I 271 GCGC G^CGC 946 GCGC KspI 1087 CCGCGG CCGC^GG 1411 CCGCGG MaeI 577 CTAG C^TAG 2233 CTAG MluNI 1953 TGGCCA TGG^CCA 2258 TGGCCA MscI 1953 TGGCCA TGG^CCA 2258 TGGCCA PmaCI 361 CACGTG CAC^GTG 910 CACGTG PmlI 361 CACGTG CAC^GTG 910 CACGTG PstI 142 CTGCAG CTGCA^G 1678 CTGCAG SacII 1087 CCGCGG CCGC^GG 1411 CCGCGG SmlI 562 CTCAAG C^T[CT][GA]AG 1375 CTCAAG StuI 707 AGGCCT AGG^CCT 1219 AGGCCT TfiI 133 GAATC G^A[AT]TC 1461 GATTC XcmI 1812 CCAGTCCAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2001 CCAGTCGAAGATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI ApaI AvaI AvaII BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI