Restriction map for
pF1KE2719
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII AccII AccIII AclI AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AviII AvrII
BamHI BbrPI BcgI BlnI BsaAI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I Cfr10I CpoI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I FseI FspI
KspI MluI MroI MvnI NaeI
NgoMIV NotI NruI PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SbfI SfiI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 5837 GGTACC G^GTACC
AcyI 7735 GGCGCC G[GA]^CG[CT]C
AseI 7267 ATTAAT AT^TAAT
AsnI 7267 ATTAAT AT^TAAT
Asp718I 5837 GGTACC G^GTACC
AspHI 4798 GAGCAC G[AT]GC[AT]^C
AspI 3210 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 7735 GGCGCC GGCGC^C
BglI 2524 GCCCGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 747 AGATCT A^GATCT
BsaHI 7735 GGCGCC G[GA]^CG[CT]C
BsiHKAI 4798 GAGCAC G[AT]GC[AT]^C
BspDI 2000 ATCGAT AT^CGAT
BstAPI 2455 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1376 TTCGAA TT^CGAA
CfoI 7736 GCGC GCG^C
ClaI 2000 ATCGAT AT^CGAT
DraIII 1361 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 1648 ATGCAT ATGCA^T
HaeII 7735 GGCGCC [GA]GCGC^[CT]
HhaI 7736 GCGC GCG^C
Hin1I 7735 GGCGCC G[GA]^CG[CT]C
HinP1I 7736 GCGC G^CGC
KasI 7735 GGCGCC G^GCGCC
KpnI 5837 GGTACC GGTAC^C
NarI 7735 GGCGCC GG^CGCC
NheI 5629 GCTAGC G^CTAGC
NsiI 1648 ATGCAT ATGCA^T
NspV 1376 TTCGAA TT^CGAA
PaeR7I 1073 CTCGAG C^TCGAG
PflFI 3210 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3808 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 7267 ATTAAT AT^TAAT
SalI 1397 GTCGAC G^TCGAC
SapI 4065 GAAGAGC GCTCTTC(1/4)
SfoI 7735 GGCGCC GGC^GCC
SfuI 1376 TTCGAA TT^CGAA
Tth111I 3210 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3808 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1033 CCATCAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1073 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflII 2199 CTTAAG C^TTAAG
4174 CTTAAG
AocI 5638 CCTCAGG CC^T[ACGT]AGG
5965 CCTAAGG
AvaI 60 CTCGGG C^[CT]CG[GA]G
1073 CTCGAG
BalI 2138 TGGCCA TGG^CCA
6363 TGGCCA
BanII 117 GAGCCC G[GA]GC[CT]^C
940 GAGCCC
BbvCI 1102 GCTGAGG CCTCAGC(-5/-2)
6400 GCTGAGG
BclI 590 TGATCA T^GATCA
4812 TGATCA
BfrI 2199 CTTAAG C^TTAAG
4174 CTTAAG
BlpI 114 GCTGAGC GC^T[ACGT]AGC
7390 GCTGAGC
Bpu1102I 114 GCTGAGC GC^T[ACGT]AGC
7390 GCTGAGC
BsaBI 1993 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4486 GATGACTATC
BsoBI 60 CTCGGG C^[CT]CG[GA]G
1073 CTCGAG
Bsu36I 5638 CCTCAGG CC^T[ACGT]AGG
5965 CCTAAGG
CelII 114 GCTGAGC GC^T[ACGT]AGC
7390 GCTGAGC
DraI 4287 TTTAAA TTT^AAA
6549 TTTAAA
Eco81I 5638 CCTCAGG CC^T[ACGT]AGG
5965 CCTAAGG
EcoRI 1706 GAATTC G^AATTC
1777 GAATTC
FbaI 590 TGATCA T^GATCA
4812 TGATCA
HpaI 5442 GTTAAC GTT^AAC
7456 GTTAAC
MamI 1993 GATTGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4486 GATGACTATC
MluNI 2138 TGGCCA TGG^CCA
6363 TGGCCA
MscI 2138 TGGCCA TGG^CCA
6363 TGGCCA
NdeI 655 CATATG CA^TATG
4887 CATATG
SexAI 2384 ACCTGGT A^CC[AT]GGT
5301 ACCTGGT
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# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
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AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanI BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI BsmI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoNI EcoRII EcoRV EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SpeI SphI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI