Restriction map for
pF1KE2756
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BfrI BglII
BlnI BsaAI BseAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DsaI EarI
Eco81I EcoO65I EcoRI EcoT14I EcoT22I
FauI FseI FspI HpaI KasI
KpnI Ksp632I KspI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 774 CGCG CG^CG
AcsI 261 AAATTC [GA]^AATT[CT]
AcyI 1257 GACGCC G[GA]^CG[CT]C
AhdI 309 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 483 GATCC GGATC(4/5)
Aor51HI 129 AGCGCT AGC^GCT
ApoI 261 AAATTC [GA]^AATT[CT]
AspEI 309 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 394 GGCACC G^G[CT][GA]CC
BcgI 578 GCAAGGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 180 TGATCA T^GATCA
BfaI 793 CTAG C^TAG
BglI 343 GCCTTGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 175 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1257 GACGCC G[GA]^CG[CT]C
BsaI 556 GAGACC GGTCTC(1/5)
BsaWI 416 ACCGGA [AT]^CCGG[AT]
BsgI 437 GTGCAG GTGCAG(16/14)
BsiEI 350 CGGCCG CG[GA][CT]^CG
BsrBI 634 GAGCGG CCGCTC(-3/-3)
BsrDI 944 GCAATG GCAATG(2/0)
BstUI 774 CGCG CG^CG
BstXI 456 CCAGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1276 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 350 CGGCCG C^GGCCG
Eam1105I 309 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 350 CGGCCG C^GGCCG
Eco47III 129 AGCGCT AGC^GCT
Eco52I 350 CGGCCG C^GGCCG
EcoNI 624 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1308 GATATC GAT^ATC
FbaI 180 TGATCA T^GATCA
HgaI 1257 GACGC GACGC(5/10)
Hin1I 1257 GACGCC G[GA]^CG[CT]C
HincII 1285 GTCAAC GT[CT]^[GA]AC
HindII 1285 GTCAAC GT[CT]^[GA]AC
MaeI 793 CTAG C^TAG
MamI 175 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1163 TTAA T^TAA
MvnI 774 CGCG CG^CG
NdeI 1002 CATATG CA^TATG
PstI 644 CTGCAG CTGCA^G
PvuII 781 CAGCTG CAG^CTG
SfcI 644 CTGCAG C^T[GA][CT]AG
SspI 1385 AATATT AAT^ATT
Tru9I 1163 TTAA T^TAA
XcmI 189 CCAGAGTGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BcnI 380 CCGGG CC^[GC]GG
615 CCCGG
BlpI 132 GCTCAGC GC^T[ACGT]AGC
739 GCTGAGC
Bpu1102I 132 GCTCAGC GC^T[ACGT]AGC
739 GCTGAGC
BsmFI 1255 GGGAC GGGAC(10/14)
1207 GTCCC
BspHI 305 TCATGA T^CATGA
884 TCATGA
Cac8I 47 GCAAGC GC[ACGT]^[ACGT]GC
137 GCAAGC
CelII 132 GCTCAGC GC^T[ACGT]AGC
739 GCTGAGC
CfoI 130 GCGC GCG^C
971 GCGC
DraII 677 AGGACCC [GA]G^G[ACGT]CC[CT]
871 AGGACCC
DraIII 526 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1199 CACCCTGTG
EcoO109I 677 AGGACCC [GA]G^G[ACGT]CC[CT]
871 AGGACCC
HaeII 129 AGCGCT [GA]GCGC^[CT]
970 AGCGCC
HhaI 130 GCGC GCG^C
971 GCGC
HinP1I 130 GCGC G^CGC
971 GCGC
HindIII 1115 AAGCTT A^AGCTT
1138 AAGCTT
MaeII 515 ACGT A^CGT
1087 ACGT
MslI 714 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
901 CACTTCAATG
MspA1I 781 CAGCTG C[AC]G^C[GT]G
1008 CCGCTG
NciI 380 CCGGG CC^[GC]GG
615 CCCGG
PpuMI 677 AGGACCC [GA]G^G[AT]CC[CT]
871 AGGACCC
RcaI 305 TCATGA T^CATGA
884 TCATGA
TaqI 977 TCGA T^CGA
1247 TCGA
TthHB8I 977 TCGA T^CGA
1247 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbsI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EaeI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI TfiI TseI Tsp45I Tsp509I
TspRI