################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI Asp718I AspI BalI BbeI BbrPI BsaAI BsaI BseAI BsgI BsiWI Bsp1407I BspEI BspMI BsrGI BssHII BstBI BstEII BstPI CpoI DraI Eco47III EcoO65I EcoRI EcoRV HindIII KasI KpnI MfeI MluI MluNI MroI MscI MunI NarI NcoI NheI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AflII 4027 CTTAAG C^TTAAG AflIII 2906 ACATGT A^C[GA][CT]GT AhdI 3613 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3143 CCTCAGG CC^T[ACGT]AGG AspEI 3613 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1199 TGCGCA TGC^GCA BbvCI 1696 GCTGAGG CCTCAGC(-5/-2) BcgI 537 GCATCAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1070 GGATAC GTATCC(6/5) BfrI 4027 CTTAAG C^TTAAG BglI 3284 GCCTTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 4057 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 3233 TCATGA T^CATGA BspLU11I 2906 ACATGT A^CATGT BssSI 3631 CACGAG CACGAG(-5/-1) Bst1107I 236 GTATAC GTA^TAC BstXI 1430 CCAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 236 GTATAC GTA^TAC Bsu36I 3143 CCTCAGG CC^T[ACGT]AGG Eam1105I 3613 GACCAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3143 CCTCAGG CC^T[ACGT]AGG FseI 853 GGCCGGCC GGCCGG^CC FspI 1199 TGCGCA TGC^GCA HincII 769 GTTAAC GT[CT]^[GA]AC HindII 769 GTTAAC GT[CT]^[GA]AC HpaI 769 GTTAAC GTT^AAC KspI 893 CCGCGG CCGC^GG MamI 4057 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PshAI 2589 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3233 TCATGA T^CATGA SacII 893 CCGCGG CCGC^GG SbfI 3648 CCTGCAGG CCTGCA^GG SmlI 4027 CTTAAG C^T[CT][GA]AG Sse8387I 3648 CCTGCAGG CCTGCA^GG SspI 3906 AATATT AAT^ATT StuI 1005 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 236 GTATAC GT^[AC][GT]AC 2810 GTCTAC AseI 2158 ATTAAT AT^TAAT 4323 ATTAAT AsnI 2158 ATTAAT AT^TAAT 4323 ATTAAT AvrII 3096 CCTAGG C^CTAGG 4389 CCTAGG BglII 1894 AGATCT A^GATCT 4460 AGATCT BlnI 3096 CCTAGG C^CTAGG 4389 CCTAGG BsmBI 399 CGTCTC CGTCTC(1/5) 1219 GAGACG BspDI 229 ATCGAT AT^CGAT 4171 ATCGAT BsrDI 2277 CATTGC GCAATG(2/0) 4547 GCAATG BstAPI 210 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 825 GCAGAAGCTGC ClaI 229 ATCGAT AT^CGAT 4171 ATCGAT DraIII 2608 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 3199 CACCGAGTG EcoT22I 208 ATGCAT ATGCA^T 217 ATGCAT HaeII 1256 AGCGCC [GA]GCGC^[CT] 3929 AGCGCC NdeI 2366 CATATG CA^TATG 2938 CATATG NotI 895 GCGGCCGC GC^GGCCGC 3556 GCGGCCGC NsiI 208 ATGCAT ATGCA^T 217 ATGCAT PshBI 2158 ATTAAT AT^TAAT 4323 ATTAAT SacI 548 GAGCTC GAGCT^C 3476 GAGCTC XcmI 1121 CCAAAGCAACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2448 CCAGCTACGGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BamHI BanI BanII BbsI BbvI BclI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI XmnI