################################## # NO CUT ENZYMES ( 50 enzymes) # ################################## AccI AclI AflII AgeI Alw44I ApaLI AseI AsnI AvrII BbeI BciVI BfrI BglI BlnI BsaBI BspDI BsrDI Bst1107I BstBI BstZ17I ClaI EcoRV FseI HpaI KasI MamI MfeI MluI MunI NarI NdeI NheI NspV PacI PinAI PmeI PshBI Psp1406I PvuI SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccIII 2543 TCCGGA T^CCGGA AscI 3728 GGCGCGCC GG^CGCGCC AspI 987 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 3950 TGCGCA TGC^GCA BamHI 2506 GGATCC G^GATCC BbvCI 149 CCTCAGC CCTCAGC(-5/-2) BglII 3131 AGATCT A^GATCT BsaWI 2543 TCCGGA [AT]^CCGG[AT] BseAI 2543 TCCGGA T^CCGGA Bsp1407I 2303 TGTACA T^GTACA BspEI 2543 TCCGGA T^CCGGA BspHI 3323 TCATGA T^CATGA BsrGI 2303 TGTACA T^GTACA BssHII 3729 GCGCGC G^CGCGC CpoI 4771 CGGTCCG CG^G[AT]CCG DraI 3361 TTTAAA TTT^AAA EcoT22I 235 ATGCAT ATGCA^T FspI 3950 TGCGCA TGC^GCA KspI 1072 CCGCGG CCGC^GG MroI 2543 TCCGGA T^CCGGA NotI 4332 GCGGCCGC GC^GGCCGC NruI 4468 TCGCGA TCG^CGA NsiI 235 ATGCAT ATGCA^T PaeR7I 1927 CTCGAG C^TCGAG PflFI 987 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2641 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3323 TCATGA T^CATGA RsrII 4771 CGGTCCG CG^G[AT]CCG SacII 1072 CCGCGG CCGC^GG SapI 2011 GAAGAGC GCTCTTC(1/4) SspBI 2303 TGTACA T^GTACA Tth111I 987 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1927 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 188 GGTACC G^GTACC 1610 GGTACC AflIII 2996 ACATGT A^C[GA][CT]GT 3149 ACATGT AhdI 2227 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3703 GACCAGAAGTC Aor51HI 1284 AGCGCT AGC^GCT 2238 AGCGCT Asp718I 188 GGTACC G^GTACC 1610 GGTACC AspEI 2227 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3703 GACCAGAAGTC BalI 569 TGGCCA TGG^CCA 1511 TGGCCA BbrPI 1861 CACGTG CAC^GTG 2174 CACGTG BclI 3512 TGATCA T^GATCA 4406 TGATCA BlpI 3459 GCTCAGC GC^T[ACGT]AGC 4440 GCTCAGC Bpu1102I 3459 GCTCAGC GC^T[ACGT]AGC 4440 GCTCAGC BsaI 1822 GAGACC GGTCTC(1/5) 3898 GAGACC BsiWI 2456 CGTACG C^GTACG 2576 CGTACG BsmBI 2091 CGTCTC CGTCTC(1/5) 2899 CGTCTC BspLU11I 2996 ACATGT A^CATGT 3149 ACATGT BstEII 355 GGTTACC G^GT[ACGT]ACC 2647 GGTCACC BstPI 355 GGTTACC G^GT[ACGT]ACC 2647 GGTCACC CelII 3459 GCTCAGC GC^T[ACGT]AGC 4440 GCTCAGC DrdI 3895 GACGAGACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4645 GACTCCATCGTC Eam1105I 2227 GACAAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3703 GACCAGAAGTC Eco47III 1284 AGCGCT AGC^GCT 2238 AGCGCT EcoO65I 355 GGTTACC G^GT[ACGT]ACC 2647 GGTCACC EcoRI 1142 GAATTC G^AATTC 3260 GAATTC FbaI 3512 TGATCA T^GATCA 4406 TGATCA HindIII 2749 AAGCTT A^AGCTT 4792 AAGCTT KpnI 188 GGTACC GGTAC^C 1610 GGTACC MluNI 569 TGGCCA TGG^CCA 1511 TGGCCA MscI 569 TGGCCA TGG^CCA 1511 TGGCCA PmaCI 1861 CACGTG CAC^GTG 2174 CACGTG PmlI 1861 CACGTG CAC^GTG 2174 CACGTG SbfI 1098 CCTGCAGG CCTGCA^GG 3738 CCTGCAGG ScaI 3443 AGTACT AGT^ACT 4487 AGTACT Sse8387I 1098 CCTGCAGG CCTGCA^GG 3738 CCTGCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 142 enzymes) # ######################################### AatII AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI