Restriction map for
pF1KE2615
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MseI MslI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tru9I Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1008 GTCTAC GT^[AC][GT]AC
AcsI 480 AAATTT [GA]^AATT[CT]
AflIII 590 ACATGT A^C[GA][CT]GT
AlwI 876 GATCC GGATC(4/5)
AocI 550 CCTGAGG CC^T[ACGT]AGG
ApoI 480 AAATTT [GA]^AATT[CT]
AvaII 58 GGTCC G^G[AT]CC
BanII 971 GGGCTC G[GA]GC[CT]^C
BbvCI 454 CCTCAGC CCTCAGC(-5/-2)
BsaAI 255 CACGTA [CT]AC^GT[GA]
BsaI 722 GAGACC GGTCTC(1/5)
BsaWI 623 ACCGGA [AT]^CCGG[AT]
BsmAI 722 GAGAC GTCTC(1/5)
BspLU11I 590 ACATGT A^CATGT
BspMI 301 ACCTGC ACCTGC(4/8)
BssSI 772 CACGAG CACGAG(-5/-1)
BstYI 875 AGATCC [GA]^GATC[CT]
Bsu36I 550 CCTGAGG CC^T[ACGT]AGG
Cac8I 798 GCGAGC GC[ACGT]^[ACGT]GC
DpnII 876 GATC ^GATC
DraII 1047 AGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 234 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 739 GAAGAG CTCTTC(1/4)
Eco81I 550 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1047 AGGGCCT [GA]G^G[ACGT]CC[CT]
HgaI 350 GCGTC GACGC(5/10)
Ksp632I 739 GAAGAG CTCTTC(1/4)
MaeII 256 ACGT A^CGT
MboI 876 GATC ^GATC
MflI 875 AGATCC [GA]^GATC[CT]
MspA1I 313 CAGCTG C[AC]G^C[GT]G
NcoI 359 CCATGG C^CATGG
NdeII 876 GATC ^GATC
NspI 590 ACATGT [GA]CATG^[CT]
PflMI 847 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 52 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 313 CAGCTG CAG^CTG
Sau3AI 876 GATC ^GATC
SgrAI 125 CACCGGCG C[GA]^CCGG[CT]G
SmlI 931 CTCAAG C^T[CT][GA]AG
SspI 860 AATATT AAT^ATT
StuI 920 AGGCCT AGG^CCT
Tsp509I 481 AATT ^AATT
Van91I 847 CCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 875 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 258 GTAC GT^AC
569 GTAC
AlwNI 83 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
600 CAGTTCCTG
Aor51HI 422 AGCGCT AGC^GCT
890 AGCGCT
AspHI 789 GAGCAC G[AT]GC[AT]^C
800 GAGCAC
BalI 293 TGGCCA TGG^CCA
362 TGGCCA
BanI 343 GGCACC G^G[CT][GA]CC
534 GGTGCC
BbsI 49 GAAGAC GAAGAC(2/6)
655 GAAGAC
BcnI 21 CCCGG CC^[GC]GG
340 CCCGG
BpmI 660 CTCCAG CTGGAG(16/14)
978 CTCCAG
BpuAI 49 GAAGAC GAAGAC(2/6)
655 GAAGAC
BseRI 452 CTCCTC GAGGAG(10/8)
822 CTCCTC
BsiHKAI 789 GAGCAC G[AT]GC[AT]^C
800 GAGCAC
BsmI 77 GAATGC GAATGC(1/-1)
225 GCATTC
BsrFI 126 ACCGGC [GA]^CCGG[CT]
725 ACCGGC
Cfr10I 126 ACCGGC [GA]^CCGG[CT]
725 ACCGGC
DsaI 359 CCATGG C^C[GA][CT]GG
879 CCGTGG
EaeI 293 TGGCCA [CT]^GGCC[GA]
362 TGGCCA
Eco47III 422 AGCGCT AGC^GCT
890 AGCGCT
EcoT14I 359 CCATGG C^C[AT][AT]GG
923 CCTTGG
MluNI 293 TGGCCA TGG^CCA
362 TGGCCA
MscI 293 TGGCCA TGG^CCA
362 TGGCCA
NciI 21 CCCGG CC^[GC]GG
340 CCCGG
PleI 658 GACTC GAGTC(4/5)
1054 GACTC
RsaI 258 GTAC GT^AC
569 GTAC
StyI 359 CCATGG C^C[AT][AT]GG
923 CCTTGG
TaqI 519 TCGA T^CGA
896 TCGA
TfiI 325 GATTC G^A[AT]TC
744 GAATC
Tsp45I 247 GTGAC ^GT[GC]AC
378 GTCAC
TthHB8I 519 TCGA T^CGA
896 TCGA
XcmI 142 CCAGCCCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
641 CCATCATCACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI BbvI BmyI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI CfoI Cfr13I DdeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeIII MboII MnlI
MspI MvaI MvnI MwoI NlaIII
NlaIV Sau96I ScrFI SfaNI TseI
TspRI